组织培养

In the favorable inoculation season, the induction percentages of meristematic globules from flowers and embryogenic calli from scalps were 15～80％ and 0～10％ respectively, depending on the cultivars and incubation conditions.

从多芽体获得胚性愈伤组织的概率大约为0～10％，胚性愈伤组织的诱导率受基因型和品种、接种时期及培养条件等因素的影响。

The central part was composed of hypertrophic chondrocytes, for which the number increased with time. The cartilaginous tissue at the time of 2 weeks' cultivation appeared strongly positive staining of collagen type Ⅱ immunohistochemistry and safranine "O", as well as strongly metachromatic to toluidine blue.

其中心为肥大软骨细胞，且随培养时间的延长而增多。2周时，软骨组织甲苯胺蓝染色呈强的红色异染反应，Ⅱ型胶原免疫组织化学染色呈强阳性，番红&O&染色呈强阳性。

Histopathologic examination showed extensive necrosis of the myomatous tissue.

病理组织学检查显示肌瘤组织的广泛坏死，微生物培养显示大肠杆菌大量生长。

When the protoplast-derived calli were transferred into the MS solid medium added with 2.0mg/L benzylamino-purine and 0.4mg/L naphthaleneacetic acid and cultivated in the codition of low illumination, after which the adventitious shoots were induced, then we could obtain numerous plantlets and could transplant them survival to pots in the greenhouse.

当将原生质体分裂形成的愈伤组织转移到附加2.0mg/L6-苄氨基嘌呤和0.4mg/L萘乙酸的MS固体培养基上，并在低光照条件下培养后，从愈伤组织上分化出了不定芽，进而发展成小植株，并移栽成活。

This experiment take one-day-old chickens as the object of study, we apply cell raise technologyand MTT method, the indirect enzyme-lined immunosorbent assay, the immunity enzymehistochemistry methods andα-naphthyl acetate esterase etc..

本实验以1日龄雏鸡为研究对象，应用细胞培养技术和MTT法、间接酶联免疫吸附实验、免疫酶组织化学和组织酸性α-醋酸萘酯酶染色法等方法。

METHODS:Nestin expression was detected in 33 cases of neuroepithelial tumors using the immunocytochemical staining. The Nestin positive and negative cells were isolated by magnetic activated cell sorting. Nestin positive cells were culutered, identified, and induced to differentiate. The expression of Nestin protein was detected in Nestin+ cells by RTPCR.

采用免疫组化法检测神经上皮肿瘤组织中Nestin的表达情况，免疫磁珠法分选肿瘤组织中的Nestin+细胞，悬浮细胞培养法进行Nestin+细胞的增殖、分化与鉴定，RTPCR法检测Nestin蛋白在其中的活性表达情况。

The reasons are probably that the micro-environment of cultivation is not perfect enough, engineered bone can not achieve the synchronization of vascularization and neurotization, and construction of the complex of tissue-engineered bone and periosteum is still lack of research.

可能的原因是细胞的培养微环境不够完善，没有实现组织工程骨的同步血管化和神经化，组织工程骨与骨膜复合体的构建目前仍缺乏研究。

The reasons are probably that the micro-environment of cultivation is not perfect enough, engineered bone can not achieve the synchronization of vascularization and neurotization, and construction of the complex of tissue-engineered bone and periosteum is still lack of research.

可能的原因是细胞的培养微环境不够完善，没有实现组织工程骨的同步血管化和神经化，组织工程骨与骨膜复合体的构建目前仍缺乏研究。仁和代写代发医学论文：提供专业的医学论文写作指导，修改，翻译，发表服务。

The optimal boron concentration for growth of pea (Pisum sativum L.) andcucumber (Cucumis Sativus L.) plants was screened by designing a series of boronconcentration in nutrient solution and the influeuce of boron deficiency or toxicityon integrity of cell membrane in different organs of the two plants was studied bymeasuring pH of the nutrient solution,〓 concentration and conductance in rootexudate solution and extracting solution of different organs from the two plants.

用不同浓度硼的营养液培养豌豆、黄瓜植株，筛选和确定在温室条件下生长所需的最适硼浓度和硼毒害浓度；进而通过对缺硼、对照和硼毒害处理后营养液pH变化，根分泌物和不同组织浸提液中K+浓度和电导率的测定，研究了缺硼和硼毒害对两种植物不同组织细胞膜完整性的影响。

Ever since, people tried their best to find a way to reconstruct articular cartilage. Following the maturity of cultivation technology on chondrocyte and the development of medical organic materials, technology of tissue engineering come to show its superiovity in articular cartilage plerosis.

多年来，人们一直试图修复或重建软骨组织，随着软骨细胞培养技术的成熟及医学生物材料学的飞速发展，组织工程技术在软骨修复中显现出无与伦比的优势。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。