组织培养

Allantois and surrounding tissues of 8.5 day post coitum embryos, the hindhut and surrounding tissues of 9.5 dpc and 10.5 dpc embryos, the gonadal ridges and surrounding tissues of 11.5 dpc and 12.5 dpc embryos were collected and digested with 0.25%pancreatin-0.02%EDTA, then the cells were cultured in the plastic petridishes which are pretreated with 0.1% gelatine.

实验方法：取胚龄8.5 d胎鼠尾端尿囊及周围组织，取胚龄9.5~10.5 d胎鼠后肠及周围组织，取胚龄11.5 d，12.5 d胎鼠生殖嵴及周围组织，分别经0.25%胰酶-0.02%EDTA消化并接种于0.1%明胶包被的培养皿中。

Structures of the tissue-engineered skin constructed for 2 weeks were similar to those of normal skin except that there was no corneum in the tissue-engineered skin.

组织学观察发现，体外培养2周的组织工程皮肤与正常皮肤的组织结构特征相似，仅缺少角质层。

No callus could be induced from leaf or petiole explants of varieties F30 and Ⅱ cultured on MS medium if supplemented alone with 6-bezyladenine （6-BA） or thidiazuron, or kinetin. However, the calli were induced on MS medium containing 2.0 mg L-1 6-BA and 0.5 mg L-1 NAA （α-naphthaleneacetic acid）, and the calli derived from petioles of variety Ⅱ could produce redifferentiated shoots, but of variety F30 could not. The later could only produce shoots directly from the base of the petioles.

将品种F30和Ⅱ的叶片和带叶叶柄接种到含不同浓度单一细胞分裂素（6-BA、TDZ、KT）的MS培养基上培养，均未能诱导出愈伤组织，而在含2.0mgL-1 6-BA＋0.5mgL-1 NAA的培养基上可以诱导出愈伤组织，且由品种Ⅱ叶柄诱导产生的愈伤组织可再分化出芽，品种F30诱导的愈伤组织却不能分化，但在其叶柄基部可直接出芽。

Given dividing campus culture into two kinds of cultures, namely, the academic culture and the nonacademic culture, the author discussed their medium function in affecting college students' quality respectively, coneluded the affecting mechanisms of campus culture and their cooperation characteristic the closed process mechanism and the open adjustment mechanism.

大学校园文化通过发挥内部教育功能，从意义表达和具体操作两个功能层面，直接与大学生素质培养活动相联系，影响大学生的培养和发展。 8、大学校园文化影响大学生素质的培养和发展，以大学办学思想为核心、大学教师文化主体为重要载体、通过学术性与非学术性文化活动两大基本途径，具有它自身的组织与运行原理；形成影响大学生素质的闭合过程机制和开合的调谐机制。

Fig.1 SHEE cultured on coverslide, the living cells were growing in single layer with rich cytoplasm, the nuclei were uniform in size with a nucleolus ph ×400 Fig.2 SHEE had a nucleus with ellipse shape, large nucleolus and the cytoplasm contained mitochondria and tonofibrilEM ×10 000 Fig.3 SHEE was spherical in shape, with pseudopods attached on petri dish and abundant villi on cell surface SEM ×5 000 Fig.4 Same as in Fig.3, cell attached on petri dish, appeared stellate or polygonal in shape, with abundant pseudopods and cytoplasmic processes. Protrusive nuclear region in central part of the cell had more micro-villi SEM ×5 000 Fig.5 Chromosomes of SHEE cells belonged to diploidy type Giemsa ×1 000 Fig.6 The SHEE cells of stained in dark brown by Ki67 immunohistochemistry were the proliferative cells Immunohistochemistry ×400 Fig.7 In SHEE cell culture, the nucleus stained red or pink by PI was dead cell, the green nucleus was living cell Fluorescent ×400 Fig.8 The cell labeled by TdT was apoptotic cell in which the chromatin of nucleus condensed in block, a pyknotic nucleus in the upper right conner was seen TdT labeled ×400

图1 SHEE培养在盖坡片上，活细胞单层生长，胞浆较丰富，细胞核大小一致，有核仁×400 图2 SHEE培养细胞细胞核椭圆形，核仁较大，胞浆有较丰富的线粒体和张力原纤维EM ×10 000 图3 SHEE细胞呈球状，有伪足贴壁，表面有密集微绒毛SEM ×5 000 图4 同上细胞贴壁，呈星状或多角形，有丰富伪足和胞浆突，核区隆起有较多微绒毛SEM ×5 000 图5 SHEE细胞染色体仍属二倍体Giemsa染色×1 000 图6 SHEE细胞Ki67免疫组织化学染棕黄色为增殖细胞×400 图7 SHEE培养细胞出现死细胞，胞核和胞浆PI染色呈红色或淡红色，蓝色细胞核为活细胞荧光显微镜×400 图8 细胞TdT标记阳性为凋亡细胞，染色质凝集呈块状，右上角有一固缩细胞核TdT标记×400

Activity of alkaline phosphatase and ability of formation for calcifying nodules were determined.

抽取健康成人骨髓组织，用Percoll分离液分离出骨髓中的单个核细胞，在含体积分数为10%小牛血清的高糖DMEM培养液中，置于37℃、含体积分数为5%的CO2湿化空气孵箱中培养，通过传代培养扩增骨髓基质干细胞，传三代时改用含地塞米松、β-甘油磷酸和维生素C的条件培养基培养，用倒置显微镜、HE染色观察增殖和分化情况，并测定碱性磷酸酶活性和钙结节形成能力。

This article embarks from my school actual situation, elaborated from five aspects raises the student creative thought the necessity and the possibility, the union concrete teaching practice, take applies the heuristic teaching to build the creative thought scene as the student, in the classroom in striation pays great attention to stimulates student"s seeking knowledge desire, lights student"s creative thought desire fire, fully displays the historical data the function, takes to accumulate, the collection source material, the organization discusses, encourages to question, excavates the student latent creation consciousness, raises aspect and so on migration ability which student"s contrast inference extrapolates has carried on the exploration, has yielded the trifle result on the student body, simultaneously to explored the question which discovered to carry on reconsidering, Realized to raises student"s creative thought by no means merit of the first, needs the historical teacher to persevere, relentlessly diligently, also needs unceasingly to summarize, reconsidering, the enhancement in the practice.

本文从我校的实际情况出发，从五个方面阐述了培养学生创造性思维的必要性和可能性，结合具体教学实践，以应用启发式教学为学生营造创造性思维情景；课堂教学中注重激发学生的求知欲望，点燃学生的创造性思维的欲火；充分发挥史料的作用；重视积累、收集素材，组织讨论；鼓励质疑，挖掘学生潜在的创造意识；培养学生的对比推理举一反三的迁移能力等方面进行了探索，在学生身上取得了些许成果。同时对探索中发现的问题进行了反思，认识到培养学生的创造性思维并非一日之功，需要历史教师持之以恒，坚持不懈地努力，也需要在实践中不断总结、反思、提高。

Furthermore there are more apoptosis cells in inner root sheath than in outer root sheath. Conclusion When we culture human hair follicle in vitro, Williams E serum-free medium is a suitable choice.This model is a fine model also, which is used for screening drug that may accelerate or inhibit the hair growth.Cryo-section can simplify procedure of paraffin section in histology detection of culture human hair follicle in vitro.We can observe the ultrastructural change of human hair follicle in vitro by transmission electron microscope,which can assist to observe apoptosis cells. The eyepiece micrometer is a simple tool for measuring length of hair follicle in vitro.

在离体培养人头皮毛囊时，Williams E无血清培养基是合适的选择，该模型也是筛选促进或抑制毛发生长药物的良好模型；离体培养毛囊组织学检测用冰冻切片可以简化步骤；透射电镜可以用来观察离体培养的毛囊的形态和超微结构的变化，也可以用来协助观察细胞凋亡的状况。

Honghezi,Dongbi,Shieryuelongyan,etc.The results showed that the method of Calcium-alginate -embedded suspended culture shaking at50r/min was suitable for longan proto plast culture;and the optimal regeneration,with up to5%of microcolony formation,occurred when protoplasts from suspensions were cultured ...

研究结果表明，海藻酸钙包埋悬浮振荡培养(50r/min)是龙眼悬浮细胞原生质体培养的适宜培养方式，在含有多种生长调节剂的改良MS培养基(MP6)上，再生小克隆的形成频率可高达5%；采用龙眼胚性愈伤组织体胚诱导、成熟和萌发的优化方案，原生质体再生小克隆分化子叶形胚状体的频率达100%，萌发植株的频率一般大于45%。

In this essay, the culture methods of corneal epithelial cell and keratocytes have been studied. The method of rabbit corneal epithelial cells has been improved in order to solve the problem that the cells moved slowly and could not form monolayer when the traditional tissue nubbles cultivation was adopted.

本文对兔角膜上皮细胞和基质细胞的培养方法进行了研究，解决了传统角膜上皮细胞组织块贴壁培养时细胞迁出慢，不易形成单层的难题，建立了兔角膜上皮细胞原代培养的方法。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。