组织原

In this article, we used automotive immunohistochemical stainer to check the expressions of HBsAg and HCV antigens in 100 cases of HCC and their adjacent tissues, mean while, we checked their histological activity index and hepatic fibrosis degree. We also used radioimmunoassay to detect serum levels of 4 serum fibrosis markers and serum AFP levels in these cases. Immunofluorescence labeling and flow cytometry were used to check the T-cell subpopulations and the activity of NK cells of these 100 cases. Then the correlations among these factors were studied.

本文采用全自动免疫组化仪对100例肝癌组织及癌旁组织中的HBsAg、HCV抗原表达进行了标记，同时对其进行肝组织活动指数、纤维化分期；同时采用放射免疫测定方法检测同期血清透明质酸、Ⅲ型前胶原蛋白、层粘连蛋白和Ⅳ型胶原蛋白等四项血清肝纤维化标志物和血清甲胎蛋白水平；采用免疫荧光标记技术和流式细胞仪对T细胞亚群及NK细胞活性测定，对各项指标的相关性进行系列对比研究。

The floral primordium is initially circular, along with the edge of which slight concaves occur. The outmost whorl of tepal primordia arises as a result of apical meristem division and the floral primordium then is in the shape of triangle. Two further whorls of tepal primordia arise in spiral acropetal sequence and are alternately arranged. When the innermost whorl of tepal primordia is at the late developmental stage, the foremost stamen primordia arise almost simultaneously in spiral acropetal succession and all of the stamen primordia are ultimately arranged in a triangle-cone form. Late in stamen primordium development, carpel primordia arise as same as the young stamen primordia and grow laterally. Grooves are present on the adaxial side of each carpel and develop into ventral sutures gradually closing up.

观光木的花原基最初为近圆形，随着顶端分生组织的活动，花原基边缘处出现浅凹，形成第一轮花被片原基，此时，花原基呈三角形排列，后两轮花被片原基依次发生，与前一轮互生；在内轮花被片发生的后期，最初几枚雄蕊原基几乎同时出现，呈螺旋状向顶发生，最后排列成三角圆锥状；雄蕊原基发育后期，心皮原基开始发育，形状与发育初期的雄蕊原基相似，随后心皮原基进行侧向生长，在近轴面出现浅凹，进而发育为凹槽，形成腹缝线，最后腹缝线完全愈合。

Results Same as hyaline cartilage, the calcified cartilage was stained light flavo-green and weak double refraction by the Picrosirius-polarization method. In the immunohistochemistry analysis of collagen Ⅱ protein, the calcified cartilage was stained strong green fluorescence and the collagen protein parallelled in joint surface near the tidemark. The content of collagen Ⅱ protein was (20.16%±0.96%) of tissue dry weight by amino acid analysis, and that of the hydroxyapatite was (65.09%±2.31%) of the inorganic composition in calcified cartilage.

结果 天狼猩红染色见钙化软骨层与透明软骨层一样黄绿色淡染、弱双折光性；Ⅱ型胶原蛋白免疫组织化学分析，钙化软骨层呈强绿色荧光，潮线附近的胶原蛋白呈板层平行分布；氨基酸定量分析钙化软骨层Ⅱ型胶原蛋白含量占组织干质量的（20.16%〖KG-*4〗±0.96%）；X-射线衍射定量分析钙化软骨层中的羟基磷灰石含量占无机成分的（65.09%±2.31%）。

Antibodies against col Ⅲ reacted strongly in the entire pterygial stroma. Conclusions Components of ECM are changed in the pterygium: the sub-epithelial connective tissues and vessels are more prominent in the pterygia than in normal conjunctiva tissues, and abnormal fibers are visible only in the deeper subepithelial connective tissues. This study documents the overexpression of LN and col Ⅲ. They may play roles in cellular adherency, migration and angiogenesis. Also this study documents the overexpression of MMP3 which may degrade ECM in the pathogenesis of the pterygium.

结论翼状胬肉组织中ECM的成分较正常球结膜发生改变，有大量的弹性纤维变性、胶原纤维增生及新生血管形成；col Ⅲ在翼状胬肉组织中表达增加，LN及胶原纤维可能对翼状胬肉中血管形成、细胞粘附和迁移及ECM重建起作用；MMP-3在翼状胬肉组织中表达增加，其对ECM成分的降解作用可能有助于翼状胬肉的形成和向角膜中央侵袭。

This in vivo study was to examine the historical changes of implanted novel chitosan/collagen composite barrier for confirming the clinical feasibility. Four other commercial GTR membranes were chosen for comparison. Among the resorbable GTR membranes, BioMend Extend and Peri-Aid are collagen base, and GORE-TEX OSSEOQUEST is synthesized membrane, while GORE-TEX e-PTFE (Expanded polytetrafluoroethylene) is synthesized but non-resorbable. Beagle dogs were used as animal model. Buccal mucoperiosteal flaps were reflected in the bilateral mandibular premolar and molar areas. Buccal alveolar bone was reduced on 1st、2nd premolar and molar to a level 5 mm apical to the cemento-enemel junction. Root surface was denuded of periodontal ligament and cementum, and notches were placed at the bone level of each root. The tested GTR barriers were implanted in critical bone defect areas. Flaps were coronally positioned and sutured. Two beagle dogs were sacrificed each time as the designed time period after surgery. Histological and histometirc evaluation at 7 days、14 days、28 days、3 months were performed post-operatively to determine the healing response of each treatment modality.

本研究之目的在评估与工研院生医中心合作发展出可吸收性去乙醯几丁聚醣/胶原蛋白复合之组织导引再生膜片，并选择与可吸收性之BioMend Extend 及Peri-Aid 胶原蛋白膜片，和GORE-TEX OSSEOQUEST 合成高分子膜片，及不可吸收之GORE-TEX e-PTFE合成高分子膜片，等其他四种市售商品材料共同对照评估，应用8只年龄为12个月的雄性小猎犬，均分4组(分别为7天、14天、28天、3个月)，为动物活体评估模式，在实验犬之左、右下颚第一、二小臼齿及大臼齿的颊侧区制造骨缺损后，分别植入组织导引再生膜片，依实验设定时间将小猎犬牺牲，取下缺损区骨头，以光学显微镜观察量测其牙垩质再生高度与齿槽骨再生高度之变化，以探讨其组织再生模式及评估新膜片在临床应用之适用性。

Methods Constructed the model of fetal scarless wound healing and investigated the expression of collagen in fetal and adult rat wounds by immunohistochemistry and in situ hybridization.

染色，Ⅰ、Ⅲ型胶原免疫组化，Ⅰ、Ⅲ型胶原原位杂交。结果胎鼠伤口愈合快，组织结构再生完全，没有瘢痕形成，胶原呈网状正常排列，Ⅰ、Ⅲ型胶原蛋白及表达均增多，同时伴细胞数增多，Ⅲ型胶原首先形成网状排列；成年伤口胶原纤维沉积慢，平行排列的胶原逐渐呈束状填塞伤口以致瘢痕形成，伤口内Ⅰ、Ⅲ型前胶原mRNA表达均增多，细胞数目增多不明显。

There was a significant inverse correlation between sarcomere length and A/I (r=-0.686, P.05). 4 Relations of the skeletal muscle histological characteristics and meat quality The muscle fibre and the myofibrils diameter were thinner, the muscle fiber density was denser, the sarcomere length was longer and the meat color was redder. The A/I was bigger, the meat color was whiter, contrarily was redder. The right amount of fat content may improve the meat brightness. The muscle fiber diameter and the myofibrils diameter were the biggest factors to influence the skeletal muscle shearing force, their assumed the obvious linear correlation. The muscle fiber density influence on the shearing force was only inferior to the muscle fiber and myofibrils diameter. Mensurate the IFA from tectology angle may reflect in intramuscular fat content. However intramuscular fat was not enough to carry weight on the shearing force, it may mostly affected on the smack, but not the tenderness expression.

猪骨骼肌组织学特性与肉质性状的关系肌纤维和肌原纤维直径越小、肌纤维密度越大、肌节长度越长，肉色鲜红；肌节中暗带比例越大，肉色偏白，而明带比例大，肉色偏红；适量的脂肪含量可以改善肉的亮度；肌纤维直径和肌原纤维直径对骨骼肌剪切力的贡献率最大，呈明显的线性相关，肌纤维密度对剪切力的贡献率仅次与肌纤维和肌原纤维直径；从组织形态学角度测定肌内脂肪面积比一定程度上可以反映肌肉内的脂肪含量，而肌内脂肪对肌肉剪切力的贡献率比较微弱，对肉食用品质的影响可能更多表现在改善肉的风味，而不是嫩度的表达。

They were composed of wavy and tendinous collagen fiber fascicles, among which a small amount of loose tissue was found to be intermingled with vessels.

桡舟韧带、桡舟头韧带和桡月韧带的组织结构相似，由肌腱样的胶原纤维束组成，胶原纤维束之间有少量的疏松组织，其间有血管分布，胶原纤维束呈小的波浪状。

Whereas a cultivated land which has been occupied for non-agricultural construction upon approval and can sure start construction within one year is found cultivable and yieldable, it should be cultivated by the unit or individual that originally cultivates the land or cultivated by units occupying the land.

已经办理审批手续的非农业建设占用耕地，一年内不用而又可以耕种并收获的，应当由原耕种该幅耕地的集体或者个人恢复耕种，也可以由用地单位组织耕种；一年以上未动工建设的，应当按照省、自治区、直辖市的规定缴纳闲置费；连续二年未使用的，经原批准机关批准，由县级以上人民政府无偿收回用地单位的土地使用权；该幅土地原为农民集体所有的，应当交由原农村集体经济组织恢复耕种。

Article 37 All units and individuals shall be prohibited to let cultivated land lie idle or make it barren.

第三十七条禁止任何单位和个人闲置、荒芜耕地。已经办理审批手续的非农业建设占用耕地，一年内不用而又可以耕种并收获的，应当由原耕种该幅耕地的集体或者个人恢复耕种，也可以由用地单位组织耕种；一年以上未动工建设的，应当按照省、自治区、直辖市的规定缴纳闲置费；连续二年未使用的，经原批准机关批准，由县级以上人民政府无偿收回用地单位的土地使用权；该幅土地原为农民集体所有的，应当交由原农村集体经济组织恢复耕种。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。