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Result] The new buds and petals of I.germanica was proper to be used as explant of tissue culture, with lower browning rate compared with other positions. The well situated tissue block of explant (3 mm×3 mm×3 mm) might reduce browning rate. Adding 0.1%Vc or 0.3% active carbon in medium had obvious effect on preventing tissue browning. The exogenous hormone 2 mg/L 6-BA was easy to lead to brownness turning, and 2 mg/L Kt and 2 mg/L NAA brought less effect to tissue browning. When using disinfectants of alcohol, its dipping time of 5~10 s for explant was the best, with the minimum browning rate. The culture under full illumination condition was liable to induce brownness turning, compared with other cultured conditions, the brownness turning could be restrained effectively by using imitating artificial climate condition for culture.

结果] 鸢尾的新生芽和花瓣较适宜作组织培养的外植体,与其他部位相比褐变率较低;外植体组织块适中(3 mm×3 mm×3 mm)可降低褐变率;在培养基中加入0.1%Vc或0.3%活性炭对防止组织褐化效果明显;外源激素2 mg/L 6-BA易引发褐变,2 mg/L Kt和2 mg/L NAA对组织褐变影响不大;使用乙醇消毒剂时,对外植体浸泡时间以5~10 s为佳,褐变率最低;全光照条件培养容易诱发褐变,采用仿人工气候条件培养较其他条件培养可有效抑制褐变的发生。

It was observed that the influence of bud and its position on the healing process of grafting unions using hardwood sectioning method, and found that:(1) bud of a scion and its position to the graft unions have no influence on callusing formation of the isolation layers, and the early differentiation of cambia;(2) a scion bud closer to the grafting union would promote the formation of more compacted callus cells and the earlier formation of callus bridges;(3) conduit differentiation in grafting unions of lower-bud scions was later than that of other treatments.

利用滑动切片法对芽子有无及芽位对嫁接愈合过程的影响进行了观察,发现:(1)接芽有无及芽位对隔离层及愈伤组织形成和形成层的早期分化没有影响;(2)芽或去芽的芽位距接口近,可以促进愈伤组织的紧密连接,即愈伤组织桥的形成;(3)低位芽枝嫁接体中导管分化较晚,而其它处理导管的分化几乎是同时发生的,而且导管首先出现于愈伤组织薄壁细胞中。

The results showed that LRK could significantly inhibit the mammary hyperplasia caused by estrin, reduce the content of serum 〓, raise P level, promote the production of DA in hypothalamus and mammary tissue, decrease the release of 5-HT, inhibit the secretion of serum PRL, reduce the sensitivity of mammary tissue to estrin and progestogen, prevent the expression of mammary tissue on PCNA, the effect was equal to triphenylamine oxide, obviously superior to the control group of Ru Zeng Ning.

通过实验研究,建立了具有肾虚肝郁证侯群的乳腺增生病理模型,利用免疫组化技术、图像分析系统、荧光分光光度法、放射免疫法等现代医学研究方法,从病理形态学、医用体视学、神经内分泌学、阴道细胞学等方面,观察了利乳康口服液对模型动物整体状态、乳腺组织病理形态、血清激素、神经递质以及卵巢功能的影响,结果表明利乳康口服液能明显抑制雌激素引起的乳腺组织增生,降低血清E〓含量,升高P水平,促进下丘脑及乳腺组织中DA的产生,减少5-HT释放,抑制血清PRL分泌,降低乳腺组织对雌、孕激素的敏感性,阻止乳腺组织PCNA的表达,其效应与三苯氧胺相当,明显优于乳增宁对照组;另外,利乳康口服液还能缓解模型动物肾虚肝郁证侯群,增加其体重,调节其整体状况,改善模型动物不规则动情周期,减轻其子宫及卵巢的病变,抑制子宫内膜的过度增长,调节卵巢功能,揭示了中药利乳康口服液对神经内分泌和卵巢功能的整体调控作用,以及对本病发生、发展的预防作用

Abnormal Fhit gene expression may be the early molecular event, it may play a role in oncogenesis of laryngocarcinoma and transformation to cancer, however, is not further deteriorative following disease advancing.

Fhit基因改变可能是喉癌发生的早期分子事件,作用于肿瘤发生过程中,在喉正常组织向癌组织演变过程中发挥作用,并不随着肿瘤的深化,淋巴结转移而进一步增强。

CD8~+ cell is the main T lymphocyte subset in spleen, and B lymphocyte mainly is IgG~+ cell, moreover the amount of these B lymphocytes could exceed CD3~+ T lymphocyte subset after 7 days. CD8~+ cell is the main T lymphocyte subset in tonsil of appendix, and B lymphocyte is IgM~+ cell, and the amount could exceed CD3~+ T lymphocytes after 35 days. After 21 days, B lymphocytes in esophago tonsil are the main IgA~+ cells and the amount exceeds CD3~+ lymphocytes. The amount of CD4~+ lymphocytes is more than CD8+ lymphocytes.4. CD3~+、CD4~+ and CD8~+ T lymphocytes in spleen mainly distribute in periarterial lymphoid sheath. However IgM~+、IgG~+ and IgA~+ cells mainly distribute in ellipsoid periarterial lymphoid sheath and germinal center. T lymphocytes in appendix tonsil mainly distribute in middle and inferior part of mucous and the B lymphocytes mainly in middle and mucous between 4~7 days. Whereafter T, B lymphocytes equably distribute in mucous. CD4~+ cells arrange tightly and mainly occupy the central part in aggregates of T lymphocytes in esophago tonsil and CD8~+ lymphocytes mainly distribute in periphery. Meanwhile B lymphocytes encircle the periphery of aggregates of T lymphocytes. The aggregates of B lymphocytes is mainly the germinal center with lots of IgM~+、IgG~+ and IgA~+ cells. Meanwihle T lymphocytes encircle the periphery of aggregates of B lymphocytes.5. There is an intimate relationship between the development of tissue structure of peripheral immune organs and lymphcytopoiesis. The maturation of tissue structure is stimulated by the immigration of lymphocytes and the mature tissue structure provides place where lymphocytes grow mature and functionate.

脾脏在21日龄时达到稳定,食管扁桃体和盲肠扁桃体均在35日龄时达到稳定;脾脏中T淋巴细胞亚群以CD8~+细胞为主,B淋巴细胞则以IgG~+细胞为主,并在7日龄后数量超过CD3~+T淋巴细胞;盲肠扁桃体中T淋巴细胞亚群以CD8~+细胞为主,B淋巴细胞以IgM~+细胞为主,并在35日龄后数量超过CD3~+T淋巴细胞;21日龄后,食管扁桃体中B淋巴细胞以IgA~+细胞为主,数量超过CD3~+细胞,CD4~+细胞的数量多于CD8~+细胞。4、在T、B淋巴细胞组织定位方面,脾脏中CD3~+、CD4~+和CD8~+T淋巴细胞主要分布在动脉周围淋巴鞘中,而IgM~+、IgG~+和IgA~+细胞主要分布在椭球周围淋巴鞘和生发中心中;4~7日龄时,盲肠扁桃体中T淋巴细胞主要分布在粘膜固有层的中下部区域,而B淋巴细胞则主要分布在中上部区域,随后各日龄T、B淋巴细胞均匀地分布在粘膜固有层中;在食管扁桃体的T淋巴细胞聚集物中,CD4~+细胞紧密排列,主要占据中央部位,CD8~+细胞主要散布在外周,同时B淋巴细胞又环绕在整个T淋巴细胞聚集物的外周;B淋巴细胞聚集物主要为生发中心,其中存在大量IgM~+、IgG~+和IgA~+细胞,同时T淋巴细胞又环绕在整个B淋巴细胞聚集物的外周。5、外周免疫器官的组织结构发育和淋巴细胞发生之间存在密切的关系,淋巴细胞迁入淋巴器官刺激组织结构的发育成熟,同时成熟的组织结构又为淋巴细胞发育成熟并行使功能活动提供场所。

In conclusion, Kikuchi-Fujimoto disease can masquerade as a single, rapidlygrowing, parotid tumor in a middle-aged man. Because it is histiocytic necrotizing lymphadenitis adjoining the parotid gland, Kikuchi-Fujimoto disease should be considered a cause of parotid tumor.

经此案例可知,菊池-藤本氏病可以单一、快速增大,发生在中年男性且以疑似腮腺肿瘤为表徵,实际上是邻近腮腺的组织发生了组织球性坏死性淋巴结炎,故临床上耳下附近的疑似腮腺肿瘤病灶,应将本病列入鉴别诊断。

The result shows that modification can make the β-Si phase thin when the joining amount is 0.3wt%, making the best modification result. Alloying elements Mn and Ni are always used to neutralize iron"s harm in aluminum alloys. They can form many kinds of complicated metallically compounds with Fe and Al, and eliminate needle-like iron-rich phases to reduce iron"s harm in Al-Fe alloy. Copper makes the alloy microstructure thick and electromagnetic stirring obviously makes the alloy microstructure thin, round the second phase, when the stirring voltage is 200V, making the best result; Reheat makes liquid phases emerge in the alloy, when the warming temperature is 650?

研究结果表明:红磷可以明显细化合金中的初晶硅,当加入量为0.3wt%时,取得最佳变质效果;锰、镍合金中加入后,通过形成多种复杂金属间化合物,细化针片状富铁相,减小铁对铝硅合金性能的危害;铜使合金微观组织发生粗化;电磁搅拌明显细化合金组织,使第二相圆整化,搅拌电压为200V时,取得最佳细化效果;二次加热使合金组织中产生部分液相,在650?

It was studied that the process of the somaticembryogenesis and analyzed that the problem existing in technique of in vitro morphogeny in Hevea to ground for that to probe into the method to improve the technology of tissue culture in Hevea with the techniques of transgene and to improve the ability of somatic embryogenesis and plant percentage of embryoid in Hevea.

本试验以巴西橡胶树,海垦2的花药为外植体,诱导体胚的发生,对体细胞胚发生方式及发育过程做了一系列的组织学观察,分析了橡胶树体细胞胚发生的各个过程以及畸形胚的形成原因,初步结果如下: 1 橡胶花药体细胞胚主要以间接方式发生。

Methods Immunohistochemistry was used to detect Tiam 1 expression in 89 cases of primary tumor and metastatic lymph node of thyroid carcinoma.

结果淋巴结转移癌组织中Tiam1的表达显著高于甲状腺癌原发灶中的表达(P.05);伴发转移的甲状腺癌组织比未发生转移的甲状腺癌组织的Tiam1表达明显增强(P.05);甲状腺髓样癌、未分化癌组织中Tiam1的表达显著高于乳头状癌、滤泡癌中的表达(P.05);高临床分期甲状腺癌组织中的Tiam1表达明显高于低临床分期中的表达(P.05)。

The results show that B2 phase presents broad fibred shape in the forged rod, and major textures are close to {112}〈111〉and {110}〈111〉; Fibred microstructure in hot-extruded tube becomes fine and fibres have broken, at the same time,{111} orientation of many grains is close to axis direction of tube; Microstructure in cold-rolled tube consists of B2,β-Nb and4Ni2O phases, fibres has heavily broken, which restrains phase transformation during cooling and heating.

结果表明:锻棒中的B2相呈较宽的纤维状,主要的织构组分接近{112}〈111〉和{110}〈111〉;热挤压管纤维组织变细且发生碎化,多数晶粒的{111}取向接近管材的轴向;冷轧管相组成为B2、β-Nb和4Ni2O,纤维组织发生严重碎化,抑制了马氏体相变的进行,{111}〈110〉和{112}〈110〉成为主要的织构组分。

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And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

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