组织培养

Through a study on tissue cultureof the bud scale of corm of Lili longiflorum separated from the plant ,several practical TC prompt proliferation techniques ,such as inducing of lush bud ,proliferation cultivation of successive generation, cultivation of growing roots and transplanting , etc., are researched in this paper .

通过对百合球茎鳞片离体组织培养的研究，探讨了丛生芽诱导、继代增殖培养、生根培养及移栽等方面的组培快速繁殖以及生产上实用的组培技术。

The results showed that the number of chromosome, which was from the cultured seedlings of meristem tip and the rank seedlings of meristem tip, was not altered, both the number were 2n=16,the hereditary stability of callus and regeneration plant was poor; and the variation percentage of chromosome in callus was 43.4%, among which haploid accounted for 6.7%, triploid accounted for 2.5%,tetraploid accounted for 10%,pentaploid accounted for 4.2%,hexaploid accounted for 3.3%,septuploid accounted for 4.2%,octoploid accounted for 3.3%,dysploid accounted for 9.2%;the chromosome variation percentage of differentiation seedlings derived from callus was11.7%,among which haploid was 6.7%,triploid accounted for 1.7%,tetraploid was 3.3%.

结果表明，茎尖分生组织培养的幼苗及丛生苗遗传稳定，其染色体未发生倍性变异，均为2n=16；愈伤组织及其再生苗遗传稳定性较差，愈伤组织染色体数变异率为43.4%，其中单倍体占6.7%、三倍体占2.5%、四倍体占10%、五倍体占4.2%、六倍体占3.3%、七倍体占4.2%、八倍体占3.3%、非整倍体占9.2%；愈伤组织分化苗染色体变异率为11.7%，其中单倍体占6.7%，三倍体占1.7%，四倍体占3.3%。

Result The optimum medium formula for protocorm propagation in tissue culture of C. grandiflorium was MS+0.1 mg/L 6-BA. The suitable medium for protocorm induction and differentiation in tissue culture of C. grandiflorum was WS+1.5 mg/L 6-BA+0.3 mg/L NAA with the bud-inducing rate up to 130%. The suitable medium for root inducement was 1/2MS+0.2 mg/L NAA+1.0 mg/6-BA and the survival rate of in vitro plantlets after transplanting was 78%.

结果］大花蕙兰组织培养原球茎粉殖以MS+0.1 mg/L NAA+2.0 mg/L6-BA培养基配方最好；适合大花蕙兰组织培养原球茎诱导分化的培养基配方为MS+1.5 mg/L6-BA+0.3 mg/L NAA，诱导出芽率可达130%；适合大花蕙兰诱导生根的培养基配方为1/2 MS+0.2 mg/L NAA+1.0 mg/L6-BA，试管苗移栽后成活率为78%。

The stem apex tissue culture of Zingiber officinale Rosc. and the control of contamination rate were studied in the experiment.

以生姜茎尖为外植体，筛选了愈伤组织和芽分化的最适培养基和培养方式，并探讨了生姜组织培养过程中污染率的控制方法。

Based on taking the orthogonaldesign experiments and by using the technique of plant tissue culture in this paper, theexperiment-system of tissue culture and rapid propagation of Mozzie buster andOcimum basilicum have been successfully established.

以MS为基本培养基，运用正交设计方法，利用植物组织、细胞培养技术，成功地建立了驱蚊草和罗勒的组织培养快繁技术体系。

Based on taking the orthogonal design experiments and by using the technique of plant tissue culture, the experiment-system of tissue culture and rapid propagation of Mozzie buster was successfully established.

运用正交设计方法，利用植物组织、细胞培养技术，成功地建立了驱蚊草组织培养快繁技术体系。研究出：不定芽诱导的最适培养基为MS+6-BA0.2mg/L+NAA0.3mg/L，增殖倍数为6.73；生根培养基为1/2MS+NAA0.4mg/L，平均每株生根数为11.2条，生根率为90.0%。

At the same time, the extract antifungal activity of wild, cultivation and tissue cultivated Xanthium sibiricum were comparatively researched in five testing fungus by the growth rate method and cucumber cotyledon method.

以苍耳叶片、叶柄及茎段为外植体，在MS附加不同激素种类和浓度配比的培养基上对苍耳愈伤组织进行诱导培养，并对获得生长迅速、性状好的愈伤组织培养条件及培养基进行了系统研究。

On the basis of investigating the resource and utilization of Iris plants in Nanjing, Hangzhou and Wuxi, the paper selected five plants: Iris pseudacorus、 Iris sanguinea、 Iris kaempferi、 Iris japonica、 Iris tectorum to find their tissue culture technology and shade tolerance with lots experiments. The results were summarized as follows:In the culture of Iris sanguined, The shooting tip in the spring and the medium supplemented with 6-BA(1mg/L)+NAA(0.2mg/L) testified the best grouping.

本文在对南京、杭州、无锡等地鸢尾属植物及其园林应用实地调查的基础上，以黄菖蒲、溪荪、花菖蒲、蝴蝶花、鸢尾等五个种为实验材料，从其部分种的组织培养技术和耐荫性两方面入手，通过大量的实验并对所得数据进行统计分析，得出以下结论：溪荪的组织培养中，以春季萌发的茎尖为外植体，以MS培养基附加低浓度的6-BA(1mg／L)与NAA(0.2mg/L)为最佳的诱导分化的组合。

Endothelial Cells ;; Tissue Plasminogen Activator ;; Tissue Culture Techniques ;; Brain ;; Mice ;; Stroke ;; Cell Culture Techniques ;; In Vitro

内皮细胞；；组织型纤溶酶原激活物；；组织培养技术；；脑；；小鼠；；卒中；；细胞培养技术；；体外研究

The asexual culture of American black walnut was studied by tissue culture.

采用组织培养方法对美国黑核桃进行组织培养研究。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。