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The microstructure in the heat--affected zone consists of coarse bar-shape recovery grain, and that of the thermo-mechanically affected zone exhibits bent band-like character, but the deformation degree at the advancing side is bigger than that at the retreating side. Microstructures in TMAZ are also recovered, and the amount of recovery grain at retreating side is more than that at advancing side.

热影响区组织发生回复和粗化反应,形成粗大的棒状回复晶粒;热机影响区组织发生弯曲变形,但整体上仍保留带状组织形貌,前进侧热机影响区组织变形程度高于后退侧,该区同时还发生回复反应,且后退侧热机影响区内的回复晶粒数量多于前进侧。

B Of the six basic media MS, MS1/2 (half-strength of MS salts and vitamins), WPM, DKW, B5 and SH, MS1/2 was the most proper one to induce somatic embryos. Somatic embryos generally regenerated directly from excised zygotic cotyledons. PGRs combination affected somatic embryogenesis significantly. Medium with NAA 1mg/L, TDZ 0.05mg/L, IBA 2—10mg/L combined with BA 10mg/L, or IBA 10mg/L integrated with BA 0-2mg/L gave the highest induction rate. Excised zygotic hypocotyls had the strongest potential to produce callus. Callus induction was also affected significantly by media and PGRs. The proper callus induction condition was MS1/2 medium containing NAA 1mg/L, IBA 10mg/L, BA 2-5mg/L and TDZ 0.05mg/L. Harvest period affect somatic embryogenesis significantly. Zygotic embryo explants collected from the end of July to the middle of August had strong potential to generate somatic embryos, when endosperm finished solidification, different parts of the embryos were completely formed, the size of embryos occupied about 2/3 of the embryo sac. Provided with optimized conditions, direct somatic embryogenesis rate can attain to 33. 68%, and callus induction rate of hypocotyls was up to 90.7%. Cytological observation on megasporogenesis and zygotic embryogenesis of Manchurian ash showed that the ovary was twicarpellum, twilocular with two ovules each loculus. The ovule was tenuinucellar and anatropous, with one megasporcocyte. The development of embryo sac is of the Polygoum type.

体细胞胚胎发生研究的结果表明:(1)成熟过程中的合子胚是诱导水曲柳体细胞胚胎发生的最佳外植体材料;(2)在所试验到的MS、MS1/2(将MS的所有成分均减半)、WPM、DKW、B〓、SH等六种基本培养基中,MS1/2是最适合诱导水曲柳体细胞胚胎发生的基本培养基;(3)水曲柳的体细胞胚胎发生以直接发生为主,体细胞胚主要来自于从合子胚分离的完整子叶;(4)培养基中的激素组合对水曲柳的体细胞胚胎发生有显著影响,诱导直接体细胞胚发生较好的激素组合有NAA 1mg/L+IBA 2,5,10mg/L+BA 10mg/L+TDZ 0.05mg/L和NAA 1mg/L+IBA 10mg/L+BA 0,2mg/L+TDZ 0.05mg/L;(5)合子胚分离的下胚轴具有最强的愈伤组织诱导潜力,少数愈伤组织可以分化出体细胞胚;(6)愈伤组织的诱导也受培养基和激素配比的显著影响,最适宜诱导的培养条件为MS1/2+NAA 1mg/L+IBA 10mg/L+BA 2,5mg/L+TDZ0.05mg/L;(7)采种时间对体细胞胚胎发生有显著影响。7月末到8月中旬的合子胚具有较强的体细胞胚发生潜力,此时种子尚未成熟,胚乳已呈固态,种胚的各个部分已分化完全,种胚体积占胚腔的大约2/3;(8)在各自综合的最适条件下,完整子叶的体细胞胚诱导率可达33.68%,下胚轴的愈伤组织诱导率可达90.7%。

Objective To explore livin, MTA1 and the caspase3 protein expression, their correlation and clinical pathology in colon cancer Methods The expressions of livin, MTA1 and caspase3 protein in 88 cases of colon cancer were detected by immunohistochemistry stainingResults Livin, caspase3 protein and MTA1 positive expression rates in colon cancer tissues were more than those in tissues beside the cancer Furthermore there were obvious relevance between livin protein, MTA1 positive expression rate and degree of histodifferentiation and lymph node metabasis and between caspase3 protein positive expression rate and degree of histodifferentiation of colon cancer Caspase3 protein expression had prominent inverse correlation with the livin expressionConclusions ①Over expression of livin having inhibtion on colon cancer is one of important factors of colon carcinogenesis ②Caspase3 protein expression in colon cancer tissues is inhibited to less and has prominent inverse correlation with livin expression Accordingly, suppressing caspase3 protein activity is one of channels, by which livin promotes colon carcinogenesis ③MTA1 plays the important role in histodifferentiation degree and lymph node metastasis of colon cancer

采用免疫组织化学染色方法检测88例结肠癌组织中Livin,Caspase3 及MTA1的表达情况。结果(1)结肠癌组织中Livin的阳性表达率显著高于癌旁组织,且Livin的表达与结肠癌的组织分化程度及淋巴结转移程度显著相关。(2)结肠癌组织中Caspase3蛋白的阳性表达率显著高于癌旁组织,且Caspase3蛋白的表达与结肠癌的组织分化程度显著相关。(3)结肠癌组织中MTA1的阳性表达率显著高于癌旁组织,且MTA1的表达与结肠癌的组织分化程度及淋巴结转移程度显著相关。(4)Caspase3蛋白的表达又与Livin的表达呈显著负相关。结论(1)Livin在结肠癌组织中过表达,它可能是促进结肠癌发生的重要因素之一。(2)Caspase3蛋白在结肠癌组织中表达被抑制而降低,且与Livin的表达呈负相关。因此,抑制Caspase3蛋白的活性是Livin促进结肠癌发生的途径之一。(3)MTA1对结肠癌的组织分化及淋巴转移发挥重要作用。

Objective To investigate the relationship between neuroendocrine differentiation of ovarian mucinous tumors and its genesis.

目的 探讨卵巢粘液性肿瘤的神经内分泌分化与肿瘤分化程度、组织发生的关系。

The results showed that the number of chromosome, which was from the cultured seedlings of meristem tip and the rank seedlings of meristem tip, was not altered, both the number were 2n=16,the hereditary stability of callus and regeneration plant was poor; and the variation percentage of chromosome in callus was 43.4%, among which haploid accounted for 6.7%, triploid accounted for 2.5%,tetraploid accounted for 10%,pentaploid accounted for 4.2%,hexaploid accounted for 3.3%,septuploid accounted for 4.2%,octoploid accounted for 3.3%,dysploid accounted for 9.2%;the chromosome variation percentage of differentiation seedlings derived from callus was11.7%,among which haploid was 6.7%,triploid accounted for 1.7%,tetraploid was 3.3%.

结果表明,茎尖分生组织培养的幼苗及丛生苗遗传稳定,其染色体未发生倍性变异,均为2n=16;愈伤组织及其再生苗遗传稳定性较差,愈伤组织染色体数变异率为43.4%,其中单倍体占6.7%、三倍体占2.5%、四倍体占10%、五倍体占4.2%、六倍体占3.3%、七倍体占4.2%、八倍体占3.3%、非整倍体占9.2%;愈伤组织分化苗染色体变异率为11.7%,其中单倍体占6.7%,三倍体占1.7%,四倍体占3.3%。

Expressions of PPAR-γ mRNA and its protein in lung were decreased significantly after phosgene exposure.

光气染毒后大鼠肺组织PPAR-γ基因及蛋白表达显著降低,这可能与光气诱导肺组织发生的炎症反应有关。

Objective To analyse the oncocyte appeared in the fine needle aspiration cytology samples,discuss the morphologic and histogenetic features.

目的 对针吸细胞学标本中出现的嗜酸性细胞进行分析,探讨其形态学特征和组织发生的本质特性。

The triphasic nature of Wilms Tumor is obious here.

Wilms'瘤也称作肾母细胞瘤,是由肾胚芽组织发生的恶性混合型肿瘤,最常见于2~4岁小儿,多单侧发病。

The key problem for limb , skin or other composite tissues transplantation is to control posttransplantation rejection , which encumbers long term functional survival of transplanted organ and tissues .

作为皮肤复合组织移植的受者,术后出现排斥反应的几率很高,排斥反应发生的程度和频率将直接影响移植组织能否长期存活及功能能否及时恢复,而免疫抑制剂的个体化用药是否合理又直接影响术后免疫排斥反应发生的频率和程度,因此为受者&量体裁衣&制定免疫抑制剂术后个体化给药方案,是保证移植组织或器官有功能性的长期存活的关键因素之一。

The authority depends on the ownership for itsexistence, which means the stockholder of the company owns the authority and coulddetermine the alloc...

企业组织里的权威或者控制权是可以转让的,控制权分配发生于组织内的委托人和代理人之间,更具体一点的讲是发生在组织内的领导和下属之间。一个不断。。。

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