组织培养

The leaves,pedicel axillary bud,pedicel linternode,stem tip and root tip of butterfly orchid were used as explants,and the best explant and culture medium were screened by testing different culture medium with hormone combination.

以蝴蝶兰的叶片、花梗腋芽、花梗节间、茎尖、根尖为外植体，通过对蝴蝶兰组织培养的不同类型的基本培养基及各种激素配比进行组合试验，筛选出蝴蝶兰组织培养的最适外植体及其培养基配方。

This paper reports something about it .The callus was induced and proliferated by using young stem , leaf and leafstalk as explants .which came out in early spring . In this paper , its effects on induction and proliferation were discussed by using different kinds of explants and hormone and different physical and chemical factors.The growth circle and some physiological and biochemical factors were studied during the culture of callus, a set of rapid propagation techniques were founded, which was rapid, efficient and steady.

本文以丹参初春萌发的幼茎、叶、叶柄为外植体，探讨了不同的外植体、不同的植物生长调节物质、不同的理化因子对愈伤组织的诱导和增殖效应，研究了黑暗和光照条件下愈伤组织细胞培养生长周期以及生长周期中生理生化指标的变化特征，摸索了一套快速、高效、稳定的丹参快速繁殖技术，为丹参组织培养、次生代谢物的生产提供理论依据，为丹参快速繁殖应用于生产提供技术支持。

The authers fetched the embryo calvarial peristeum tissue, got human osteoblast by enzyme-assimilating methods and tissue-block culture methods. We observed the morphological change, growth feature and osteogentic capability, of osteoblast during culture in vitro with phase contrast invert microscope, drew the growth curre and identified the cells by alkaline phosphatase dye. At same time, the morphology and bioactivity of 3-5th-generation osbeoblast and anabiotic cells was studied comparatively. 2. titanium particles were examined by scanning electron and the size was determined by semi-automated image analysis. The 3-5 th gereration of human osteoblast were cultured in medium with different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml). Cell growth and proliferation was detected by MTT method after 2、4、6 days that particles were added into medium and ALP activity was measured by kit after 4、7、10 days respectively. 3. With above same methods,the 3-5th generation of human osteoblasts were cultured for 3、6、9days after different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml) were added into the medium and OPG gene expression was quantified by RT-PCR.

1、取人胚胎颅骨骨膜，采有用酶消化法和组织培养法获取成骨细胞体外培养并传代，观察细胞形态，生物特点及成骨特性，并绘制生长曲线同时碱性磷酸酶染色鉴定成骨细胞以及比较冻存前3-5 代与冻存后成骨细胞的特点。2、电镜下观察钛合金颗粒的形态并测量其粒径，将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞共同培养，分别于第2、4、6 天用MTT 法测量细胞增殖情况及4、7、10天用试剂盒检测碱性磷酸酶活性。3、分别将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞基因培养3、6、9 天用RT-PCR 方法半定量测定骨保护素基因mRNA 的表达。

Methods: Seven male patients with kidney stones were admitted. Abdominal subcutaneous adipose tissue and perirenal adipose tissue were collected from the operating-patients, and were cultivated with different concentrations of curcumin (10 and 100 μg/ml) in vitro.

用体外组织培养法培养手术后离体人脂肪组织，分别予10和100μg/ml姜黄素干预6h和24h，用夹心酶联免疫吸附测定法检测各脂肪组织培养液中APN和IL-6的含量。

Firstly, the limited factors about Zostera marina L.' life in the sea water, the optimal tissue or organ for the tissue culture , the optimal hormone combination and concentration is determined by means of the transplantation of Zostera marina L. and its tissue culture.

首先，通过对大叶藻进行移植，并进行组织培养，来初步探究大叶藻在海水中生活的限制因子以及组织培养的最佳器官、组织和最适激素组合和浓度。

Bioreactor is a kind of device intended for in vitro tissue culture that incubates the cells or tissues taken from living bodies in simulated physiological environment in vivo. On the basis of original cell culture, the present bioreactors apply biomechanical stimulation to simulate the stressed environment of articular cartilage in vivo.

生物反应器是一种体外的组织培养装置，它将活体内取出的细胞或组织在模拟体内生理环境下进行孵育培养使之生长分化，现有的生物反应器主要是在原有细胞培养的基础上加以力学刺激以模拟关节软骨在体内的应力环境。

Based on the results of information investigation, the paper briefly summarizes the research and application of cell engineering technology in agriculture: Chromosome engineering,protoplast culture,anther culture, cell culture, tissue culture and somatic hybridization. The worldwide development and progress in the application of cell engineering in plant breeding are reviewed.

根据文献研究结果，系统地综述了染色体工程技术、原生质体培养、花药培养、细胞培养与无性系筛选、组织培养与体系胞杂交等细胞工程技术，在国内外农作物育种上开发应用后所取得的新进展、新成果，以及这些新成果的产业化，对促进中国农业生产的发展所起的作用。

Methods: To use ECM Gel as a tissue culture substrate, 3-dimention co-culture of the choroids plexus or the Neuro-2A cells with the ventral part of the embryonic spinal motor column (Embryonic 12 days, E12) were made in the substrate.

采用ECM Gel作为组织培养的基质底物，将脉络丛组织或者Neuro-2A细胞株和胚胎12天的腹侧脊髓运动神经柱进行三维共培养，观察脉络丛组织或者Neuro-2A细胞株对胚胎脊髓运动神经元轴突生长的影响。

The Plant tissue raise is the plant sterile culture technology, has the totipotency theory according to the vegetable cell, the use plant body exsomatize organ (for example root, stem, leaf, stem point, flower, fruit and so on), the organization (for example cambium, epidermis, cerebral cortex, marrow department cell, endosperm and so on) or the cell (for example big spore, small spore, somatic cell and so on) as well as the protoplast, in aseptic and suitable artificial culture medium and illumination, temperature and so on under artificial condition, can induce injuries the organization, not to decide the bud, the adventitious root, finally forms the complete adult plant the discipline.

植物组织培养即植物无菌培养技术，是根据植物细胞具有全能性的理论，利用植物体离体的器官(如根、茎、叶、茎尖、花、果实等)、组织（如形成层、表皮、皮层、髓部细胞、胚乳等）或细胞（如大孢子、小孢子、体细胞等）以及原生质体，在无菌和适宜的人工培养基及光照、温度等人工条件下，能诱导出愈伤组织、不定芽、不定根，最后形成完整的植株的学科。

Hairy root cultures of Taxus plants, roots of hydroponically grown Taxus plants and nodule cultures of Taxus plants are mainly introduced about the enlargements of cultural materials.

同时在毛状根培养、冠瘿瘤组织培养、水培根培养和瘤培养等培养材料的扩展方面，对国内外所作的尝试性研究和新的报道进行了综述。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。