组织培养

Objective: To explore the mechanisms resulting in the recurrence of urethral scar which make urethral strictures difficult to be cured, a series experiments were conducted to find potential effective factors involved in urethral scar formation and degradation, including the studies of extracellular matrix component of urethral stricture scar, the characteristics of urethral scar fibroblast, and the effects of urine on urethral fibroblast in vitro, as well as the studies to compare the difference of collagenase activity, type Ⅰ collagen and tissue inhibitor of metalloproteinase-1 (TIMP-1) in the tissues and cultured fibroblasts from normal urethra and strictured urethra respectively, and the studies to investigate the effect of antisense TIMP-1 oligodeoxyonucleotide on cell proliferation and collagenase activity of urethral scar fibroblast.

中文题名尿道瘢痕基础研究副题名胶原酶活性，TIMP-1的表达及其反义基因治疗外文题名 Experimental study on urethral scar-activity of collagenase，expression of TIMP-1，and antisense TIMP-1 gene transfection of urethral scar fibroblast 论文作者黄翔导师杨宇如教授学科专业外科学研究领域\研究方向学位级别博士学位授予单位四川大学学位授予日期2002 论文页码总数104页关键词尿道手术瘢痕胶原酶成纤维细胞尿道瘢痕馆藏号BSLW /2003 /R699 /12 目的：研究尿道瘢痕的细胞外基质的组成，尿道瘢痕成纤维细胞的生物学特性以及尿液对其生长的影响；比较胶原酶活性，金属蛋白酶组织抑制因子-1（TIMP-1）以及Ⅰ型胶原含量在尿道瘢痕和正常尿道组织及体外培养的成纤维细胞中的差异；研究反义TIMP-1寡核苷酸对尿道瘢痕成纤维细胞增殖以及胶原酶活性的影响。

The calli, derived from immature embryos, of the supersweet corn inbred line was transformed with the plasmid DNA containing Bt gene via the particle bombardment-mediated method. Through optimizing regeneration and rooting conditions in supersweet corn immature embryo culture, a stable, highly efficient plant post-transformation regenerating system has been established.

以超甜玉米幼胚为愈伤组织诱导外植体，利用基因枪法将苏云金杆菌杀虫蛋白基因转化胚性愈伤组织，通过优化再生和生根培养条件建立了稳定、高效的超甜玉米转基因再生成株体系。

It seems that the stevioside biosynthesis is related to re-differentiation of chloroplast.when cultured 60 days later,all the callus with different concentration 6-BAP do have ability to synthetize SS or RA.

培养40 d的愈伤组织仅检测到RA，表明此时的愈伤组织又恢复了合成糖苷的能力，推测与叶绿体的再分化有关。

Baaing on ecotype classification of garlic(Lu guoyi,1994),We selecte nine varietiesfrom three ecological typies and inducteded callus.

大蒜愈伤组织诱导30天后，进行继代培养，愈伤组织生长量以LS和B5培养基显著优于MS培养基。

Research results show that mechanical factors have great action on tissue-engineered culturing, it is an important domain of tissue engineering research.

研究结果表明力学因素在组织工程化培养过程中起着十分重要的作用，是组织工程研究的一个重要领域。

According to Griffing's method 1, diallel crossing was built and CA was analyzed. Variance analysis showed that there were the greatly significant difference among the both of GCA in inbreeds and SCA in crosses, respectively. The high GCA of 18-599 and 18-599 and the low SCA variance of their crosses showed the strong and stable heredity on the both characters. Taking them as parents, the generations with high and stable induction and regeneration will be gotten.

2选用在配合力、抗病性、农艺性状等玉米杂交育种性状与幼胚培养胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数等转基因工程育种性状都表现相对十分优异的18-599、18-599和综31等3个自交系，以及杂交育种性状表现优良、但转基因工程育种性状表现极差的A318，P138和R15等3个自交系为亲本，按照Griffing方法1组配双列杂交，进行Griffing的配合力分析。

The coil spring were kept constant and recorded for 0hr, 1 hr, 2 hrs, 4 hrs, 8 hrs, 12hrs, ldays, 3days or 7days. Upon completion of experiments, the maxillae were removed. The specimens were fixed in 4% paraformaldehyde in 0.1 M phosphate buffer for 24 hrs and decalcified in 10% ethylene diamine tetraacetic acid at room temperature for 5 wks.

本研究通过建立正畸大鼠牙齿移动动物模型和体外细胞培养，结合细胞力学试验方法和分子生物学测试技术，拟从组织、细胞水平以及分子生物学角度探讨ATF4在正畸牙齿移动过程中对牙周组织改建的影响及作用机制，进一步为正畸牙齿移动提供理论和实践依据。

Methods:Human embryonic tissues containing genital ridges and dorsal mesenteries were isolated and then cultured in 5 different patterns: simple mechanical disaggregation,trysinization, combination of the former 2 and with/without feeder cells.

体外分离人胚胎生殖嵴和肠系膜组织，按单纯机械分离、酶消化分离、二者结合法及是否有饲养层将组织分为 5种不同的方式培养后，用碱性磷酸酶标记，计数阳性克隆并进行比较。

Results For callus induction of leaf explants of C. orchioides, dark treatment gave better results compared to light treatment; among the media tested, the suitable phytohormone combinations were 2.0 mg/L 2, 4-D or 6-BA 1.5 mg/L 2, 4-D 2.5 mg/L, and 300 mg/L CH 0.2% AC was good for plantlet regeneration from leaf explants. The callus from leaf explants mainly originated from midrib. The parenchyma cells near epicuticle of midrib firstly were initiated to division. Then the parenchyma cells of vascular bundle sheath and mesophyll cells on each side of vascular bundle were also divided to form callus.

结果对仙茅叶片的愈伤诱导，黑暗的效果好于光照；在所试验的培养基成分中，适宜的激素配比是2.0mg/L2，4-D或6-BA1.5mg/L 2，4-D2.5mg/L，并且附加300mg/L水解酪蛋白和0.2%活性炭，对于仙茅叶片的离体成苗较好；培养后，愈伤组织主要由叶片的中脉产生，位于中脉上表皮内侧的薄壁细胞首先启动分裂，随后维管束鞘薄壁细胞及其外侧的叶肉细胞也启动分裂，参与愈伤组织的形成。

Bacteria on burn wound and alive tissue under burn wound of the 119 patients that were treated with MEBT was generally cultured and identified, when these patients were first debrided ,7 days later and before the wound healing.30 patients randomly selected from 119 patients were enrolled in the study ,bacterial quantity and identification in burn wound tissue were carried out, bacterial epidemiology data in burn ward were analyzed in the same period.

对应用MEBT治疗的119例烧伤患者的创面于首次清创时、入院后一周、创面愈合之前作常规细菌学培养、鉴定，同时抽取其中30例进行烧伤创面坏死组织及其下活组织细菌定量计数及菌种鉴定，并与同期烧伤病房环境的细菌流行病学资料进行了相关分析。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。