组织培养

P1 BMSCs, P3 CCs, and P3 ADAS were implanted into collagen/hyaluronic acid scaffold at different concentrations of 10%, 20%, 50%, 80%, and 100%, and the cells were then three-dimensionally cultured in serum-free culture media. Two weeks later, SO staining and immunohistochemical staining were employed to observe the synthesis between of chondroitin sulfate and type II collagen.

取P1骨髓间充质干细胞，P3软骨细胞和P3脂肪源性成熟基质细胞种植入不同比例(10%，20%，50%，80%，100%)的胶原/透明质酸支架中，在无血清培养液中三维培养。2周后，用SO染色和免疫组织化学染色分析，观察硫酸软骨素和Ⅱ型胶原合成情况。

Objective To explore the feasibility of tissue engineered articular cartilage constructed by the technique of centrifuge tube culture and study its structure and function.

目的 探索应用离心管培养技术进行体外构建组织工程化关节软骨组织的可行性，并研究其结构和功能。

Politically, the thought on rural construction aims at the realization of political autonomy and democracy, fostering the consciousness of democracy in the hearts of Chinese peasants; economically, all experimental areas, guided by the thought, lead themselves to economic cooperation and the development of rural industry, making a significant tryout for the building of the modern economy in the rural area.The reflection on the modernization of rural social organizations and rural people leads to the understanding that the reconstruction of rural social organizations and the overall improvement of the peasants quality is the prerequisite of the modernization in the rural area. Meanwhile, this reflection helps to spread into the rural areas the conceptions of democracy and science.

民国乡村建设思想政治上以实现乡村政治的自治化和民主化为主要目标，培养了农民的民主意识；经济上引导乡村经济走合作化道路和开办乡村工业，为乡村现代经济的建设做出了有益的尝试；文化上关于乡村社会组织和人的现代化的思考，不仅让人们认识到农村社会组织重建和农民素质全方位的提高是乡村现代化的前提，而且也在一定程度上把民主和科学的理念传播到了乡村。

Using the technique of culturing shoot apex under aseptic conditions, we found that there is a sensitive stage for the sex expression of earlier flower buds, this stage is located before the second leaf full expansing.

利用黄瓜茎尖离体培养系统结合生物切片技术观察了黄瓜早期发育阶段中成花分生组织的发生及发育情况，结果表明：就黄瓜早期发育的花芽而言，可能存在着一个性别决定的敏感点，而这个敏感点位于第二片真叶展开前的某个阶段，此时的花芽绝大多数尚处在花分生组织阶段。

Objective: To provide seeding cells for vascular tissue engineering research.

第一部分组织工程血管种子细胞的培养目的：为构建组织工程血管提供种子细胞。

Part three The application of dynamic implantation and culture in vascular tissue engineering Objective: To find out a good method for vascular tissue engineering in vitro.

第三部分动态种植和培养在血管组织工程中的应用目的：寻找一种好的体外构建组织工程血管的方法。

This article is divided into three parts: Part 1 Seed cells for vascular tissue engineeringPurpose: To provide seed cells for the vascular tissue engineering research.

本课题分为如下三部分：第一部分血管组织工程种子细胞的培养和鉴定目的：为血管组织工程的实验研究提供种子细胞。

Part one Culture of seeding cells for vascular tissue engineering Objective: To provide seeding cells for vascular tissue engineering research.

第一部分组织工程血管种子细胞的培养目的：为构建组织工程血管提供种子细胞。

The thoracic duct endothelia in vitro could remain their biological properties, and could be made a tool for studing their functions and cancer metastasis mechanism by lymphatics. The positive expression of VEGF-C in cancer cells was correlated with cancer development, it seem to be that VEGF-C induce lymphoangiogenesis through its receptor Flt-4. The Flt-4 expression of lymphatics in cancer tissues was assosiated with cancer development, it is considered that there are new lymphatics in cancer tissues.

体外培养的大鼠胸导管内皮细胞可以保持体内淋巴管内皮细胞的生物学特性，可作为研究大鼠淋巴管内皮细胞功能和进一步研究癌转移机制的体外实验材料；大鼠大肠癌组织腺体的VEGF-C表达与肿瘤进展有关，推测其通过受体Flt-4诱导淋巴管生成；Flt-4在淋巴管的阳性表达随肿瘤进展增多，推测在癌组织内存在淋巴管的新生。

The last part of the report tried to supply some feasible solutions to the main problems in five aspects: enlarging the service function of the organization; improving the internal coordination of the functional strategies; strengthening the synergy of the front line teams; fostering comprehensive learning organization to raise the quality of all the staffs; being openminded to the outside world.

在综合评析的基础上，报告就解决瑞祥公司营销体系现存主要问题的可行对策进行了探讨，并提出了五个方面的对策建议：1、扩展组织架构的服务功能；2、改善职能战略的内部协调；3、增强一线团队的协同效应；4、培养全面学习型组织，提高全体员工的综合素质；5、以开放的心态看待世界。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。