组织原

The experiment study about the subcritical normalizing holding temperature effects the macrostructure and hardness of the 40SiMnCrNiMoA steel shows: When subcritical normalizing holding temperature is 760℃, the ferrite in the mixture phase structure is lumpish, the hardness of the steel is low (about HRC32), and when the holding temperature is over 800℃, the hardness of the steel is about HRC49, the ferrite in the mixture phase structure is in old critical range austenite grain boundaries, it is disadvantage for the steel strengthening. When the holding temperature is 780℃, the mixture phase structure is mixture phase structure including the strip ferrite、martensite and bainite, the ferrite is less than 20%wt, its grains are fine and even, the hardness of the steel is about HRC38, it is in the range of HRC35-40 which is the long-life drill rod needed.

亚温正火温度对40SiMnCrNiMoA的组织和硬度等性能的影响的实验研究表明，当亚温正火保温温度为760℃时，复合组织中的铁素体形态是块状，处理后的材料硬度相对较低（HRC32左右）；而当采用大于800℃温度亚温正火处理，材料硬度为HRC49左右，组织中铁素体大部分较连续地存在于原奥氏体的晶界，对材料强化不利；当40SiMnCrNiMoA钢780℃亚温正火后，硬度为HRC38左右，在以往小钎杆较长寿命所需硬度范围HRC35-40内，而组织为细条状铁素体+以板条马氏体和条状无碳贝氏体为主的复合组织，晶粒细小均匀，铁素体体积含量少于20％。

After 24 weeks,the operation area of group A was more smooth,and the surrounding normal cartilage naturally straight flush,transparent form new cartilage,subchondral bone formation in good condition;Group B restoration surgery the basic integrity of the cartilage tissue, center is not yet fully integrated,there was slight depression;CollagenⅡimmunohistochemistry of cartilage that was new brown area.Group C has no formation of articular cartilage.The growth and the intergration of subchondral bone of group A and B were better.

术后24周取材，见A组山羊手术区关节表面较为光滑，与周边正常软骨自然连续平齐，透明的新生软骨组织形成，软骨下骨形成完好；B组山羊手术区修复的软骨组织基本完整，中心部位仍未完全融合，有微小凹陷；Ⅱ型胶原免疫组化示新生软骨组织呈棕黄色。C组术区关节凹陷，无关节软骨组织形成。A组和B组，软骨下骨的生长及与周围组织的结合均较好，无植入物脱落现象的发生。

METHODS: The paraffin-embedded specimen of 95 cases of CRC, 57 cases of lateral tissues and 25 cases of metastatic lymphoid nodes were collected and constructed as tissue microarrays. The anormal expressions of EGF, EGFR, VEGF and COX-2 were evaluated by using the S-P method of IHC.

收集1997~1998年95例CRC原发灶、癌旁组织和转移淋巴结的石蜡标本，用组织阵列仪制备组织芯片；对组织芯片进行免疫组化染色，评估不同组织中EGF、EGFR、VEGF和COX-2的表达情况。

The biomechanical tests showed that two kinds of artificial bones had not significant difference on compressive strength and Young\'s modulus(P＞0.05),while the flexural strength of nano-nacre artificial bone was less than the control group(P＜0.05).3.The results of CCK-8 showed that the difference were not significant in each group,the proliferation of osteoblast reached the peak at the 5th day;7 days after being co-cultured,the total protein content of study group was higher than control group and blank group(P＜0.05),while the difference between control group and blank group was not significantP＞0.05The difference of alkaline phosphatase activities among three groups was not significant(P＞0.05The SEM view showed that osteoblast attached and grew well in two kinds of artificial bone.4.X-ray photography showed that two kinds of powder started to degrade in 2 weeks;this phenomenon became more appear in 4 weeks,nano-nacre powder degraded faster than micron-nacre powder,while the hole shadow was easy to be found;in 8 weeks,all the femoral holes recovered and returned to normal bone mineral density in all groups.Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone grew fastest around the bone defect area in study group,while most slowly in blank groupP＜0.05 SEM(scanning electron microscope observation showed that nano-nacre powder degraded more quickly.The same result can be found through the demineralized sections morphometric analysis,and both of the composite artificial bones made from those two kinds of nacre powder had the good connection with the adjacent tissue in rats body without apparent inflammatory response.5.X-ray photography showed that rabbit\'s bone defects healed faster in study group since NNAB implanted than in control group since MNAB implanted.At 24 weeks after operation,bone density in radial defects had nearly accessed to the normal area,while lower in control group,and turned up nonunion in blank group;The checking of BMD showed that results in study group were higher than those in control group at 8,16 and 24 week(P＜0.05), and the difference between the BMD values in study group at 24 week and those in blank group was not significant(P＞0.05).The gross specimens showed satisfactory histocompatibility both in study group and in control group,with bone tissue growing from two sides into the center of implanted materials; Normal slices in HE stain and hard tissue grinding slices in Stevenel\'s blue/Van Geison\'s picro-fuchsin stain showed that the bone growth tendency was better in study group than that in control group,and the medullary cavity had been penetrated to the implanted materials in study group at 24 week;Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone in both groups grew fastest 8 weeks after surgery,while slow down at 16 week.

纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨分别与成骨细胞共培养后，其各时间点CCK-8法检测值与空白对照无显著差异(P＞0.05)，成骨细胞均在第5天达到增殖高峰期；培养7天后，实验组细胞蛋白含量高于对照组及空白组(P＜0.05)，后两者之间则无显著差异P＞0.05碱性磷酸酶活性在三组间均无显著差异(P＞0.05电镜下可见成骨细胞在两种人工骨上都有良好生长贴附能力。4.X-ray显示两种粉体在大鼠股骨骨洞植入第2周时都开始出现了降解，第4周时更为明显，纳米珍珠层粉较之微米珍珠层粉降解更快，而空白对照组骨洞阴影仍可见，至8周时，则所有组骨洞均己闭合修复，X-ray下已不可见原钻孔痕迹，恢复正常骨质密度；硬组织磨片四环素荧光双标记结果显示纳米珍珠层粉植入组较其余两组在骨缺损区周围新骨生长速度更快，空白组速度最慢P＜0.05电镜观察及常规脱钙切片亦可见到纳米粉体降解较快；由以上两种原材料制得的纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨在大鼠体内均与周围组织结合良好，无明显炎症反应。5.X-ray显示纳米珍珠层/消旋聚乳酸复合人工骨植入兔桡骨缺损区后其骨愈合速度较对照组微米珍珠层/消旋聚乳酸复合人工骨植入的快，至植入术后24周，实验组骨缺损区接近正常骨密度，对照组骨缺损区密度较低，空白组则呈现骨不连状态；骨密度测量结果显示术后8周、16周、24周实验组的骨密度值高于对照组(P＜0.05，24周实验组的骨密度值与术前所测得的正常值无显著性差异P＞0.05动物取材大体所见均显示组织相容性良好，骨组织逐渐由植入材料两端向中央生长；常规切片HE染色及硬组织磨片Stevenel\'s blue/Van Geison\'s picro-fuchsin联合染色均可见实验组骨缺损区长势优于对照组，至术后24周，实验组骨髓腔与材料已呈相交通状；硬组织磨片荧光显微镜下观察，两组材料在术后8周处于骨生长最快速时期，16周时速度开始减慢，术后4、8、16周时实验组的新骨生长速度均较对照组的快

Result The optimum medium formula for protocorm propagation in tissue culture of C. grandiflorium was MS+0.1 mg/L 6-BA. The suitable medium for protocorm induction and differentiation in tissue culture of C. grandiflorum was WS+1.5 mg/L 6-BA+0.3 mg/L NAA with the bud-inducing rate up to 130%. The suitable medium for root inducement was 1/2MS+0.2 mg/L NAA+1.0 mg/6-BA and the survival rate of in vitro plantlets after transplanting was 78%.

结果］大花蕙兰组织培养原球茎粉殖以MS+0.1 mg/L NAA+2.0 mg/L6-BA培养基配方最好；适合大花蕙兰组织培养原球茎诱导分化的培养基配方为MS+1.5 mg/L6-BA+0.3 mg/L NAA，诱导出芽率可达130%；适合大花蕙兰诱导生根的培养基配方为1/2 MS+0.2 mg/L NAA+1.0 mg/L6-BA，试管苗移栽后成活率为78%。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

HE staining revealed that collagen fiber, fibroblasts and granulation tissue and synechia belt around tendons and stomas were apparently less in contrast to control group.

术后4周苏木精－伊红染色结果表明，实验组腱吻合口被胶原纤维连接，腱周及吻合口周围胶原纤维、成纤维细胞及肉芽组织明显较对照组少，腱周纤维组织较对照组粘连轻，粘连带稀少。

Materials and methods: The lung preparation of 6 SARS death patient (died in 9,14,20,29,33,38d) and 6 macaque model (killed in 7,12,14,14, 32, 35d)were objects. Pathological changes, collagen fibers, lattice fibers, elastic fibers, collagen I and III in lungs and fine structure changes were studied by routine H.E dyeing, trigeminy dyeing, trinitrophenol- sirius red staining and polarization microscope, electron microscope and image analysis. Expression of Vimentin、 TGFβ_1、 TNF α、 IL-1β and MMP-2 were detected by immunohistochemistry.Results:1. Pathological changes of SARS death patient.

材料与方法：以6例SARS死亡患者(分别于发病后9、14、20、29、33、38d死亡)和6只猕猴实验模型(分别于染毒后7、12、14、14、32、35d活杀取材)肺标本为对象，应用H.E染色、三联染色、苦味酸-天狼猩红-偏振光法、电镜和图像分析等技术，对比性观察SARS肺组织病理变化和纤维化的病理过程、胶原纤维、网状纤维和弹力纤维的变化特点、Ⅰ型与Ⅲ型胶原纤维的数量和分布规律，旨在探讨SARS肺纤维化的病变经历过程及特点；利用免疫组织化学和形态计量学技术检测SARS死亡患者肺脏的Vimentin、TGFβ_1、TNFα、IL-1β和MMP-2等与炎症反应和纤维化相关活性因子，探讨其发病机制。

Objective To research the influence of sun-exposure and ages to elastics and collagens of aging skins.

目的：探讨光曝露情况及年龄因素对皮肤中弹性组织、Ⅰ/Ⅲ型胶原及基底膜Ⅳ型胶原的影响，进而探索皱纹形成的组织学原因。

Results Astragaloside significantly lowered the mortality of DCM (35% vs 56%, P.05); PICP and PICP/PINP ratio were decreased in mice treated with astragaloside. Increase of CVF and Collagen I deposition in DCM was degraded in group Astr.

结果黄芪甲甙明显降低小鼠死亡率(35%vs 56%，P.05)；降低血清中胶原前肽PICP含量及PICP/PINP比率；组织病理学检测结果表明黄芪甲甙显著降低心肌组织中CVF及Ⅰ型胶原纤维的堆积。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。