组织培养

Use constituent piece to stick mural way depart to foster Niu Er to become fiber cell above all, after classics generation is rarefied, fat simple way will be contained double the skin of the 4th acting ox that outside conveying carrier to turn into body, the particularity of person bacteriolysis enzymatic mammary gland that chooses number fosters becomes fiber cell, g/mLG418 chooses 500 μ 2 weeks, g418 halve continues to develop 3 generation of 2 ～, catch the cell after choosing to undertake PCR detects to turning next reach nucleus analysis.

首先采用组织块贴壁法分离培养牛耳成纤维细胞，经传代纯化后，脂质法将含有双重筛选标记的人溶菌酶乳腺特异性表达载体转入体外培养的第4代牛皮肤成纤维细胞，500μg/mLG418筛选2周，G418减半继续培养2～3代，然后对转染筛选后的细胞进行PCR检测及核型分析。

Radiography and double fluorochrome labeling were taken. Dogs were sacrifice at 1, 3, 6 months post operation and image analysis were done on undemineralized slices then subject to statistical analysis by SAS software, Version 6. 21. 3. BMSCs in vitro induced and proliferated through complete blood culturing were seeded into tricalcium phosphate , fdDBM and 2 kinds of coralline hydroxyapatite with 200μm and 500μm porosity (C200 and C500) respectively.

将全血培养法体外诱导、扩增培养的BMSCs分别与三磷酸钙(tricalcium phosphate，TCP)、两种孔径的珊瑚羟磷灰石（200μm，500μm）以及制备的fdDBM复合，体外培养4～7日后，进行扫描电镜、组织学观察，并行裸鼠皮下回植，了解包括空白DBM材料在内的5组样本的异位成骨情况，经IMAGER-PRO PLUS软件进行图像分析，并进一步行统计分析。

This process aims at recapitulating the normal embryologic development of the organ of interest. The main difference between in vivo technology and in vitro technology is: in vitro technology emphasis on in vitro cell culture. It has special value for the specific tissue such as articular cartilage.

体内组织工程技术与体外组织工程技术的目标相同，都是实现组织完全再生；区别在于其达到再生的方法：体外组织工程技术强调细胞的体外培养，并使这些细胞产生功能，这对有些组织有特殊价值，如软骨。

In vitro, bovine mammary epithelial cells were isolated and cultured by the tissue explant method in order to investigate the optimal culture conditions.

方法］采用组织块法，在体外进行乳腺上皮细胞的分离培养，探索其在生化培养箱中合适的培养条件。

There has some disadvantages using these methods, including scarcity of donor site, keratinization and hair growth in transplanted skin, donor site morbidity.

组织工程为口腔粘膜缺损修复开辟了新途径，即应用人体少量的组织细胞经体外培养扩增后接种在支架材料上，移植到组织缺损处恢复组织的完整性。

B Of the six basic media MS, MS1/2 (half-strength of MS salts and vitamins), WPM, DKW, B5 and SH, MS1/2 was the most proper one to induce somatic embryos. Somatic embryos generally regenerated directly from excised zygotic cotyledons. PGRs combination affected somatic embryogenesis significantly. Medium with NAA 1mg/L, TDZ 0.05mg/L, IBA 2—10mg/L combined with BA 10mg/L, or IBA 10mg/L integrated with BA 0-2mg/L gave the highest induction rate. Excised zygotic hypocotyls had the strongest potential to produce callus. Callus induction was also affected significantly by media and PGRs. The proper callus induction condition was MS1/2 medium containing NAA 1mg/L, IBA 10mg/L, BA 2-5mg/L and TDZ 0.05mg/L. Harvest period affect somatic embryogenesis significantly. Zygotic embryo explants collected from the end of July to the middle of August had strong potential to generate somatic embryos, when endosperm finished solidification, different parts of the embryos were completely formed, the size of embryos occupied about 2/3 of the embryo sac. Provided with optimized conditions, direct somatic embryogenesis rate can attain to 33. 68%, and callus induction rate of hypocotyls was up to 90.7%. Cytological observation on megasporogenesis and zygotic embryogenesis of Manchurian ash showed that the ovary was twicarpellum, twilocular with two ovules each loculus. The ovule was tenuinucellar and anatropous, with one megasporcocyte. The development of embryo sac is of the Polygoum type.

体细胞胚胎发生研究的结果表明：(1)成熟过程中的合子胚是诱导水曲柳体细胞胚胎发生的最佳外植体材料；(2)在所试验到的MS、MS1/2（将MS的所有成分均减半）、WPM、DKW、B〓、SH等六种基本培养基中，MS1/2是最适合诱导水曲柳体细胞胚胎发生的基本培养基；(3)水曲柳的体细胞胚胎发生以直接发生为主，体细胞胚主要来自于从合子胚分离的完整子叶；(4)培养基中的激素组合对水曲柳的体细胞胚胎发生有显著影响，诱导直接体细胞胚发生较好的激素组合有NAA 1mg/L+IBA 2，5，10mg/L+BA 10mg/L+TDZ 0.05mg/L和NAA 1mg/L+IBA 10mg/L+BA 0，2mg/L+TDZ 0.05mg/L；(5)合子胚分离的下胚轴具有最强的愈伤组织诱导潜力，少数愈伤组织可以分化出体细胞胚；(6)愈伤组织的诱导也受培养基和激素配比的显著影响，最适宜诱导的培养条件为MS1/2+NAA 1mg/L+IBA 10mg/L+BA 2，5mg/L+TDZ0.05mg/L；(7)采种时间对体细胞胚胎发生有显著影响。7月末到8月中旬的合子胚具有较强的体细胞胚发生潜力，此时种子尚未成熟，胚乳已呈固态，种胚的各个部分已分化完全，种胚体积占胚腔的大约2/3；(8)在各自综合的最适条件下，完整子叶的体细胞胚诱导率可达33.68%，下胚轴的愈伤组织诱导率可达90.7%。

The result indicated:① In accordance with culturing in the medium containing MS, SH, N6 macroelement, respectively, the effect of MS or SH macroelement in the primary culture is better than that of N6 macroelement, and the effect based on N6 macroelement is the best from 5th to 9th subculture.

结果表明：①在含MS、SH、N6的大量元素的培养基上的初代培养，MS或SH的效果好于N6，愈伤组织第5~9次继代培养中，N6大量元素培养基的继代培养效果最好。

College of Animal Sciences, Zhejiang University , Hangzhou 310029, China Abstract: The mammary tissue from dairy cows was cultured by the roller tube culture and stationary culture (24 well plastic cul-ture plates).

以液氮冷冻泌乳奶牛乳腺组织为研究材料，对奶牛乳腺组织的旋转培养法与贴壁培养法进行了比较研究。

Train it under the condition in 23-25 degrees Centigrade, The monoploid that the rice anther train Callus to "zhonghua11 ", than maturity liploid that embryo lead of it,s callus grow speed to be one times faster and about.

在23~25℃培养条件下，&中花11&水稻花药培养的单倍体愈伤组织，比其成熟胚诱导的二倍体愈伤组织生长速率快一倍左右。

Methods: BALB/c mice were intranasally infected with respiratory syncytal virus before or after sensitization to Japanese Cedar Pollen crude extract. Single-cell suspensions obtained from the lung parenchyma of inoculated mice were re-stimulated in vitro by co-cultivating with UV-RSV or JCP for 48 hr.

用花粉抽提物JCP致敏BALB/c鼠，致敏前或后经鼻感染RSV，提取肺组织细胞，体外培养，ELISA试剂盒检测培养上清中细胞因子水平；姬姆萨染色法分类并计数肺组织内浸润细胞数量；ELISA方法检测血清特异性抗体IgE水平。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。