组织发生的

The uncovered tracts and the liver wound surface were managed by some of the methods combined such as high frequency electrocoagulation, titanium clamp, suture, hemostatic gauze oppression, argon-beam coagulation, protein glue adherence, and Endo-GIA switcher. The total operative outcome, operating time, blood loss, postoperative complications and postoperative hospital stay were summarized in order to assess feasibility and safety of the clinical laparoscopic liver resection.Results: 1. Laparoscopic liver resection with clamp dissection method needs only the ordinary laparoscopic equipment. It has a low cost but causes a little more bleeding. 2. Laparoscopic liver resection operated by microwave tissue coagulation method had very little bleeding when dissecting hepatic parenchyma. The operative field is clear and the cost is low. It is a safe, convenient and effective method. 3. Ultrasound dissector is a relatively ideal appliance for laparoscopic liver resection at present because ofless bleeding during operation. But the speed is slow thus prolongs the operation time. Its separating intensity is not strong enough, and it can only be applied to patients without hepatic cirrhosis. 4. Ligsure has clamp dissection and electrocoagulation functions. It can carbonize hepatic tissue including the tracts whose diameters are less than 7mm. It has some advantage in dissecting. However, it has the shortcomings of the clamp dissector during coagulating. 5. Hand-assisted laparoscopic liver resection can make use of the flexibility and feeling of the operators left hand.

结果：1、钳夹分离切肝法不需特殊仪器设备，只要具备常规的腹腔镜器械即可实施，成本低，肝断面较易渗血；2、微波固化切肝法肝实质出血很少，视野清晰，成本低，操作简单，是一种安全、简便、有效的方法；3、超声刀是目前较为理想的切肝器械，术中出血少，但是，切割速度慢，手术时间长，而且，切割强度有限，适用于无肝硬化的肝切除；4、Ligsure具有挤压粉碎及高频电凝止血两大功能，可使包括7~以下管道的肝组织碳化、形成焦痴，具有一定的优势，但是，也存在分离钳电凝止血的缺点；5、手辅助腹腔镜肝切除可以利用术者左手的灵活性及手感，便于术中显露、分离、控制出血等，可随意协助右手及助手的主要操作，缩短了手术时间，大大提高了腹腔镜肝切除的安全性，李朝龙等的改良方法，同样达到了手辅助目的，又节省了费用；6、小切口腹腔镜辅助切肝法可以使用剖腹肝切除常规器械及剖腹肝切除技术，操作简单、可靠，适用于肝左外叶切除及右肝第V段切除；7、Endo一GIA切肝法在离断肝组织的同时闭合管道结构，多用于肝左静脉、门静脉分支、管径较大胆管的切割，缺点是不能用于较厚肝组织的切割，而且价格昂贵；8、临床巧例腹腔镜肝切除均获得成功，手术时间最短1.5h，最长sh，平均125 min，多数在100 min左右，手术出血量最少50 ml，最多500 ml，平均1 78 ml，除2例合并严重肝硬化的原发性肝癌患者术后出现少量腹水，1例术后发生胆漏外，其余无并发症，发生胆漏的1例患者术后住院40d，多数在术后一周左右出院，术后住院时间5一40d，平均gd。

Content of flavone is the hightest in browning callus. It is supposed, that suppose which the last pace of synthesize flavone is in brovwing callus. SDS-PAGE cataphoresis electrophoresis show protein has big change from differentiating callus to browning callus. The change may has relation with change of enzymire activiation of control flavone synthesize.

黄酮含量的变化以褐化愈伤组织中含量最高，因此我们推测：黄酮合成的最后几步，可能发生在褐化愈伤组织中，从可溶性蛋白质的 SDS-PAGE 平板电泳结果表明，分化愈伤组织与褐化愈伤组织相比蛋白质谱带发生了明显的变化，这可能与控制黄酮合成的有关酶的活性变化有关。

As the body response for the trauma, the adipose tissues as blood serum behavior in the wounded tissue were suffered mobilization. At the same time, the ectogenesis fat tissue entranced blood circulation. As the result of hemodynamics alteration, there were blood platelet and erythrocyte and leucocytes and lipid granules collecting on the surface of these lipid droplets. The volumes of these lipid droplets were enlargement. These lipid droplets were changed to fat embolus by the promotion of coagulation and fibrin deposition intra-blood vessel.

现代医学理论认为，外伤后组织存留的血清状态的脂肪组织受到动员，同时，损伤部位的血管外源性脂肪进入血循环，而创伤引起的机体反应使血液动力学发生改变，血小板、红细胞、白细胞及血脂乳化不稳定所析出的脂质颗粒等，均可聚集于以上脂滴的表面，使之体积增大，加之组织凝血活化酶的释放，促使血管内凝血，纤维蛋白沉积的发生，从而形成脂肪栓子，导致脂肪栓塞。

This study clearly showed that mosaic variation was caused by chlorophyll deficiency mutation of shoot tip histogen layer-Ⅱ;tissue and organs originated from the layer-Ⅱ couldn't synthetize chlorophyll,thus induced albino; the albino variation appeared yellow green mosaic because layer-Ⅱ was displaced by layer-Ⅲ partly.

研究表明，其变异原因是由于茎尖分生组织的L -Ⅱ组织原层发生失绿突变，致使其衍生的组织和器官不能合成叶绿素，从而形成白化现象；又由于L -Ⅱ与L -Ⅲ组织原层层间发生部分代换，致使白化突变呈黄绿镶嵌的花斑。

For the BM-rice,the sclerenchyma of leaves was shrunken,vascular bundle of leaves was reduced,the strumae of outer epidermis of stem was sparse and the link of phloem of root was loosed and dispersed.

2通过扫描电镜观察，脆性突变水稻的组织结构发生了较大变化：叶片薄壁组织扩张，厚壁组织收缩，维管束数目减少；茎杆外表皮硅质瘤状结构分布稀疏；根中柱韧皮部组织排列稀疏，连接松散。

The results were showed that embryogenic calli were induced from young leaves, which were cultured on MS medium supplemented with 2,4-D 2mg/L and KT 0.2mg/L. For the proliferation of embryogenic calli, the suitable culture medium was MS+BA 8mg/L +NAA 1mg/L. The development and maturation of somatic embryo could be much improved by using the medium of MS+ZT 2mg/L or BA 5mg/L +IAA 0.2mg/L. For the induction of secondary somatic embryo from integral somatic embryo, the suitable culture medium was MS+KT 0.1mg/L+NAA 0.01 mg/L or MS+ZT 0.1mg/L+NAA 0.01 mg/L, the proliferation frequency is 214%, 256% respectively. The cotyledonary generated from somatic embryos of Aesculus hippocastanum L.

本文就欧洲七叶树的组织培养和体细胞胚发生以及植株再生进行了系统研究，主要结果如下：以植物细胞具有全能性的理论为依据，以欧洲七叶树幼嫩叶片为外植体，进行体细胞胚胎发生研究，研究结果表明，诱导愈伤组织的适宜培养基是MS+2，4-D 2mg／L+KT0.2mg／L，MS+BA 8mg／L+NAA 1mg／L有利于胚性愈伤组织的诱导和增殖，添加ZT 2mg／L或BA 5mg／L和IAA 0.2mg／L的MS培养基有利于体细胞胚发育和成熟，体细胞胚可直接诱导次生胚发生，MS+KT 0.1mg／L+NAA 0.01 mg／L或MS+ZT 0.1mg／L+NAA 0.01 mg／L培养基诱导效果最好，增殖频率分别为214％、256％。

RESULTS: In the SH-rhBMP-2 group, sparse chondroid tissue could be found at the 2 weeks of operation, which arranged orderly along the tibial tunnel from 4 to 8 weeks, tendon adhered to adjacent tissue stably, and bony tissue was appeared at the 12 weeks.

结果：透明质酸钠-重组人骨形态发生蛋白2亚组在术后2周隧道内见稀疏软骨样组织，4~8周时隧道内见沿骨隧道排列的软骨样组织，双股肌腱之间形成稳定组织连接。l2周时出现部分骨样组织包裹肌腱。

With many years of research, two inbreeds 18-599 and 18-599 are sieved to be excellent on both characters of induction efficiency and number of regenerating. They also act much better comparing with those superior acceptors as B73, A188 and zong 31, which are known in foreign and home country, and their induction efficiency don't act different essentially, however their number of regenerating is much lower.

6经过多年的重复研究后筛选到的玉米幼胚培养胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数等2个幼胚培养能力性状均表现十分优良的自交系18-599和18-599，与国内外公认的玉米转基因优良受体，如B73、A188和综31、中自03等相比较，它们不仅在幼胚培养胚性愈伤组织诱导率表现上没有实质性的差别，而且在胚性愈伤组织绿苗发生数的表现上要比后者优良得多。

Based on the enterprise of understanding the concept of crisis management awareness, and post-clearance business and the complex relationship between media and corporate crisis management in response to the media about the need for rapid response and respect for the media a number of basic principles, such as one by one analysis, and a general joint professional public relations companies and professionals, such as to clarify the position of a number of organizations to deal with the media approach Skills, itemizing.

当危机发生时，企业必须对此引起高度重视，努力去处理好与之相关的各个公众组织的关系。在处理危机事件时，毫无疑问，媒介是企业最需要关注，与之处理好关系的一个组织，因为它起着企业与其他组织之间沟通的一个很重要的桥梁的作用。一旦企业发生危机事件，媒介必然介入。在发生危机时努力处理好与媒介的关系，对企业来说是非常重要的。

All these results indicate that the DNA variation process during the occurring process of adenocarcinoma may be: the stable DNA phosphate backbone in normal gastric mucosa change into unstable DNA phosphate backbone in intestinal metaplasia issue under affecting of pathogenic factors, and finally, DNA phosphate backbone is broken and re-forms a double stabilized phosphate backbones, which causes the occurring of adenocarcinoma.

这些结果提示胃癌的发生过程中DNA结构变化过程可能是：胃粘膜正常组织具有稳定的DNA磷酸骨架，在致病因素作用下发展为具有不稳定磷酸骨架DNA的肠化组织，最后组织DNA磷酸骨架断裂并重新形成稳定的DNA磷酸骨架，致胃癌发生。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。