组织培养

Above research indicates that it is a fundamental countermeasure to implement the strategy of prospering ages Yanbian autonomous through scientific education, that the management of human resources in Yanb ian must adopt. We must adopt following measures: first, promote the science and technology structural adjust; foster qualified scientists and technicians of agriculture and engineering technicians; devote major efforts to developing education run by the local people; widen the resources of educational fund; develop higher education, especially postgraduate education; make vocational education universal; strengthen on-the-job training of the talent; attach great importance to the cultivation of the talent of countryside and ethnic cadre. Second, recommend urgently needed higher talent managers and technicians on the basis of remaining and making use of personnel; establish creative mechanisms; conform to creative resources; cultivate creative main members; make creative environment. Third, strengthen programme; forecast organization structure in the future; work out a plan of balance between supply and demand; work out a plan of advertising replenish of human resources; work out a plan of training and employ ing staff. Fourth, strengthen the establishment of soft environment in the whole society. Last, build up the patten of management of human resources based on people's ability.

研究表明，实施科教兴洲战略是知识经济时代延边人力资源管理应采取的根本措施，在此基础上，延边人力资源管理中应采取的措施有：促进科技结构调整，重点培养农、林科技人才和工程技术人才，大力发展民办教育，拓宽教育资金来源，发展高等教育的同时，重点发展研究生教育，普及职业教育，加强人才的在职教育培训，重视农村人才、民族干部的教育培养；盘活现存，既要留人，又要用人，在此基础上引进急需的高层次的管理人才和技术人才；建立创新机制，整合创新资源，培育创新主体，营造创新环境；加强规划，预测未来的组织结构，制定人力供求平衡计划，制定人力资源征聘补充计划，制定人员培训计划，人力使用计划；增强整个社会的软环境建设；建立以人为本，以能力为平台的人力资源管理模式。

MAIN OUTCOME MEASURES: The seeded cells were observed under inverted microscope; at 1, 2, 3 weeks after seeding, the BMSCs were treated with 4% paraformaldehyde and stained with hematoxylin-eosin; The protein content in seeded cells was determined by bicinchoninic acid assay, and the content of DNA was quantified using Hoechst33258 assay at 5, 10, 14 days after culture.

主要观察指标：倒置显微镜下观察细胞形态；于培养1，2，3周采用40 g/L多聚甲醛固定，常规组织切片，苏木精-伊红染色；在培养5，10，14 d用Hoechs33258荧光法定量测定细胞内DNA含量，BCA法测定蛋白质含量。

Under the solid culture conditions,the effects of source of explants,medium,phytohormone,inoculating quantity and culture days on growth of calli and content of flavonoid in Cyclocarya paliurus were studied.

在固体培养条件下，研究了不同外植体来源、培养基、植物激素、接种量、培养时间对青钱柳愈伤组织生长和黄酮含量的影响。

Using the crude toxin, the research investigated the effect of Phytophthora capsici on the leaf of pepper, the germination of pepper seeds, the growth of seedlings, the growth of pepper radicel, and the callus of pepper.

用胡萝卜汁培养液培养辣椒疫病病原菌，其培养滤液为辣椒疫病病原菌粗毒素，测定了粗毒素对辣椒叶片、辣椒种子发芽率、辣椒种子胚根生长、辣椒幼苗生长和辣椒愈伤组织的影响。

METHODS: Chitosan scaffolds were prepared by 6% chitosan based hydrolgels produced from acetic acid and chitosan. Schwan cell of neonatal rat sciatic nerves was cultured using enzymatic digestion adherent method.

以6%壳聚糖乙酸水溶胶制备壳聚糖支架，取乳鼠坐骨神经采用酶消化后组织块贴壁培养的方法培养许旺细胞。

By using the technique of tissue culture, we successfully obtained the cell from goldfish fin, skin, eye, heart, spleen, spermary, swim bladder and kidney. Among them, GFFhas the strongest proliferating ability.

采用组织块法和胰蛋白酶消化法对金鱼细胞进行体外培养和传代，研究了高倍稀释、培养液中的小牛血清浓度以及冷冻保存等因子对显示出较高的增殖能力的来源于鳍的细胞GFF的影响进行了试验；并对其进行金鱼疱疹病毒接种、病毒传代培养等试验和电子显微镜观察。

ADSCs are a promising source for tissue engineering for their characteristics such as easy acquirability, extensive proliferation, multilineage differentiation, etc.

研究结论 1、ADSCs具有易于获取，能够在体外大规模培养和扩增，性质稳定，多潜能分化，培养扩增不需要大量的血清等优点，是组织工程研究中良好的种子细胞来源。

Objective To study the feasibility of engineering auricled neocartilage with chitosan/polylacticacid-polycrylactone network scaffolds and to search the difference between dynamic and silent chondrocytal culture techniques.

目的 探讨采用表面修饰过的壳聚糖聚乳酸(polylacticacid ，PLA)聚乙内酯(polycrylactone ，PCL)及牛跟腱胶原海绵作为支架培养组织工程化耳廓软骨的可行性，比较动态与静态两种不同培养方法对软骨生长的影响。

By Percoll fluid and density gradient centrifugation,the MSC was obtained;after the cells filled the bottom of vessel,subcultured them,when they subculture in third generation ,redigested them,500 R/min centrifugate,alter the

经percoll液密度梯度离心法能获得纯度较高的人骨髓间充质干细胞，这种细胞在体外培养体系中保持旺盛的增殖能力；经轻度离心和化学限定培养基法可诱导骨髓间充质干细胞向成软骨方向分化，增殖旺盛，可传一代培养，是软骨组织工程理想的种子细胞。

Articular chondrocytes from the knee joints of the A group were separated respectively to fragments, and then digested by enzymes, both the chondrocytes and the uncomplite digested tiny chondral tissues have been cultured in vitro for about 2 weeks.

分别取A组9只兔子的双侧膝关节股骨滑车及部分髁部的软骨，在体外进行分离和消化，未完全消化的软骨组织加软骨细胞在培养瓶内一同培养2周后配制成浓度为1.6×10~8/ml的软骨细胞悬液，等分装于两个EP管中。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。