组织培养

MSC can be obtained by percoll fluid and density gradient centrifugation, this cells kept proliferation and differentiation ability in vitro; MSC can differentiate into chondroblast by lightly centrifugation and chemical definition medium, after one generation, the differential cells kept the phentype of chondroblast, they are a kind of ideal seed-cells for cartilage tissue engineering.

经percoll液密度梯度离心法能获得纯度较高的人骨髓间充质干细胞，这种细胞在体外培养体系中保持旺盛的增殖能力；经轻度离心和化学限定培养基法可诱导骨髓间充质干细胞向成软骨方向分化，增殖旺盛，可传一代培养，是软骨组织工程理想的种子细胞。

This investigation include two parts: lThe primary culture, chondroblast induction and evaluation of MSC. 2The chondroblast cultured with compound porous materials PDLLA and PDLLA/chitosan.

第一部分人骨髓基质干细胞的原代分离、体外培养、成软骨诱导及鉴定目的：了解骨髓基质干细胞原代培养的特性及增殖能力，探讨轻度离心和化学限定培养基法诱导骨髓基质干细胞向成软骨方向分化的作用；为软骨组织工程提供足量、合适的种子细胞。

In contrast to conventional methods, a novel cell sheet-based technique has been proposed .This technique intend to achieve high cell density and function by layering confluent viable cell sheets without the use of acellular scaffolds that limit mass transport. Using thermo-sensitive cell culture surfaces, confluently cultured cells spontaneously detach as intact sheets simply by reducing the culture temperature.

与传统方法相比，有学者提出了一种新的以细胞层为基础的组织工程技术，这种技术作为一种新的选择，利用温敏性培养皿，经简单的温度变化，可以非损伤性的一起获得完整的培养细胞层及其所沉积的细胞外基质，从而避免了使用蛋白水解酶。

In order to investigate the role of ICAM-1 and VCAM-1 in the pathogenesis of glomerulonephritis, ICAM-1 and VCAM-1 expression were evaluated from both protein and gene level by in vivo and in vitro study, We have conducted (1) immunocytochemical analysis and in situ hybridization to examine the alteration in expression of ICAM-1 and it's relationship with interstitial infiltrating cells and TNFα; A total of 64 renal biopsies were classified in three groups according to the degree of cellular proliferation and infiltration in glomerulus;(2) indirect immunofluoresence and immunoblotting. methods to detect the VCAM-1 level both in renal tissue and in serum from the patients of lupus nephritis (17cases) and crescentic nephritis (4 cases);(3) Cell ELISA and northern blot technique to study the effects of TNFα and IL-1β on ICAM-1 and VCAM-1 surface expression and gene expression by cultured human mesangial cells .

为了探讨ICAM-1和VCAM-1在肾小球疾病中的作用，本文从蛋白质和基因两个水平，整体（研究ICAM-1时根据肾小球内细胞增生和浸润程度，将64例病人分为A.B.C三组）和细胞培养两个方面，做了如下工作，（1）利用免疫细胞化学和原位杂交技术观察了ICAM-1在64例肾小球疾病患者中的表达及其与间质浸润细胞、TNFα之间的关系；（2）利用间接免疫荧光和膜免疫印迹方法检测了VCAM-1在17例狼疮肾炎和4例新月体肾炎病人肾组织及血清中的表达；（3）利用细胞ELISA和Northern杂交技术研究了IL-1β或TNFα对体外培养的人肾小球系膜细胞ICAM-1和／或VCAM-1表面表达及mRNA表达的调节作用。

The biotechnology production efficiency of crocin II, which has anti-cancer activity, was improved in a solid-liquid two-step cell culture in which the callus cells obtained from the solid medium were transferred to the liquid medium.

为提高现代生物技术生产藏红花素的产率和效率，该研究采取固液两步培养法将在固体培养基中获得的藏红花愈伤组织转移到液体培养基中进行生物合成，结果显示生物反应器比摇瓶培养更有利于生物量的积累，也更有利于2号藏红花素的合成。

Panax ginseng and Daucus carota plant tissues were co-cultured by suspension way, and the best medium for suspension co-culture was screened in this study.

本实验对人参和胡萝卜两种植物组织进行悬浮混合培养，筛选出悬浮混合培养的最佳培养基。

The cooperation of 8-MOP/UVA could induced dermis of the non- lesion back skin of vulgar psoriatic patients and the cultured dermic fibroblasts showed up photoaging-characteristic changes of histology and biology rapidly, it is feasible to establish human skin photoaging models by selected the human skin of sun-protected sites treatment with PUVA and cultured dermic fibroblasts treatment with 8-MOP/UVA as studied objects.

1.8-MOP/UVA可诱导寻常型银屑病患者背部非皮损区真皮及培养人类真皮成纤维细胞迅速出现具有光老化特征性的组织学及生物学特性改变，以PUVA治疗的非暴露部位皮肤及8-MOP/UVA作用的培养真皮成纤维细胞作为研究对象建立人类皮肤光老化模型是可行的。

Methods The NGF, BDNF, and NT3 genes of rats were cloned, the eukaryote expression vectors were established, the three kind of recombinant vectors were used to transfect astrocytes, the positive cloned cells were cultured dilatedly after G418 sifting; using supernates of culture liquid of astrocytes modified by gene to culture PC12 or TrkB-PC12, the expression and its level of gene target cells aimed genes were measured by Western blotting or immunohistochemical method.

克隆大鼠NGF、BDNF和NT3基因，构建真核表达载体；3种重组载体分别转染星形胶质细胞，G418筛选后获得阳性克隆细胞进行扩大培养；取基因修饰星形胶质细胞培养液上清培养PC12细胞或TrkB-PC12细胞；用Western blotting杂交或免疫组织化学方法检测基因靶细胞目的基因的表达及其水平。

In this paper, the histogical studies of Elaeagnus mollis stem segments cultured on MS with higher BA and lower NAA from 0 to 30 days were researched.

本文对翅果油树大宫灯型茎段培养在MS附加6－BA较高、NAA较低浓度的培养基上培养0～30d的组织学变化进行了研究。

Aim:To explore the feasibility of neural stem cells derived from both fetal rat ependyma and adult rat bone marrow.

目的 ：探寻胚鼠室管膜和成鼠骨髓分离培养神经干细胞的可行性。方法：将分离的胚脑室管膜组织或成年骨髓细胞分别特殊培养。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。