组织培养

ABSTRACT Antitumor Activity of Dendritic Cells Activated with gP96 Peptide Complex Department of Thoracic Surgery, Peking University People's Hospital Objectives: To investigate the expression level of gP96 in human lung cancer samples; purify gP96-polypeptide from human and mouse lung adenocarcinoma cell lines; prepare tumor vaccine by in vitro gP96-polypeptide stimulation of monocyte-derived dendritic cells and test its efficiency both in vitro and in mice models.

研究人肺癌组织热休克蛋白gP96—多肽复合物的表达水平；肺癌细胞株来源的肿瘤组织gP96—多肽复合物的提取和鉴定；外周血来源树突状细胞培养和鉴定：将gP96—多肽复合物和DC体外共同孵育制备疫苗，在体外激活T淋巴细胞的能力及在小鼠体内的抑制肿瘤生长作用的研究。

a simple and stable method for isolation and cultivation human adipose-derived adult stromal cells is successfully established , which provides the foundation for future usage of human adas cells in tissue engneering.

我们建立了一种自人体脂肪组织分离、培养人脂肪基质细胞简便稳定的方法，为其应用于组织工程提供了基础。

MTT method was used to detect the proliferation of the cells. Cell alkaline phosphatase activity and expression of collagen type I were assessed.

另以组织化学和免疫组织化学方法检测传代培养的SD大鼠骨髓基质成骨细胞在PMMA驻极体表面的增殖和分化情况。

In plant organs, tissues, cells cultured in vitro, conditions can also be suitable for callus growth.

在植物器官、组织、细胞离体培养时，条件适宜也可以长出愈伤组织。

Osteoblasts were collected from the rabbit fetus skull by collagenase digestion associated with tissue nubbles cultivation and cultured in vitro.

酶消化-组织块联合培养法体外培养胎兔颅骨成骨细胞：取新西兰大白兔胎兔颅骨骨块，制成2mm 2mm骨块，Ⅰ型胶原酶室温下消化。

The method of centrifuge tube culture can be used as a constructing technique of tissue engineered cartilage without scaffold material.

结论在无支架材料条件下，采用离心管培养技术培养的人胚胎关节表面软骨细胞可以构建组织工程化关节软骨，而且接近正常关节软骨的形态和功能。

The method of centrifuge tube culture can be used as a constructing technique of tissue engineered cartilage without scaffold material.Cartilage,articular; Histology; Biomedical engineering; Cell culture

结论在无支架材料条件下，采用离心管培养技术培养的人胚胎关节表面软骨细胞可以构建组织工程化关节软骨，而且接近正常关节软骨的形态和功能。

As a troop train the cadets, the military barracks in 2008 to strict discipline, strict management is known, a qualified military personnel should first submit to the party and the organization's leadership, discipline, to develop good habits to develop style.

作为一名部队培养的军校学员，八年的军营素以严明的纪律、严格的管理而著称，一名合格的军人应首先服从党和组织的领导，遵守纪律，培养良好的作风养成习惯。

The methods of sticking tissues piece to culture primary hFCECs and mouse 3T3 fibroblast conditioned medium to culture passage hFCECs were established;2.The Proliferative capacity of corneal limbus hFCECs is better than central hFCECs.3.3T3 conditioned medium could markedly promote the proliferation of epithelial cells

结论一、组织块贴壁法原代培养人胎儿角膜上皮细胞以及小鼠3T3成纤维细胞条件培养液传代培养方法基本建立；二、人胎儿角膜缘部上皮细胞增殖能力优于中央部；三、3T3细胞条件培养液能促进角膜上皮细胞的增殖。

LDH in culture fluid, SOD, XOD, free calcium in L-6TG cell and mitochchondral respiration were evaluated in each group; apoptosis was detected by flow cytometer with PI staining method and agarose gel electrophoresis; the immuneohistochemical method was used to determine the expression of bcl-2 and Caspase-3 ; the morphoiogic changes were observed with microscope.

将培养的L-6TG大鼠肌母细胞随机分为3组：①正常对照组，②缺血再灌注组（1R组），③缺血预处理组，观测了培养上清中LDH、细胞内SOD、XOD、Ca(上标 2+)含量的变化；采用MTT法检测线粒体的功能；利用流式细胞仪和细胞DNA电泳结果检测细胞凋亡情况；采用免疫组织化学的方法检测bcl-2及Caspase-3的蛋白表达情况，结合自动图像分析系统对其结果进行定量分析；在光镜下观察细胞的形态学改变。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。