组织培养

MEBT.. Method Bacteria on burn wound and alive tissue under burn wound of the 119 patients that were treated with MEBT was generally cultured and identified, when these patients were first debrided ,7 days later and before the wound healing.30 patients? randomly selected from 119 patients were enrolled in the study ,bacterial quantity and identification in burn wound tissue were carried out, bacterial epidemiology data in burn ward were analyzed in the same period.

对应用MEBT治疗的119例烧伤患者的创面于首次清创时、入院后一周、创面愈合之前作常规细菌学培养、鉴定，同时抽取其中30例进行烧伤创面坏死组织及其下活组织细菌定量计数及菌种鉴定，并与同期烧伤病房环境的细菌流行病学资料进行了相关分析。

The development of the calli and single few-celled aggregates was observed through paraffin sections.The results revealed 5 pathways of embryogenic calli origination including origination from both ends of hypocotyls,origination in inner,differentiation from non-embryonic cells,origination from outer cells of calli,and origination in the surrounding of gland.

以陆地棉品种Coker 201下胚轴为外植体诱导愈伤组织，并以介于30-50目筛之间的胚性愈伤细胞团为材料进行单个细胞团培养，采用石蜡切片法观察棉花愈伤组织诱导和分化过程及体细胞胚发生和发育过程。

MTT assay was used to evaluate the cytotoxicity of β-TCP/col on the proliferation of PDLCs. PDLCs were cultured on β-TCP/col and observed by scanning electron microscope.β-TCP/col was implated into muscles of nude mice to observe tissue reactions by histologic methods.Ⅱ° furcation involvements models in beagle dogs were established, scaffold and and scaffold with cells and cytokines were implanted into defects. Histologic observation were conducted.

通过 MTT 法测定β-TCP/col 对犬 PDLCs 增殖的影响，在β-TCP/col 上复合培养犬 PDLCs 并进行电镜观察；将β-TCP/col 植入裸鼠肌袋内并进行组织学观察；建立比格犬Ⅱ°根分叉病变模型，分别植入材料、材料/细胞复合体或材料/细胞/细胞因子复合体，进行组织学观察。

Methods Fresh NSCLC tumor samples were collected soon after the resection, and the TILs were cultured for 0,3.5,7 days respectively. PBL collected before operation.

肺腺癌病人1例，术中取新鲜肺癌组织，肺癌组织经处理后分别与自身肿瘤细胞共同培养3.5天和7天。10例健康人、T细胞株Jurkat和B细胞株Raji作为对照。

The leaf blade,leaf stalk and stem of Xanthium sibiricum were served as explants,MS medium added different kinds of hormone and proportions was used for inducing and culturing the callus of Xanthium sibiricum.

以苍耳叶片、叶柄及茎段为外植体，在MS附加不同种类和浓度激素配比的培养基上，对苍耳愈伤组织进行诱导培养，并对生长迅速、性状好的愈伤组织进行了研究。

NAA mixed with 2,4-D was more beneficial to the formation of embryogenic callus. NAA has large promoting effect on callus germination culture of Xanthoceras sorbifolia at some concentration.

结论］NAA和2，4-D混合使用有利于胚性愈伤组织的形成；一定浓度的NAA对文冠果愈伤组织萌芽培养有较大的促进作用。

Mouse fetal tissues could be cut into uniform small pieces by scissors cutting and needle grinding, which grew well in tissue culture and great amounts of cells grew from the attached small tissue clump to form primary mouse embryonic fibroblasts monolayer.

将这些组织块培养48h，大量的梭形细胞从贴壁小组织块的边缘游离出的，细胞密度大，细胞生长旺盛，很快形成原代小鼠胎儿成纤维细胞单层。

In the reforming cryopreserving method, the fetal liver tissues were permeated and equilibnzed in cryoprotectant A and B for 20 minutes, respectively, and then put into liquid nitrogen quickly after cold equilibrium at liquid nitrogen steam for 5 minutes.

改良冻存法：胎肝组织在A液、B液中分别渗透平衡20min后，在冷冻保护液B液中经液氮蒸汽冷平衡5min后迅速入液氮冻存，1周后复苏，入DMEM培养液37℃孵育1h，胶原酶消化胎肝组织，分离、收集消化后的胎肝细胞培养观察。

The sterilizing methods of the buds, leaves and petioles sprouted from the cutting shoots cultured in water in a greenhouse were investigated.

在进行愈伤组织的诱导培养时，以芽的生长点为外植体诱导愈伤组织的效果最好。

Objective To explore the feasibility of tissue engineered articular cartilage constructed by the technique of centrifuge tube culture and study its structure and function.

目的探索应用离心管培养技术进行体外构建组织工程化关节软骨组织的可行性，并研究其结构和功能。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。