组织培养

objective to investigate the influence of dit on the proliferation of human thyrocytes and compare dit with ki.to study a good kind of supplement to usi that was safe and effective.methods the human thyroid epithelium cells from para-adenoma normal tissues from thyroid adenoma patients were cultured in the absence or presence of 10-8～10-3mol/l dit or ki.mtt method was employed to obtain the proliferation of cultured human thyrocytes.

取甲状腺腺瘤旁正常甲状腺组织，应用原代细胞培养技术进行细胞培养，以不同浓度(10-8～10-3mol/l)的dit和ki刺激单层培养的甲状腺细胞，采用四甲基偶氮噻唑蓝法测定各组甲状腺细胞的增殖情况。

The results showed as follows: 3mg/LBA in differentiation medium was favorable for regeneration of callus; addition of 3mg/LABA, sucrose substituted with maltose, or sucrose partially substituted with mannitol in subculture media improved the status of callus in a degree and enhanced the differentiation frequency; partial desiccation treatment significantly increased the regeneration frequency of callus; addition of AgNO3 or CuSO4 in differentiation medium contributed to plant regeneration , The two resistant calli and fifteen regenerated plants were obtained when the calli of mature embryo of 7001S were infected with Agrobacterium carrying bar and CPTI gene.

结果表明：3mg/LBA对分化有利；继代培养基中加入3mg/LABA，以麦芽糖代替蔗糖，或以甘露醇代替部分蔗糖在一定程度上可以改善愈伤组织状态，提高分化率；干燥处理能明显提高愈伤组织分化率；分化培养中添加一定浓度的AgNO3和CuSO4有利于愈伤组织分化。借助农杆菌介导bar基因和CPTI基因对水稻进行遗传转化，获得2块抗性愈伤组织和15棵再生苗。

The results showed as follows 3mgLBA in differentiation medium was favorable for regeneration of callus; addition of 3mgLABA, sucrose substituted with maltose, or sucrose partially substituted with mannitol in subculture media improved the status of callus in a degree and enhanced the differentiation frequency; partial desiccation treatment significantly increased the regeneration frequency of callus; addition of AgNO3 or CuSO4 in differentiation medium contributed to plant regeneration , The two resistant calli and fifteen regenerated plants were obtained when the calli of mature embryo of 7001S were infected with Agrobacterium carrying bar and CPTI gene.

结果表明：3mgLBA对分化有利；继代培养基中加入3mgLABA，以麦芽糖代替蔗糖，或以甘露醇代替部分蔗糖在一定程度上可以改善愈伤组织状态，提高分化率；干燥处理能明显提高愈伤组织分化率；分化培养中添加一定浓度的AgNO3和CuSO4有利于愈伤组织分化。借助农杆菌介导bar基因和CPTI基因对水稻进行遗传转化，获得2块抗性愈伤组织和15棵再生苗。

Methods In this study,a plasmid vector,rAd5,and rAAV2 were used to transfect rat cochlear marginal cells of stria vascularis by injection into the perilymph through the round window membrane,and the transfection efficiency, target tissue accessibility, cell/ tissue toxicity, time course of expression, and effect on hearing were evaluated in vivo and in vitro.

结果三种载体对原代培养的大鼠耳边缘细胞的转染研究发现，腺病毒的转染效率最高，而腺相关病毒转染对细胞活性影响最小。rAAV2和rAd5对大鼠耳蜗组织的感染实验发现，腺病毒和腺相关病毒携带的增强型绿色荧光蛋白可在多种耳蜗组织中及转染对侧耳蜗组织表达，且并不引起明显的耳蜗组织细胞凋亡。rAAV2携带的EGFP基因在第90天时仍可检测到大量表达。rAd5携带的EGFP基因在第30天时表达明显减弱。

The analysis shows:(1) The elementary targets of the Sanda courses should focus on forming the students\' interest in the Sanda courses;on the basis of mastering the Sanda basic etiquette criteria and Sanda basic techniques in common use,the courses should basically form the character of confidence,bravery and pertinacity and at the same time promote the physical health,form the competition consciousness,cooperation spirit, respect for the teacher and the concept of collectivity and discipline.

分析表明：(1)散打课程初级目标应注重培养学生对散打课程的兴趣，在掌握散打基本礼仪规范和常用基本技术的基础上，初步培养自信心和勇敢顽强的意志品质，同时促进身体健康，培养竞争意识、合作精神、尊师重道的良好意识以及较强的组织纪律性。

In inducing callus process, the suitable illumination conditions were the former period with darkness culture and the later period with light culture. In inducing dedifferentiation and redifferentiation process, the temperature of 24-26℃ was suitable.

在愈伤组织诱导时，光照条件以前期暗培养、后期光培养为宜，在诱导脱分化和再分化时，培养条件以温度24-26℃为宜。

Methods We focused on the cultivation of249nurse students in t wo respects:one is culti-vation of psychological quality including rigorous dem ands,strong-mindedness,open-mindedness,increase of the ability to face in-tense pressure;the other the cultivation of self-study ability including understandin g the organic connection among the learned sub...

方法重点对两个年级249名护生加强心理素质的培养，包括严格要求，培养坚强意志，开阔胸怀自我疏导，提高面对压力的应对能力；自学能力的培养，包括学会各学科知识的有机联系，学会抓纲、提炼、概括以及组织课外自学。

Chinese is an important basal and tool subject of preschool education. It is necessary for training teachers of preschool education with more skills and knowledge.

语文课作为幼师教育的一门重要的基础课和工具课，对于培养一专多能的幼儿教师具有其他课程不能替代的作用，它不仅传授基本的文化知识，培养学生倾听、口头书面表达、朗读等诸多技能，而且对学生人生观、价值观、儿童观、教育观的形成以及组织活动、动手操作、研究行为的培养、继续学习的能力等有很大的影响。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

The normal and abnormal embryoid were distinguished by the rate of normal plantlets induced and the regeneration modes of the cottons varieties were compared to two ways, one with the highest rate of normal inducement was the normal way of regeneration plantlet from callus, embryogenetic callus, global embryoid, heart-shape embryoid, torpedo-shape embryoid, cotyledon embryoid to normal plantlet, etc, the other with the lowest rate of normal inducement was the abnormal way from callus, embryoid callus, global embryoid-like, heart-shape embryoid-like, fish torpedo-shape embryoid-like, cotyledon embryoid-like, abnormal plantlet to normal plantlet, etc.

转化后采用平行实验得到不同品种的不同培养体系的愈伤组织、胚性愈伤组织、胚状体、类胚状体、畸形苗和正常苗，以最终正常苗诱导率高的体系的材料作为正常苗的发生过程，以诱导率低的作为畸形苗的发生过程，即分为正常苗的发生过程，愈伤组织——球型胚状体——心型胚状体——鱼雷型胚状体阶段——子叶型胚状体——正常苗：畸形茁的发生过程，愈伤组织——类球型胚状体——类心型胚状体——类鱼雷型胚状体阶段——类子叶型胚状体——畸形苗。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。