组织培养

The cells infected and noninfected were combined with PLGA at a concentration of 5×106cells/ml to construct tissueengineered bones respectively.They were further implanted into 8 rabbits subcutaneously using PLGA as blank control(8 implants per group). Six weeks after surgery, the implants were evaluated with histological staining and computerized analysis of new bone formation.

方法］PLGA与腺病毒Ad-BMP-7感染后的兔MSCs共培养，电镜观察了解细胞在PLGA上黏附、生长和合成分泌骨基质成分的情况；将转染基因的细胞和未转染基因的细胞分别与PLGA支架复合，构建组织工程化骨并植入体内，通过组织观察新骨形成情况。

AIM: To observe the expression and distribution of C5a receptor(C5aR) on epiretinal membranestissue from patients with proliferative retreous body retina lesion and cultured retinal pigmentary epithelial cells.

目的 ：检测手术中取出的增生性玻璃体视网膜病变患者的视网膜前膜组织及培养的人视网膜色素上皮细胞中的C5a受体(C5aR)的表达及分布，阐明C5a受体在发生PVR的病理过程中可能的作用。方法：手术取出ERM组织，常规制备石腊切片。

The clinical presentation and investigations including histopathological examination of a biopsy and negative bacterial cultures were consistent with the diagnosis of pustular psoriasis von Zumbusch type.

临床表现和研究（包括活检组织的组织病理学检查和阴性细菌培养）符合脓疱型银屑病诊断标准。

The "regulable osteoinduction microcirculation/microenvironment system in vivo" has characteristics as follows: the culture conditions are controlled. For example, the release of a variety of growth factors can be controlled stably and quantitatively at bone defect site. Early nutrition and revascularization in the center of tissue-engineered bone can be effectively solved; some shortcomings resulted from construction of tissue-engineered bone in vivo, including secondary injury, high level technical requirements, and complex operation, can be avoided; vascularization and osteogenesis can be constructed simultaneously; simple safe and efficient operation.

可调控的体内灌注诱导成骨微循环系统具有以下特点：可以控制诱导培养条件，如调控各种生长因子微量稳定的缓控释放；能有效解决大块组织工程骨中心部位的早期营养和血运重建难题；避免了组织工程骨体内构建存在的二次损伤，需要较高的技术条件、操作较为复杂等缺陷；实现血管化与成骨的同步构建；操作简便，安全高效。

The central part was composed of hypertrophic chondrocytes, for which the number increased with time. The cartilaginous tissue at the time of 2 weeks' cultivation appeared strongly positive staining of collagen type Ⅱ immunohistochemistry and safranine "O", as well as strongly metachromatic to toluidine blue.

其中心为肥大软骨细胞，且随培养时间的延长而增多。2周时，软骨组织甲苯胺蓝染色呈强的红色异染反应，Ⅱ型胶原免疫组织化学染色呈强阳性，番红"O"染色呈强阳性。

A new parallel-epiphyseal cartilaginous tissue was found in the subcutaneous tissue 4 weeks after operation. The tissue was stained with HE, toluidine blue, safranine O and collagenⅡ. A large number of chondrocytes and a cartilage lacuna-like structure were observed under a microscope with no obvious inflammatory reaction around the calcium alginate and polylysine microspheres.

结果 体外培养时微球中骺板细胞生长、增殖情况良好；移植皮下后第4周取材可见外形呈类骺软骨样组织块，行HE、甲苯胺蓝、番红"O"及Ⅱ型胶原等染色，镜下观察可见大量软骨细胞及类软骨陷窝样结构，植入的海藻酸钙微球周围组织无明显炎症反应。

In fact, from Wahaha management systems and practice, we can see that the management strategy: First, to implement a win-win aggregators institutional, Wahaha in China's 31 provinces to strictly selected more than 1,000 dealers, formed covering nearly Every town in China a joint marketing system, and use margin approach to building confidence between the parties, this approach dramatically changed the Wahaha trade forms of organization, Wahaha's chairman and general manager Zong said that this organizational form of "aggregators body"; Second, the implementation of differential pricing system, Wahaha's existing sales network of the company itself, a special dealer, special two dealers, secondary dealers, three dealers, retail terminals, and strict price control system; 3, comprehensive incentives, Wahaha's rebate is not a single incentive sales rebate of such direct incentive, but rather to include a variety of promotions and other indirect incentives, including a comprehensive incentive measures; 5, strict accountability and developed a regional strict reward and punishment system, Wahaha and dealer signed a contract to the authorized dealers to strictly limit the sale of the area to prevent the dealer cross-regional sales in order to strictly protect the dealers around the interests; 6, and distributors to establish deep feelings, Wahaha and distributors Once the partnership working to develop a long-term strategic partner; 7, emphasis on marketing, team building, Wahaha throughout the country only more than 3000 sales staff Why so few sales staff can help the company to complete more than 32.5 billion yuan in annual sales, which Wahaha focus on team-building and marketing are inseparable.

其实，从娃哈哈的管理制度和实际操作中我们可以发现其管理策略：一、实行双赢的联销体制度，娃哈哈在全国31个省市严格选择了1000多家经销商，组成了几乎覆盖中国每一个乡镇的联合销售体系，并采用保证金的方式建立双方的信任，这种方式极大地改变了娃哈哈的交易组织形式，娃哈哈公司董事长兼总经理宗庆后称这种组织形式为"联销体"；二、实行级差价格体系，娃哈哈现在的销售网络构成是公司自身、特约一级经销商、特约二级经销商、二级经销商、三级经销商、零售终端，并实行严格的价格管理体系；三、全面的激励措施，娃哈哈的返利激励并不是单一的销量返利这样的直接激励，而是采取包括各种优惠活动等间接激励在内的全面激励措施；五、实行严格的区域责任制并制定了严明的奖罚制度，娃哈哈和经销商签订的合同中给特约经销商限定了严格的销售区域，防止经销商跨区域销售，以严格保护各地经销商的利益；六、与经销商建立深厚的感情，娃哈哈和经销商一旦确定合作关系就致力于发展成为长期的战略伙伴；七、重视营销队伍的建设，娃哈哈在全国各地只有3000多销售人员，为什么如此少的销售人员可以帮助公司完成超过325亿元的年销售额，这与娃哈哈注重营销队伍的建设和培养是分不开的。

The effects of sorbitol, ABA and 48 h dehydration on the growth of long-term subcultured callus in Maize were investigated.

以长期继代培养的玉米愈伤组织为材料，研究了山梨醇、ABA和干燥48h对愈伤组织生长的影响。

Based on extrapolated organ shape criteria,we select ears that are in uninucleate period,carry out pretreatment in a 6~8℃ low-temperature condition for about 12 days, and callus induction rate can be enhanced in this way.② 1~2mm diameter should be the appropriate size for translocated callus,and plants grow to 4cm breeding for mature plants should be conducted in a timely manner.③ Get well down with sterilization,reduce contamination and strengthen greenhouse management,etc.

根据器官形态指标外推法选择花粉发育处于单核靠边期的幼穗，进行6~8℃低温预处理12d左右，可提高愈伤组织诱导率；②愈伤组织转移大小以直径1~2mm为宜，当绿苗长至4cm左右时及时进行壮苗培养；③做好接种材料、室内、人员的消毒和卫生工作，降低污染率，加强温室等的管理。

RESULTS: Tissue engineering should solve the design and preparation of molecular scaffold, tissue vascularization and dynamic culture of cell on the scaffolds in vitro.

结果 组织工程应解决分子支架的设计与制备、组织血管化和细胞在支架上进行体外动态培养问题。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。