组织培养

The ligamentous scaffold material were made from the PVA fibers by braiding technique. The mechanical properties of the scaffold materials were measured using the electronic materials testing machine. Human ACL cells were isolated and cultured by tissue explant and collagenase digestion methods, the morphous characteristics of cells and the secretion of collagen protein were measured. In order to observe the biocompatibility between the material and cells, ACL cells were in vitro expanded and seeded on the scaffold materials.

方法］用聚乙烯醇纺丝纤维编织构建韧带支架材料，在电子拉力机上测试该支架材料的力学性质；用组织块和胶原酶消化培养法在体外分离培养人前交叉韧带细胞并对细胞的生长形态和分泌胶原蛋白的特征进行检测，细胞经体外扩增后种植于编织构建的聚乙烯醇韧带支架材料上观察。

Successful induction of the callus tissues and establishment of suspension culture of Marchantia polymorpha L. provide a new pathway for exploring the pharmaceutical moss to produce secondary metabolism and a new theory guidance for suspension culture of moss cell in a large scale.

结论地钱愈伤组织的诱导成功和悬浮细胞培养条件的建立，为探索药用苔藓植物次生代谢产物生产提供新的途径以及对苔藓植物细胞规模化培养提供新的理论指导。

Objective: To explore the natural differentiation of neural stem cells and the induced differentiation of neural stem cells by co-culture with mesencephalic cells.

目的：用体外培养方法探索小鼠胚胎神经干细胞的自然分化及与中脑组织细胞共培养下的诱导分化。

Our compang paid attention to training talents, insisted the principle of "Appointing people withabilities", trained and elevated large number of middle and high-lever managers and high-grade design talents, organized professionals to study abroad for several times, encouraged designers to innovate nervily, and hold the quality of producing technology and products strictly.

公司注重人才培养，坚持&任人唯贤&的原则，培养和提拔大批中高层管理人才和高级设计人才，多次组织专业人员出国考察学习，鼓励设计人员大胆创新，同时严把制造工艺和产品质量关。

Adding fresh medium to cultures, when they had been cultured for 5-8 days, could promote cell sustained division, after which, adding a medium with lower concentration osmatic substance or without osmatic one could futher stimulate the cell division and ensure the active growth of the cell masses.

采用液体浅层培养方法，对埃斯基和雷蒙特红豆草的原生质体进行了培养。埃斯基花茎愈伤组织的原生质体在VK和Kao＇s培养基中2-6天分裂，2周内形成细胞团，19天时的分裂率和克隆率分别为71.41-76.19％、64.78-67.03％。

We demonstrated that the human mesenchymal cells outgrowing from cultured human pancreatic endocrine or exocrine tissue are pancreatic mesenchymal stem cells that propagate from contaminating pMSC.

我们证实体外培养的胰腺内分泌部或外分泌部组织可获得的人类间充质细胞是一类胰腺间充质干细胞，其来自培养体系中pMSC的扩增。

The immunocytochemistry was used to identify the RASMC.RESULTS: The RASMC were successfully cultured by the adherent method of tissue explants and grown in the "peak- valley" mode. The cultured RASMC were fusiform shape with a big oviform or round nuclei rich in cytoplasm. Immunohistochemical method ( S-P method) showed strong expression of a- smooth muscle actin.

结果：组织块贴壁法成功培养出SD大鼠胸主动脉平滑肌细胞，培养的细胞呈典型&峰-谷&样生长，HE染色细胞呈梭形，胞浆丰富，核大而圆或椭圆，免疫组化S-P法检测a-平滑肌肌动蛋白单克隆抗体呈强阳性表达。

Methods Pancreatic cancer cell panc-1 was treated with oxamate for 48 h,the mitochondrial membrane potential was measured by flow cytometry(Rodamin-123),and the activity of intracellular lactate dehydrogenase was studied by enzymohistochemistry.

方法体外培养人胰腺癌细胞panc-1，分别加入不同浓度的草氨酸盐培养48 h后，行酶组织化学染色及罗丹明123染色，观察细胞糖酵解关键酶乳酸脱氢酶活性及线粒体膜电位。

METHODS: the supernatants of cultured spleen's lymphocytes induced by ConA were injected into mice peritoneally,kinetics of plasma corticotropin releasing hormone,adrenocorticotropic hormone and corticosterone in mice were tested at different time (0.5,1,2,4,6 h),or tissue of hypothalamus pituitarium or adrenal was stimulated with supernatants induced by ConA,and the secretion of CRH, ACTH and CORT were observed.

采用ConA诱导淋巴细胞培养上清液给小鼠腹腔注射，测定不同时间(0.5，1，2，4，6 h)的小鼠血浆中CRH，ACTH，皮质酮产生的动力学，同时在体外用ConA诱导上清液分别与下丘脑，垂体，肾上腺组织共同培养后观察CRH，ACTH，CORT分泌情况。

Tendon cells 5 x 10(4/ml derived from phalangeal flexor tendon of SD rats were co-culture with CPPF scaffold or CPPF scaffold resurfaced with collagen type-I within 1 week. The co-cultured specimens were examined under optical and electric scanning microscope.

将体外培养 SD大鼠原代趾屈肌腱细胞，以 5×10 6/ ml浓度与 CPPF辫状支架或型胶原涂层的 CPPF辫状支架进行体外混合培养，组织学观察材料与细胞的吸附情况。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。