组织培养

Result］ The new buds and petals of I.germanica was proper to be used as explant of tissue culture, with lower browning rate compared with other positions. The well situated tissue block of explant (3 mm×3 mm×3 mm) might reduce browning rate. Adding 0.1%Vc or 0.3% active carbon in medium had obvious effect on preventing tissue browning. The exogenous hormone 2 mg/L 6-BA was easy to lead to brownness turning, and 2 mg/L Kt and 2 mg/L NAA brought less effect to tissue browning. When using disinfectants of alcohol, its dipping time of 5～10 s for explant was the best, with the minimum browning rate. The culture under full illumination condition was liable to induce brownness turning, compared with other cultured conditions, the brownness turning could be restrained effectively by using imitating artificial climate condition for culture.

结果］ 鸢尾的新生芽和花瓣较适宜作组织培养的外植体，与其他部位相比褐变率较低；外植体组织块适中（3 mm×3 mm×3 mm）可降低褐变率；在培养基中加入0.1%Vc或0.3%活性炭对防止组织褐化效果明显；外源激素2 mg/L 6-BA易引发褐变，2 mg/L Kt和2 mg/L NAA对组织褐变影响不大；使用乙醇消毒剂时，对外植体浸泡时间以5～10 s为佳，褐变率最低；全光照条件培养容易诱发褐变，采用仿人工气候条件培养较其他条件培养可有效抑制褐变的发生。

Through two steps, advancing cultivation and inducing differentiation, principium founded tissue culture system of fodder beet with explant from footstalk, so established foundation for inducing polyploid fodder beet with tissue culture and enlarging reproduction.

通过预培养、诱导分化两步法，初步建立了以叶柄为外植体诱导不定芽再生的饲用甜菜组织培养体系，为进一步利用组织培养诱导饲用甜菜多倍体及多倍体扩繁奠定了基础。

Bud, leaf and leafstalk were cultured on different cultural conditions.

愈伤组织培养作了初步的探索，以不同的培养条件，利用连钱草的顶芽、叶、叶柄为外植体，研究了连钱草愈伤组织的培养。

The experiment result showed that the highest content of oleuropein was in the flower,On the basis of more than 300 times of experiments and more than 2000 test samples of data determination in this thesis, we gained the achievements in the following:1. The establishment of callus culture system and the suspension cell culture system of Syringa pubescens Turcz.

建立了小叶丁香的愈伤组织培养体系和悬浮细胞培养体系首次选择小叶丁香的叶片、叶芽和嫩茎等不同部位作为外植体，利用植物组织培养技术，在添加相应的植物生长调节剂的LS、MS等培养基上成功地诱导产生出愈伤组织，其中叶片最容易诱导产生愈伤组织，其次为芽和嫩茎。

Regeneration and reproduction by establishing tissue culture system of Betula halophila may be a promising way.

植物组织培养技术发展异常迅猛，本研究旨在建立濒危植物盐桦的组织培养再生系统，并对该盐桦组织培养再生系统进行了优化筛选，该研究为盐桦的种质资源保存和无性繁殖开辟了一条行之有效的途径。

The writer gave a detailed description of Graminaceae tissue culture history, analysized the applied tendencies and prospects about Graminaceae tissue culture basic research and, on author's work basis, pointed out the major factor influencing cereals protoplast cul...

本文拟在评述近年来禾本利植物组织培养(主要指胚胎培养、器官培养、细胞培养和原生质体培养)的理论性研究和应用性研究的进展，并重点描述和讨沦在应用上较为成熟和有发展前景的几个领域的发展现状以及利用禾本科植物的组织培养技术而进行的基因转移技术的概况。

In order to use the techniques of tissue and protoplasts culture for the plant breeding, it i prerequisite to understand the morphogenic potential of different varifies in tissue culture, and set up an efficient protoplast system capable of sustained divisi〓n and plan〓 r〓generation.

为了将组织培养和原生质体培养技术用于育种目的，必须了解不同品种的红豆草在组织培养中的再生能力和建立一个高效率的原生质体培养与再生的实验系统。

But when we examined the structure of cultivar of D. huoshanense, endomycorhiza was founded unexpectedly. And in the wild type of D. huoshanense, fungi was also founded in its stems and leaves. So the author thought that endomycorhiza is not only located in the roots of Dendiobium plants, but also exists in other organs of plants, including stems, leaves. As plant produces seeds, mycorhiza could enter into the cells of seeds. When mature seeds germinate in media, the endomycorhiza enter into the roots of cultivar.

石斛属植物与真菌共生，根为菌根，作者在研究霍山石斛组织培养型结构时发现，组织培养苗的根部也分布有真菌，而且在野生霍山石斛的茎、叶中发现有真菌，作者因而指出，真菌并不是完全分布在根部，而是遍布于整个植物体，推测植物形成种子时，真菌进入种子，所以在组织培养时，真菌随着种子的萌发，进入植物体根部。

The research on the tissue culture and cell suspension culture showed that thesuitable culture medium for inducing bud was MS supplemented with 1.5mg/L 6-BAand 0.1mg/L NAA, and for the generation-continuing multiplication was MSsupplemented with 1.5mg/L 6-BA and 0.2mg/L NAA. The rooting medium was1/2MS supplemented with 0.5mg/L IBA and the rooting rate was 45.0%. Plantletsurvival after transfer to sand was 52.5%.The induction rate of calli was66.7%～86.6% and the optimum medium was MS medium with 0.5mg/L 6-BA and2.0mg/L 2,4-D. The calli became smallest partical size, friable and had gooddispersion ability after 3 times successive transfer culture, the optimum medium wasMS medium with 0.2mg/L 6-BA and 2.0mg/L 2,4-D. Culturing these particles on sixkinds of MS liquid media with different hormone contents, the optimum medium wasselected basing on he change of the density of single-cell, the density of cellaggrefate and the mass of cell.

对蒜头果进行的组织培养与细胞悬浮培养研究结果表明：MS+6-BA1.5mg／L+NAA0.1mg／L+蔗糖3％激素组合能够较好地诱导芽的初始分化和增殖，适宜的芽苗继代增殖培养基为MS+6-BA1.5mg／L+NAA0.2mg／L+蔗糖3％；采用1／2MS+IBA0.5mg／L+蔗糖3％为生根培养基，生根率为45.0％；移栽到河沙的生根苗成活率为52.5％；愈伤组织诱导率为66.7％～86.6％，其中以MS+6-BA0.5mg／L+2，4-D2.0mg／L+蔗糖3％的培养基最佳，其诱导出的愈伤组织具有较强的增殖能力和较好的脆散结构，最佳继代培养基为MS+6-BA0.2mg／L+2，4-D2.0mg／L+蔗糖3％，且培养基中的6-BA与2，4-D浓度的比值越小，愈伤组织生长越快，结构越脆散，增殖率越高；将继代后的愈伤组织转入6种含不同激素浓度组合的MS液体培养基中进行振荡培养，在综合分析各培养基的单细胞密度，细胞团块密度，细胞生物量增长率等指标后，初步筛选出MS+6-BA0.2mg／L+2，4-D2.0mg／L培养基为较好的液体培养基。

The morphology and structure of reconstructed tissue was detected by microscope and scanning electron microscope.Results:(1) Compared with the control group, the cellular proportion of laminin group increased in 62 ~M phase, and decreased in Go~Gi phase significantly. As shown by the microscope, the cells of control group were in low density. The cells in mass connected tightly. The microfilament appeared reticular formation. The nucleus were the same in size. The cells of laminin group were in high density and put out so many lamellipodia, filopodia, which connected with the surrounding cells. The microfilament increased, elongated, and changed from reticulodromous to sarciniform, which reached to the pseudopods. The nucleus were different in size .(2) As shown by the inverted microscope and the cell growth curve, comparing with the controlgroup, cells of each test group increased evidently. The cellular proportion of each test group increased in S phase and G2 ~M phase, and decreased in Go~Gi phase significantly, but there was no considerable interations between LN and EGF;(3) As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemets membrane closely, which was similar to the natural tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately.

结果：(1)层粘连蛋白组处于G_2～M期细胞比例较对照组显著提高，Go～G_1期细胞比例显著下降，提示层粘连蛋白促进内皮细胞DNA合成，及细胞分裂增殖；光镜下，对照组细胞分布成团状，细胞密度较低，细胞间连接紧密，细胞内微丝结成网状，细胞核大小一致；与对照组相比，层粘连蛋白组细胞生长旺盛，细胞密度高，向周边伸出大量板状及丝状伪足，细胞内微丝增多、拉长、集结成束，伸入伪足中，细胞核形状大小不一致；(2)倒置显微镜观察及细胞生长曲线显示，各组细胞数目随时间增加而明显增多，各实验组较对照组增生显著，EGF和LN联合应用组各时间点细胞数目最高；实验组处于S期和G_2～M期细胞数目增加，Go～G_1期细胞数目减少；提示EGF、LN单独及联合应用均可促进细胞增殖，但尚不能认为二者有交互作用；(3)倒置显微镜下，组织培养的猫角膜内皮细胞排列成密集的单层，细胞间连接紧密；组织学观察发现，培养的猫角膜内皮细胞形成完整的内皮层，贴附于脱水基质的后弹力膜上，与正常的角膜内皮组织结构相似；扫描电镜下，组织培养的猫角膜内皮细胞间紧密镶嵌排列，可见某些细胞呈近似六边形排列，细胞大小不甚一致，胞核清晰。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。