组织培养

Following the maturity of cultivation technology on chondrocyte and the development of medical organic materials, technology of tissue engineering come to show its superiovity in articular cartilage plerosis.

多年来，人们一直试图修复或重建软骨组织，随着软骨细胞培养技术的成熟及医学生物材料学的飞速发展，组织工程技术在软骨修复中显现出无与伦比的优势。

A systematic study was made on the induction of callus from terminal bud, cotyledon, plumular axis and the regenerated plantlets from terminal bud in Camptotheca acuminata Decne, and a large number of calli and cluster buds were obtained.

系统研究了以喜树顶芽、子叶、胚轴为外植体诱导愈伤组织的培养方法和以顶芽为外植体快速繁殖喜树的方法，获得了大量的愈伤组织和丛生芽。

The results showed that the non-embryonic callus were cultured on the MS medium contained 4.0 mg · L-1 2,4-D and 0.5 mg · L-1 6-BA, embryogenic cells appeared in it. Then the single embryogenic cells formed a proembryo composed of many cells through cell division.

结果表明，非胚性愈伤组织转入到MS + 4.0 mg · L-1 2，4-D + 0.5 mg · L-1 6-BA培养基培养后，愈伤组织中先出现一些胚性细胞；然后单个胚性细胞分裂形成一个由多细胞组成的原胚。

The germination test of Sporocarps of Microlepia hancei prantl had been done, the callus can be induced from the delicate stem of Microlepia hancei prantl; The inducement and proliferation medium of callus was MS＋6-BA2 mg/L+NAA0.2 mg/L;the optimal combination of hormones of buds differentiation was MS+6-BA 0.4 mg/L; the effect of MS medium in radication culture was better than 1/2MS, the roots of plants were all efficiently induced in MS medium without reference to adding IBA、NAA or IBA＋NAA, the rate of radication was high; the effect of seedling cultured in the mixture of perlite and turf soil was better than perlite、garden soil and river sands.the mixture of perlite and turf soil has the excellences such as ventilate、water conservation 、nourishment and so on,the survival rate was higher and seedling substance was also hale.

试验进行了华南鳞盖蕨孢子果的萌发试验，并利用幼嫩茎段进行诱导愈伤组织；愈伤组织的诱导与增殖培养基为MS＋6-BA2 mg/L+NAA0.2 mg/L；其芽分化最佳激素组合为MS+6-BA 0.4 mg/L；生根培养中MS基本培养基比1/2MS效果好，MS基本培养基不论附加生长素IBA、NAA或IBA＋NAA，均能有效地诱导植株生根，生根率高；假植以珍珠岩和泥炭土的混合物为基质效果优于珍珠岩、菜园土和河沙，具有通气、保水、营养等优点，假植成活率高，苗势健壮。

The results demonstrated that, by comparison with un-transgenic Roselle cell or callus, the critical plating cell density and critical initiating cell density of transgenic cell line were cut down 60% and 50%, respectively,the growth cycle of transgenic cell in suspension culture was shortened from 16 days to 12 days, and the specific growth rate of transgenic Roselle callus was raised 75%, the content of flavonoid compounds in transgenic Roselle cell line or callus was hardly altered.

研究结果表明，所获得的转基因玫瑰茄细胞系的临界植板细胞密度和临界起始细胞密度分别降低了60%和50%，悬浮细胞培养周期由16d缩短到12d，转化愈伤组织的比生长速率比对照提高了75%，转基因玫瑰茄细胞和愈伤组织中的花黄素含量与对照相比没有发生明显变化。

Objective: The prospective investigation of lymph secluding metastasis of T2-4 N0-phase in head-neck squamous cell carcinoma of small sample, and made prepare for constructiving plasmids.

目的：通过检测T2~4 N0头颈部鳞状细胞癌培养的肿瘤细胞及新鲜活检组织中VEGF-A、C及VEGFR-3的表达情况及构建质粒，探讨是否可以通过组织小样本前瞻性研究了解T2~4 N0患者淋巴管受累及局部淋巴转移情况并为制备VEGFR-3抗体作好准备。

The adipose derived stem cells of inbreds train of rat wereinduced to differentiate into schwann-like cells and the cells were injectedinto the acellular nerve allograff.The proliferation or adhersion of these cells on the graft were observed by inverted microscope or ScanningElectronic Microscope. Activity of cells were detected using MTT. 10mmnerve gap of inbreds train of rats in two group were bridged by tissue-engineered peripheral nerve or autogenous nerve, the effect wereappraised by naked eye, recovery rate of sciatic function index,nerve-electrophysiological, histology, Transmission ElectronMicroscopy and quantitative analysis of recovery rate of myelinated fiberpopulations,diameter of myelinated fiber and thickness of myelin sheat.

按照前述方法培养并诱导近交系大鼠脂肪干细胞向类Sehwann分化，将诱导分化的脂肪干细胞悬液注入已制备的去细胞同种异体神经支架管中，倒置显微镜观察细胞生长情况；扫描电镜下观察细胞在材料上附着情况；四唑盐比色试验测定细胞活性；取两组近交系大鼠，分别用组织工程化神经和自体神经桥接10mm神经缺损，通过大体观察、坐骨神经功能指数恢复率的测定、神经电生理的测定、组织学光镜观察、透射电镜观察、再生有髓神经纤维计数恢复率、神经纤维直径恢复率、髓鞘厚度恢复率的测定等指标评价实验效果。

But programs set, time of course, amount of curriculum, teaching condition, and teaching form are still insufficient.4The reasons why other universities and colleges do not set elective physical education course are mainly due to leaders" consciousness, quantity and quality of physical education teachers, the field and equipment.5The reform and development of elective physical education course in universities and colleges should incarnate the guideline "health first. people oriented". Teaching content should be pertinent, scientific, arranged, and systematic, so as to build up a teaching content system combined theory, skills and abilities. Set programs on base of students" choice, at the same time, give attention to the requirement of social development and students" interest, and make differential treatment according to gender. The classroom teaching should be sportful, joyful, and successful. The organizing of course could be mutual choice, compensating service, both in and out classroom. The score could be graded synthetically assess knowledge, innovation, and effect.6Strengthening the management to elective physical education course, improving teachers" comprehensive quality, making full use of fields and stadiums are all supporting conditions for the development of elective physical education course.

但是开设的项目、开课的时间、教学时数、教学条件、教学组织形式还难以满足学生学习和掌握知识和技能的需要。4、未开设公共体育选修课的高校其主要原因与领导的认识程度、体育教师的数量和质量、体育场地器材等有关。5、高校公共体育选修课的改革发展应体现&健康第一、以人为本&的指导思想：教学内容突出针对性、科学性、层次性、系统性，构建理论、运动技能、能力培养相结合的内容结构体系；运动项目设置以大学生对体育的选择性为基本前提，同时兼顾社会发展的需要和学生兴趣的需要，注重性别差异与因材施教；教学方法力求教懂、教会，体现课堂教学的娱乐性、快乐感和成功的喜悦：教学组织实施双项选择、有偿服务、课内课外相结合等多种形式；学习成绩实施知识+创新+效果的综合考核评价方法。6、加强公共体育选修课动态管理，提高教师综合素质，挖掘现有体育场馆设施的潜力，充分提高利用率是公共体育选修课改革发展的基本保障条件。

By analysing practices of Chinese enterprises strategy adjustment, the author discovers four strategi...

本文通过分析核心竞争力的确立与战略调整的目标和方向、组织结构变革与业务流程重组、企业文化的建设和品牌意识的培养以及名牌的建设等几个关键问题，提出了中国企业战略转型的对策：1、以核心竞争力为本，完成企业战略的选择与调整，实现价值的增长；2、以提高运营效率为目的，调整企业组织结构；3、以建设适合的企业文化为基础，营造良好的企业发展氛围；4、以名牌产品为载体，拓展企业的市场空间。

In view of these facts, we cocultured ESCs with striatal tissue and observed the direct inductive effect of striatal tissue.

在这些事实的启发下，我们将ESC与纹状体组织块联合培养，观察了纹状体组织块对ESC的定向诱导作用。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。