组织培养

METHODS Human secondary follicles were isolated from ovarian tissues obtained from cancer patients and grown in vitro within a bio-engineered culture system for 30 days.

方法从癌症患者的人卵巢组织中分离出次级卵泡，并在体外的生物工程培养体系中培养30天。

Methods HPDLFs were primary cultured from tissue explants, and the cells of the 5th to 8th passages were used after immunohistochemical identification of keratin and vimentin expressions. The cells were divided into 5 groups and treated with TP at 1, 0.5, 0.25, 0.125, and 0.0625 mg/ml, respectively, with another group without TP treatment as the blank control group. Cell counting and MTT colorimetric assay were performed to assess the cell proliferation, and flow cytometry was employed to determine the DNA content of the HPDLFs.

采用组织块培养法培养原代人牙周膜成纤维细胞并传代，经免疫组化SABC法检测角蛋白和波形丝蛋白鉴定，取5~8代细胞用于实验；按茶多酚不同浓度分1mg/ml、0.5mg/ml、0.25mg/ml、0.125mg/ml、0.0625mg/ml组和空白对照组，采用细胞计数法、MTT法检测细胞增殖情况，流式细胞术检测细胞DNA含量。

Objective: To study osteogenic capability of rabbit bone marrow stromal cells and biological characteristical identification in vitro in order to explore the appropriate methods of osteoblast culture in vitro and to select an idea source of seed cells for bone tissue engineer .

目的：研究兔骨髓基质细胞在体外培养条件下的成骨能力及生物学特性，探讨简便易行的成骨细胞体外培养方法，为组织工程选择理想的种子细胞来源。

METHODS: Human periodontal ligament cells were exposed for 1 hour to 0.25% chloromycetin, normal saline and DMEM culture medium.

采用组织块法体外培养人牙周膜细胞，取第5代培养细胞。

The suspension cells of the heart at E11 were respectively cultured in co-culture system based in the stromal cells from the heart, the fetal liver and AGM region with the same conceptus age, and c-kit+ cells were analyzed by flow cytometer, estimating the effect of different stromal cells on HSCs\' amplification.

将培养24h后的E11心脏悬浮细胞分别置入由同期的心脏内皮细胞、胎肝基质细胞和AGM区基质细胞构成的共培养体系中，于0h， 24h和48h分别检测c-kit+细胞比率，研究各组织基质细胞对HSCs扩增的影响。

On one hand, the open and radiative culture benefits to gathering and exchanging information which helps to nurture the insight, while the centralized and consubstantial culture benefits to the harmony and consolidation of organizations which helps to nurture the implement competence, on the other hand, corporate culture especially core values have an effect on the selection and formation of potential core competence.

一方面，开放型发散式的文化有助于信息的吸收和流通，从而有利于培养企业对未来的洞察力，集中型同一性的企业文化有助于组织的协调统一，从而有利于培养完善的执行能力；另一方面，企业文化尤其是核心价值观直接影响企业的战略意图，从而影响了在企业能力体系中对于潜在核心能力的选择和最终形成。

METHODS: Human PDLC cultured in vitro were collected and seeded on three-dimensional framework of coral and coralline hydroxyapatite.

组织块法培养人PDLC ，传代扩增后，接种于珊瑚和珊瑚转化羟基磷灰石两种三维支架上，体外继续培养 3d ，进行细胞计数和扫描电镜观察。

Objective : To explore the possibility of culturing Adipose tissue derived stromalcells in large quantity by microcarrier suspension system and the multidifferentiationability of the cultured cells.

目的：观察在旋转反应生物器重微载体悬浮培养系统对成人脂肪基质干细胞的培养效果，为大量、快速扩增组织工程种子细胞提供实验依据。

In specialized diaphysis course teaching does guidance with the teaching theory from correcting the relation of the teaching subject and object to set out,and selects heuristic teaching method.

在专业骨干课教学中，从端正教学主体客体出发，以教学理论为指导，采取启发式教学方式，在教学内容的组织过程中及时补充科研动态，拓宽教学知识面的同时突出专业重点，重视对学生专业实践能力的培养，并适应园林专业的新发展，教师作为传授专业知识的载体，应加强专业知识的积累与更新，以保证教学良性循环，培养社会和时代发展需要的专业人才。

Preadipocytes from epiploon of newborn calf were cultured by monolayer adherent method, and total RNA of the preadipocytes was extracted on day 4,6,8,10,12,14 and 16 post-culture, respec-tively. The abundance of ADPN mRNA and HSL mRNA was detected by real-time fluorescent quanti-tative RT-PCR during differentiation period from preadipocyte to adipocyte.

选取犊牛大网膜脂肪组织，进行牛前脂肪细胞的单层贴壁培养，在细胞接种后第4、6、8、10、12、14、16d分别提取细胞总RNA，采用实时荧光定量RT-PCR方法，检测了体外培养的犊牛前脂肪细胞不同时期ADPN基因mRNA和HSL基因mRNA的表达情况。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。