组织原

Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物；培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感，据此可在传代过程中将二者分离纯化，获得纯细胞系；混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞；再加0.15%胰蛋白酶-0.02%EDTA 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞，经过2~3 代，即可得到纯化的乳腺上皮细胞系。

Fig.1 SHEE cultured on coverslide, the living cells were growing in single layer with rich cytoplasm, the nuclei were uniform in size with a nucleolus ph ×400 Fig.2 SHEE had a nucleus with ellipse shape, large nucleolus and the cytoplasm contained mitochondria and tonofibrilEM ×10 000 Fig.3 SHEE was spherical in shape, with pseudopods attached on petri dish and abundant villi on cell surface SEM ×5 000 Fig.4 Same as in Fig.3, cell attached on petri dish, appeared stellate or polygonal in shape, with abundant pseudopods and cytoplasmic processes. Protrusive nuclear region in central part of the cell had more micro-villi SEM ×5 000 Fig.5 Chromosomes of SHEE cells belonged to diploidy type Giemsa ×1 000 Fig.6 The SHEE cells of stained in dark brown by Ki67 immunohistochemistry were the proliferative cells Immunohistochemistry ×400 Fig.7 In SHEE cell culture, the nucleus stained red or pink by PI was dead cell, the green nucleus was living cell Fluorescent ×400 Fig.8 The cell labeled by TdT was apoptotic cell in which the chromatin of nucleus condensed in block, a pyknotic nucleus in the upper right conner was seen TdT labeled ×400

图1 SHEE培养在盖坡片上，活细胞单层生长，胞浆较丰富，细胞核大小一致，有核仁×400 图2 SHEE培养细胞细胞核椭圆形，核仁较大，胞浆有较丰富的线粒体和张力原纤维EM ×10 000 图3 SHEE细胞呈球状，有伪足贴壁，表面有密集微绒毛SEM ×5 000 图4 同上细胞贴壁，呈星状或多角形，有丰富伪足和胞浆突，核区隆起有较多微绒毛SEM ×5 000 图5 SHEE细胞染色体仍属二倍体Giemsa染色×1 000 图6 SHEE细胞Ki67免疫组织化学染棕黄色为增殖细胞×400 图7 SHEE培养细胞出现死细胞，胞核和胞浆PI染色呈红色或淡红色，蓝色细胞核为活细胞荧光显微镜×400 图8 细胞TdT标记阳性为凋亡细胞，染色质凝集呈块状，右上角有一固缩细胞核TdT标记×400

Sixty six male Sprague-Dawley rats were randomly divided into control group,and 5 model hepatic fibrosis groups perfused alcoholic,maize oil and pyrazole for 2 weeks,4 weeks,8 weeks, 12 weeks and 16 weeks (11 rats in each group) respect...

结果 与对照组比较，酒精灌胃 4周、8周、12周、16周组大鼠OPN、α SMA表达显著增强并且肝内胶原纤维含量显著增加。正常肝组织中几乎不表达OPN ，在酒精性肝纤维化大鼠模型的建立过程中，从第四周开始中央静脉。。。

The original Standards Code extended the reach of GATT law in two ways, both of which continue on as part of the WTO single undertaking: Firstly, it established a new legal theory for complaints based on 'unnecessary obstacle to trade' which focuses on the inherent trade restrictiveness of a regulation.

原标准法扩大了关贸总协定法两种方式，双方继续就作为世贸组织单一承诺：第一，它设立了一个新的法律理论投诉基础上的不必要的障碍，两岸经贸后者侧重于内在贸易限制性规定。

Dr. Sandrock said there is no vaccine that will protect against the virus, but he said that if populations are old enough to have experienced the 1968 strain, for instance, they may have a host-adapted MHC?I primed T-cell response with epitopes, is appropriate for viral clearance may confer some protection and result in a mild to moderate disease, where the host would just "feel crummy."

Sandrock医师表示，没有可以抵抗此病毒的疫苗，但他表示，如果一些年纪较大者、曾经历过1968年的病毒株，他们或许有抗原决定位的原发T细胞反应、宿主-后天主要组织相容性复体第一型，适合病毒清除以及赋予一些保护力，造成疾病较轻微或中等，宿主的感觉相当轻微。

Viral vector mainly contain adenovirus, retrovirus,adeno-associated virus and so on, but it has potential danger of safety; it isrepeled by immune system when it is injected to organism for a greatimmunogenicity. An injection with adenovirus vector with highconcentration, it leads to serious inflammatory reaction of liver. Viralvector such as liposome and polycation are commonly used lately. But,liposome and polycation have low specificness and targetness of genetransfer tissue, have lower transfection efficiency and short period ofgene expression, for they can be phagocytized by endothelial system.

许多的载体，病毒载体和非病毒载体以前已广泛应用，病毒载体主要包括腺病毒，逆转录病毒，腺相关病毒等；但病毒载体在安全性方面存在潜在的危险；免疫原性比较强，注射到机体后很快会被机体的免疫系统排斥，当静脉注射高浓度的腺病毒载体会使肝脏发生严重的炎症反应；非病毒载体目前常用的有脂质体及多聚阳离子聚合物；但脂质体和阳离子聚合物介导基因转移缺乏组织的特异性和靶向性，转染效率较低且易被网状内皮系统吞噬，基因表达时间短；因此研制新型的非病毒载体已成为研究的热点，纳米颗粒具有小尺寸效应，表面效应，随着颗粒直径变小，比表面积将会显著增大，故具有很高的化学活性，因而纳米成为了最有应用前景的非病毒载体。

In situ hybridization results showed that mouse Cdv-1 express only in spermatogenic cell in testis tissues.

在小鼠的睾丸组织中的原位杂交还显示：小鼠Cdv-1基因仅在精原细胞中表达。

Results:(1) The 90Y activity was higher while the contumination of 90Sr was less than 0.0031%;(2) The efficiency of 90Y-labeled antibodies was over 90%,the activity of labeled antibody maintained 82.3%;(3) Tumor size of all 90Y-antibody-treated model was significantly less than that of all 90Y-Cl3-treated model, the average survival time of the mice of the 90Y-antibody-treated group was significantly longer than that of the 90Y-Cl3-treated group;(4) In the tissue sections extensive necrosis was observed in all 90Y-antibody-treated tumor, the living tumor cells were immunohistochemically stainable with PcNA.

结果：(1)EDTA淋洗法制备90Y纯度高，其相对含量可达99.997％；(2)单克隆抗体的90Y标记率达90％以上，抗体蛋白活性保持82.3％；(3)治疗结果表明治疗组肿瘤明显小于对照组，原肿瘤的指数生长规律遭到破坏，治疗组生存期明显长于对照组；(4)组织学切片显示治疗组肿瘤有广泛坏死，PcNA免疫组化显示治疗组肿瘤细胞增殖受到明显抑制。

Urokinase, tissue -type plasminogen activator and plasminogen activator inhibitor-1 expression in severely stenosed and occluded vein grafts with thrombosis.

组织型纤溶酶原激活物在兔实验性肺血栓栓塞肺动脉中表达的动态变化及临床意义。

Stercobilin quantitative test: normal reference value of the average daily average of 20-125 mg, when the organization of hemolytic disease or bleeding, the fecal bile elements of the original emissions increase, suggesting that in vivo destruction of red blood cells increased significantly.

粪胆素定量试验：正常平均参考值平均每日20—125毫克，当溶血性疾病或组织内出血时，粪胆素原排出量增多，提示体内红细胞破坏显著增加。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。