组织培养

Methods: endoscopy and antral biopsies were performed in 186 cases of children with symptoms of digestive, and lesions findings.

对186例有消化道症状的儿童进行内窥镜检查，并取胃窦部活检组织作细菌培养和组织病理学检查。

Armillaria luteo-virens is a ectomycorrhizal fungus symbiotic with some species of Kobresia in Tibet Plateau, China. A strain was recently isolated from fruitbody tissue of the fungus. To determine whether the strain is the pure culture of the fungus, the internal transcribed spacer region and the first intergenic spacer region (IGS-1) of the nuclear ribosomal DNA of fruiting body and the isolated strain were sequenced and compared. Further, the 5.8S/ITS and IGS-1 sequences of the species were BLAST matched with GenBank database on homology and a phylogenetic tree was constructed based on 5.8S/ITS sequence and two phylogenetic trees were constructed separately based on two IGS-1 sequences.

首次从采自青藏高原、与高原牧草嵩草属Kobresia草本植物形成外生菌根的黄绿蜜环菌Armillaria luteo-virens子实体中分离获得一组织分离菌株，运用rDNA-ITS和rDNA-IGS-1测序技术对该组织分离菌株是否为黄绿蜜环菌的纯培养菌种进行分子鉴定，并基于黄绿蜜环菌的5.8S/ITS和IGS-1序列进行核酸序列数据库GenBank同源性检索比对、构建系统发育树。

Meanwhile metabolites of Panax ginseng and Daucus carota and their co-culture plant tissues were assayed respectively.

同时对人参愈伤组织、胡萝卜愈伤组织及其混合培养物的代谢产物进行了研究。

However, the molecular mechanisms underlying premature aging up 8-MOP/UVA are as yet unknown. In our studies, dermis of the non-lesion back skin of vulgar psoriatic patients who had been treated by PUVA and the cultured foetus dermic fibroblasts were selected to be observed.

本课题以PUVA治疗寻常型银屑病患者背部非皮损区真皮及培养的胎儿真皮成纤维细胞为对象，采用HE染色、Verhoeff染色、电镜、酶组织化学、免疫组织化学、原位杂交、流式细胞计数仪、三引物PCR、PCR-SSCP技术及PCR产物直接测序等技术和方法，研究了8-MOP/UVA对皮肤光老化形成的影响及芳维A酸乙酯的拮抗作用。

Many embryogenic calli were produced through subculture by calli induced from mature embryo of rice.

从水稻成熟胚诱导出的愈伤组织经继代培养后可以产生大量的胚性愈伤组织。

Different plant hormones and their different concentration ratios have different effects on the callus of Halenia elliptica D.

结论不同培养条件对椭圆叶花锚愈伤组织的诱导有不同的影响，其中以无菌苗的茎段为外殖体易形成愈伤组织。

Don.methodswith the stem of sterile seedling and fresh plants as exophyte, ms as a basic medium, 6-ba and naa hormone were adopted as the experimental variables.resultsms +1.0 mg / l 6-ba +1.0 mg / l naa induced the best effect of callus; higher concentrations of 6-ba with high concentrations of naa made callus densification and higher rate.conclusion different plant hormones and their different concentration ratios have different effects on the callus of halenia elliptica d.

方法以椭圆叶花锚种子培养的无菌苗的茎和新鲜植株的茎段为外殖体，ms为基本培养基，分别以6-ba和naa两种植物激素为变量进行实验研究。结果ms培养基+1.0 mg/l 6-ba+1.0 mg/l naa对诱导椭圆叶花锚愈伤组织的形成效果最佳；高浓度的6-ba配合较高浓度的naa诱导的愈伤组织致密，出愈率高。

Methods The tissues from human ovarian carcinoma biopsies were cultured in RPMI1640 media. After growing to 90% confluence, the cells were indigested for subculture, whose characteristics, including morphology, growth kinetic and tumorigenecity in nude mice, were studied.

取手术切除的卵巢癌组织，进行组织块培养法，待长满90%汇合面后消化传代培养，并进行形态学、生长动力学及致瘤性等生物学特性分析。

All selected tissues from different positions of the same Lactarius deliciosus basidiocarp can germinate mycelium on PDA. It showed that different position tissues could be used in isolation culture.

在PDA培养基上，Lactarius deliciosus子实体不同部位的组织均能萌发长出正常菌丝，说明不同部位的组织都可用于分离培养。

Effect of percutaneous penetration ,including influence of different formulations on penetration,and lipolytic activity of Siloxanetriol alginate were studied using the techniques of percutaneous penetration in vutro and adipose tissue incubation in vitro.

利用体外经皮渗透和脂肪组织体外培养技术，探讨了硅烷醇C 经皮渗透和配方的促渗透，以及对脂肪组织的脂解作用。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。