组织原

Disk height index of intervertebral disks on X-ray films, areas and average T2 relaxation time of high intensity zone of disks on T2-weight MRI and histological score on pathological sections （HE staining, Masson staining and safranin O-fast green staining） were measured at 2 months, 4 months and 6 months after operation.

术后2、4、6个月时对正常及模型椎间盘观察X线片上的椎间盘高度指数、MRIT2加权相上的椎间盘高信号区的面积和平均T2弛豫时间、椎间盘纤维环损伤区的形态学变化、组织病理切片（HE染色、Masson三色胶原染色和番红O-固绿染色）椎间盘组织形态学变化、生物化学变化和组织学评分等指标。

RESULTS: Microscopically, the lesion consisted of abundant collagen fibrils, proliferated histiocytes and numerous infiltrated plasmocytes, lymphocytes and eosinophilic granulocytes. Fusiform or polygonal histiocytes had abundant, granular, eosinophilic cytoplasm or clear cytoplasm containing radially oriented strands or resembled xanthoma cells. Emperipolesis of numerous small lymphocytes was seen in the cytoplasm of some large histiocytes.

结果： 镜下可见丰富的胶原纤维和组织细胞增生以及大量浆细胞、淋巴细胞和嗜酸粒细胞浸润，组织细胞呈梭形、多边形，胞质嗜酸性颗粒状、放射条纹状或泡沫状，一些大组织细胞质内有多量小淋巴细胞。

RESULTS摘要: Microscopically, the lesion consisted of abundant collagen fibrils, proliferated histiocytes and numerous infiltrated plasmocytes, lymphocytes and eosinophilic granulocytes. Fusiform or polygonal histiocytes had abundant, granular, eosinophilic cytoplasm or clear cytoplasm containing radially oriented strands or resembled xanthoma cells. Emperipolesis of numerous small lymphocytes was seen in the cytoplasm of some large histiocytes.

结果摘要：镜下可见丰富的胶原纤维和组织细胞增生以及大量浆细胞、淋巴细胞和嗜酸粒细胞浸润，组织细胞呈梭形、多边形，胞质嗜酸性颗粒状、放射条纹状或泡沫状，一些大组织细胞质内有多量小淋巴细胞。

Adjacent explants were connected to each other by the migrating cells with the occurrence of autorhythmic beating of the explants.

在三维胶原中，心脏组织块生长良好，迁出的细胞将相邻组织块连接起来，组织块有自发的搏动。

Cardiac explants grew well in the collagen gel. Adjacent explants were connected to each other by the migrating cells with the occurrence of autorhythmic beating of the explants.

在三维胶原中，心脏组织块生长良好，迁出的细胞将相邻组织块连接起来，组织块有自发的搏动。

Methods S-P immunohistochemical staining was used to detect the expression of VEGF-C and its reˉceptor Flt-4in10neighboring noncancerous tissue and60NSCLC cases of non-small cell lung cancer and32lymph nodes cases with lymph nodes metastasis of non-small cell lung cancer.

采用免疫组化S-P法检测60例NSCLC原发灶组织、10例癌旁组织、32例伴有肺癌转移的淋巴结组织中VEGF-C、Flt-4的表达，并将结果与肺癌的临床病理特征进行分析。

Methods S-P immunohistochemical staining was used to detec t the expression of VEGF-C and its reˉceptor Flt-4in10neighboring noncancerous tissue and60NSCLC cases of non-small cell lung cancer and32lymph nodes cases with lymph nodes metastasis of non-small cell lung cancer.

采用免疫组化S-P法检测60例NSCLC原发灶组织、10例癌旁组织、32例伴有肺癌转移的淋巴结组织中VEGF-C、Flt-4的表达，并将结果与肺癌的临床病理特征进行分析。

PartⅡ: Analysis of the paraffin embeded tissue slides [obtained from trail and control group at pre-treatment (d0) and post-operation (d15±1 days) ] and frozen section slides (from 4 typicalcases: 1 of IAC group, the other sBRM group at d5, d7 and d10), by H-E stain, monoclonal antibodies (CD3/CD4/CD8/CD68/CD57) marking, SP-DAP or APAAP-AEC stain, and TUNEL in situ apoptotic cell DNA fragments fluorescin labeling.

二。组织病理学研究：对上述两组病人治疗前后原发灶肿瘤组织蜡块分别作H-E和CD3、CD68表型免疫组化染色；并对其中四例典型病人的疗中间断连续活检新鲜组织作H-E染色，CD3、CD4、CD8和CD57表型免疫组化染色和凋亡细胞DNA断片TUNEL法荧光标记检测。

Results Of the 52 specimens, 38 were located in the ciliary body (73.1%), and 14 lesions involved the iris (26.9%). According to the results of histogenetic classification, the tumors of ciliary sensory epithelium were ranked the first place (20 cases, 38.5%) while malignant melanoma, the second one (14 cases, 26.9%) followed by melanocytoma (9 cases, 17.3%) and granulomatous lesions (5 cases, 9.6%).

结果 52例组织标本按解剖部位分类：位于睫状体病变38例（73.1%），原发于睫状体累及虹膜组织14例（26.9%）；按组织来源分类：占第1位者为睫状体神经上皮来源的肿瘤20例（38.5%），第2位者为恶性黑色素瘤14例（26.9%），第3和第4位者分别为黑色素细胞瘤9例（17.3%）和肉芽肿性炎性病变5例（9.6%）。

Methods: The experimental type Ⅱ diabetic rats were induced by injection of strepiozotocin (STZ, 30mg/kg ) and fed with high fat and high glucose fond. The rats were divided into three groups: a normal conrmal control group, a diabetes group and a treated group. The treated group were hypodermically injected insulin 3U/d at interfemus for 4 months, white DC and NC group were vehicle groups that were treated with the same close saline, By using SABC method, thee express ion of collagen type Ⅰ and Ⅲ in SAN was detected.

高糖高脂饮食加小剂量链脲佐菌素腹腔注射(STZ，30mg/kg)制备Ⅱ型糖尿病大鼠模型，并随机分为正常对照组、糖尿病组和胰岛素治疗组（每只3U/d股内侧皮下注射），对照组和糖尿病组注射等剂量的生理盐水每天1次，持续4个月；窦房结组织行SABC法免疫组织化学显色，检测窦房结组织中的Ⅰ型和Ⅲ胶原的表达及变化。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。