组织原

Objective To investigate the proliferation of porcine meniscus fibrochondrocytes and the expression of collagen under the condition of monolayer culture to fabricate autologous tissue-engineered menisci.

目的 研究体外单层培养条件下猪半月板纤维软骨细胞增殖和胶原的表达，为构建自体组织工程化半月板奠定基础。

Methods: The hEGcs from the gonadal ridges and dorsal mesenteries of human embryos aged 5-10 weeks were cultured with or without cytokine in culture medium to observe their growth and the effects of different cell culture systems were compared.

取5~10周人胚胎生殖腺嵴和背侧肠系膜，用添加和不添加细胞因子的两种培养体系对比进行组织块原代及传代培养，选取生长情况良好的传至第4代的人EG细胞，移植到急性心肌梗死大鼠的梗死心肌周围，分别于移植后1天、1、2、4周处死大鼠，以鼠抗人细胞核抗体MAB1281作为示踪剂，通过免疫组化方法检测移植细胞的分布。

Methods Primary human embryonic germ cells were obtained by tissue culturing of embryonic genital ridges and mesenteries from the embryos aged 8 weeks, and then were passaged on the 8th and 16th day using pick-up and digestive passage method. Three kinds of digestive juice were used.

取8周人胚胎的生殖腺嵴和肠背系膜，进行组织块体外培养人胚胎生殖细胞，选择原代培养第8天和第16天的细胞，采用挑取传代法和消化传代法，其中的消化传代法采用三种不同的消化液进行传代培养，记录各代培养的细胞集落数。

Methods: the hegcs from the gonadal ridges and dorsal mesenteries of human embryos aged 5-10 weeks were cultured with or without cytokine in culture medium to observe their growth and the effects of different cell culture systems were compared. the hegcs' from passage 4 were transplanted directly to infarcted myocardium.

取5~10周人胚胎生殖腺嵴和背侧肠系膜，用添加和不添加细胞因子的两种培养体系对比进行组织块原代及传代培养，选取生长情况良好的传至第4代的人eg细胞，移植到急性心肌梗死大鼠的梗死心肌周围，分别于移植后1天、1、2、4周处死大鼠，以鼠抗人细胞核抗体mab1281作为示踪剂[1]，通过免疫组化方法检测移植细胞的分布。

A mouse was chosen and moulted, and then irradiated by the IPL system in vivo under certain wavelengths with different energy densities. The morphologic changes of mouse skin were observed before and after (1 day, 3 days, 7dys) irradiated respectively. The results were compared and analyzed. The function of dermal collagen during the renovating of the tissue was studied and the key factors were presented.

以小白鼠作为研究对象，先去毛，用IPL在一定波长不同的能量密度照射活体小白鼠皮肤，分别在照射前、照射后，以及之后的1天，3天，7天应用激光共聚焦显微镜观察皮肤内部的结构，并对其不同的能量密度与照射前相比进行分析，讨论了真皮胶原蛋白在组织的修复过程的作用及其中的关键因素。

A mouse was chosen and moulted, and then irradiated by the IPL system in vivo under certain wavelengths with different energy densities. The morphologic changes of mouse skin were observed before and after (1d, 3d, 7d) irradiated respectively. The results were compared and analyzed. The function of dermal collagen during the renovating of the tissue was studied and the key factors were presented.

以小白鼠作为研究对象，先去毛，用IPL在一定波长不同的能量密度照射活体小白鼠皮肤，分别在照射前、照射后，以及之后的1 d，3 d，7 d应用激光共聚焦显微镜观察皮肤内部的结构，并对其不同的能量密度与照射前相比进行分析，讨论了真皮胶原蛋白在组织的修复过程的作用及其中的关键因素。

Methods Rats were infected with MP for 9 times during a period of 24 weeks by ultrasonic nebulizing inhalation to establish animal models of chronic pulmonary MP infection. In situ hybridization was performed with bFGF cDNA probe and results were quantitatively analysed to measure the changes of bFGF mRNA expression in lung tissues.

将8只大鼠于24周内反复9次经超声雾化吸入肺炎支原体以复制其慢性肺部感染模型，另以4只为对照，随后用bFGF cDNA核酸探针行核酸分子原位杂交及定量图像分析，观察肺组织bFGF mRNA表达的变化。

The tissue responses to MTA were compared with that to amalgam. In 16 premolar teeth of dogs (32 roots), the root canals were cleaned, shaped and obturated under ideal condition.

第一部分：MTA倒充填后根尖周组织的愈合反应和非胶原蛋白的表达4只健康杂种犬，双侧下颌第三、第四双尖牙作为实验牙。

The second passage was obtained after the cell overgrew the bottom of the culture flask and purified by trypsinization and adherence repeatedly methods.

方法1。用组织块贴壁培养法获得原代卵巢癌CAFs和卵巢NFs，细胞铺满培养瓶后首次传代，通过胰酶消化法和反复传代法进行细胞的纯化；2。

Methods Forty pigmented rabbits were randomly divided into experimental and control group with 20 rabbits in each.

目的探讨玻璃体切割联合组织型纤溶酶原激活物和速避凝治疗细菌性眼内炎的效果。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。