组织培养

In vitro, we cultured human and rabbit retinal Müller cells by way of mixed enzyme digestion and tissue adherence, using human retina of an abortus of 6 months and healthy rabbit.

取孕6个月引产胎儿，死后2h内无菌条件下摘取眼球，剥取视网膜组织，混合酶消化法培养人视网膜Müller细胞；取健康新西兰大白兔，死后以最快的速度无菌条件下摘取眼球，组织块贴壁法培养兔视网膜Müller细胞；利用含胎牛血清的高糖DMEM作为培养液，不添加生长因子。

This paper studied the effects of types of medium, conditions of culture, different kinds of carbon and nitrogen sources and plant growth hormone in medium component on Agave sisalana tissue and cell culture for inducement of Agavain—SH.

本论文研究了剑麻组织和细胞培养中培养基类型、培养条件、培养基组成中不同碳源、氮源和激素对愈伤组织细胞生长和蛋白酶活力的影响；。。。

Objective To verify the application of anterior segment perfusion culture and trabecular meshwork organ culture for glaucoma study.

目的 了解猪眼小梁组织的灌流眼前节培养和组织块培养在青光眼研究中的应用。

This dissertation theoretically demonstrates the feasibility to achieve constant direct current-stimulation-circuitry under microampere , bring forward design scheme software and hardware system using in cellular electrical stimulation. In order to further quantitatively research on direct current-stimulation and ascertain the best intensity and density and stimulative current , the quantitative and qualitative analyse for gained diagnostic data are also been discussed in this paper.

本文正是从该理论基础出发，论证了实现微安级直流电刺激的离体组织细胞生长培养实验系统的可行性，提出了用于刺激实验的软硬件系统的开发放案，并对采集数据的定量和定性的分析作了初步的探讨，希望通过实验能够初步确定适合该组织细胞生长培养的最佳刺激电流和最佳刺激时间。

Calli of Anthurium andraeanum 'Jolanba'derived from leaves of in vitro plantlets were cultured with three different treatments to observe their redifferentiation behaviors , and their physiological and biochemical features are discussed. The results went as followed.

对花烛 'Jolanba'无菌苗叶片诱导产生的愈伤组织进行3 种不同的培养处理，观察其再分化表现，并探讨了3 种继代培养愈伤组织的生理生化特点。

In this paper, we studied M. meretrix ferritin, cathepsin B and caspase genes, which are involved in clam larval shell formation, nutrition, metabolism and apoptosis, respectively. We have cloned the three genes, investigated the temporal and spatial expression profile both at gene and protein level in trochophore (L1), D-veliger (L2), pediveliger (L3) and postlarvae (L4). The potential roles of these proteins were analyzed with specific inhibitors during larval development. Firstly, embryos were found developed into trochophore-like larvae with no shell if cultured at gastrula stage in artificial seawater without iron. Shell-like structures were formed only in the presence of iron. The larvae which had been transferred at L1 stage into ASW developed normal shell. This indicated that iron and iron associated protein are important for larval shell formation. The EST sequence which is homologous with ferritin, which is a principal iron metabolic protein, was selected from the M. meretrix cDNA library. The full-length of ferritin subunit cDNA was cloned by RACE. The results of real-time PCR revealed that the MmeFer mRNA expression changed before and after the larval shell formation.

本论文以文蛤幼虫为研究对象，分别对文蛤幼虫发育过程中贝壳形成相关的铁蛋白、营养及变态相关的组织蛋白酶B及变态过程中细胞凋亡相关的caspase三个基因进行了克隆，分析了基因及编码蛋白在担轮幼虫期(L1)、D形幼虫期(L2)、壳顶幼虫期(L3)和稚贝期(L4)的时空表达特征，解析了其可能的功能，并研究了相应酶类的特异性抑制剂作用对幼虫发育过程的影响，进行了目标蛋白的功能验证，详述如下：研究结果显示，在文蛤胚胎发育到原肠胚时放入不含铁离子的人工海水中培养，发育成无壳的畸形，随着人工海水中铁离子添加浓度的升高，幼虫长出壳状组织接近正常状态；而发育到L1期幼虫放入不含铁离子的人工海水中培养却可以发育出正常的壳，推测铁和铁代谢相关蛋白在幼虫贝壳初始形成有重要的作用。

To obtain more messages from Ardisia, some studies, such as characters of seeds germination and storage, factors influencing callus induction and growth, monitoring bergenin in callus of Ardisia spp and plant regeneration of Ardisia spp, were carried out. As a result, calli were obtained from nine species of Ardisia and bergenin, the major pharmaceutical element of Ardisia, was proved to exist in six of them when checked by HPLC. Plant regeneration systems were established from three species of Ardisia too. These works are all reported for the first time.

为更好地利用该属植物资源，本文对紫金牛属植物的种子萌发和贮藏，愈伤组织的诱导和培养，培养物中岩白菜素含量的检测，植株再生等问题进行了研究，首次成功地获得了紫金牛属9个种的愈伤组织并在其中6个种中检测到主要药用成分岩白菜素的存在，同时首次建立了该属3个种的再生体系。

The integrative ability, organizing ability, communicative ability of trainings was deficiency. Fourthly, the power of social physical educators in Guangxi universities was so weak that it couldn't satisfy the need of specialty development. For example, the number of special teacher and the practicing ability of teachers are deficiency. The teachers that master special theoretic well and have practicing experience are deficiency especially. Fifthly, social physical practice base have not been building in Guangxi universities. And the necessary equipments of gymnasium are deficiency at the same time. Sixthly, the whole sports technology levels of new social physical students are low and hard to fit for social needing. Seventhly, the professional ideology of students is not stable enough. For example, the special concept and cognition of students are dim. The attitude for learning is not right. The most of students want to or have trend to change their profession after graduating. Eighthly, the ability of students fostered by social physical education in various Guangxi universities has a gap with the ability that students should hold for adapting the need of Guangxi employer. The special technical levels of social physical students are weak. Ninthly, in investigation of social physical personnel being demanded by Guangxi area employers, we found that few social physical personnels were demanded by business unit, the state-operated factory and mining enterprise because of being influenced by economical environment. And the more social physical personnels were demanded by social physical directors, fitness centers and each kind of sports association and so on.

这主要表现为：不够科学的课程体系，在实践性教学环节中的重视程度不够，对学生的综合能力、组织管理能力、社会交际能力的培养较为欠缺；4、对社会体育专业的发展需求这一点而言，广西各高校该专业的师资力量还略显薄弱，难以满足社会的需求，主要体现在：缺乏足够的专业教师数量、教师实践能力欠强，尤其缺乏既有较高理论素养又有实践经验的教师；5、社会体育专业的实习基地在广西各高校中都尚未建立起来，同时一些必备的体育场馆的设施也较为缺乏；6、由于入学时广西社会体育专业的学生在运动技术、技能整体水平相对较低，因此在一些专业方向的需求上难以达到满足；7、在专业思想这一方面而言，广西各高校社会体育专业的学生显得不够稳定，主要表现为：淡薄的学生专业观念，模糊的专业认识，不够端正的学习态度，甚至有相当一部分学生准备毕业后改行或有改行倾向；8、广西各高校社会体育专业培养学生目前所具备的能力与当前广西用人单位所需求的社会体育专业人才应具备的能力尚有一定差距，主要体现在：目前社会体育专业学生的专业技术水平较低，组织能力和社会交际能力较为欠缺等。9、在广西地区用人单位对社会体育专业人才需求量的调查中发现：大多数的企事业单位和国营厂矿企业因受到经济大环境的影响，基本上都不设置专职的体育管理人员，对社会体育专门人才的需求量较少，需求量较大的还是社区体育指导员、体育健身中心、各种体育协会等。

Immunohistochemical identification of cultured conjunctival cellsWhen the second cultured generation of conjunctival cells close to confluence, the glass coverslips were removed in situ fixation by methanol and DAB color immunohistochemical staining by CK13 monoclonal antibody in time.3. growth curve of conjunctival cellsThe 1st,3rd,5th passage cells were selectecd randomly and subcultured at the density of 5×10~4/ml,after culturing for another 1-9 days, were counted and compared the cell number every day.

采用消化后组织块法培养结膜原代细胞，并进行传代。2、培养结膜细胞的免疫组化鉴定把第2代的结膜细胞接种于置在培养皿中的盖玻片上，当细胞接近融合时，及时取出作原位甲醇固定，CK13单抗、DAB显色免疫组化染色。3、结膜细胞生长曲线的测定随机选取第1、3、5传代细胞，以5×10~4／ml密度传代、分别培养1-9天，每天分别细胞计数并进行分析比较，重复3次。

The hFCECs were cultured by sticking tissues piece method that the cornea were divided into the limbus and central tissue piece and digestive method,respectively.For digestive method,the digestive juice of 5mg/ml DispaseⅡ+0.25%trypsin was chosed.D/F12 with 10%fetal bovine serum and 100 U/ml penicillin and streptomycin were used in this study,the culture condition was 37℃and 5%CO2,and culture medium changed every three days2、Passage of hFCECsAfter more than 80%confluenced,cells from corneal limbus hFCECs were digested for 5-15min with different concentrations of trypsin/EDTA at 37℃,and then passaged at a ratio of 1:2.The cells were subcultured on empty plates,mouse 3T3 fibroblast feeder layer,fetal corneal stromal cell feeder l ayer or HTK feeder layer respectively.And D/F12, mouse 3T3 fibroblast conditioned medium,fetal corneal stromal cells conditioned medium or HTK conditioned medium with 10%FBS and 100 U/ml penicillin and streptomycin were used respectively as the culture medium.And the culture medium were changed every three days as well.

方法一、人胎儿角膜上皮原代和传代培养1、原代培养严格无菌操作获取人胎儿角膜片，采用组织块贴壁法、酶消化法原代培养人胎儿角膜上皮细胞。2、传代培养角膜缘部的细胞生长融合达80%以上，不同稀释浓度的胰酶/EDTA消化液消化传代分别接种于空板、小鼠3T3成纤维细胞饲养层、胎儿角膜基质细胞饲养层及HTK饲养层，培养液分别采用D/F12、小鼠3T3成纤维细胞条件培养液、胎儿角膜基质细胞条件培养液、HTK条件培养液。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。