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P1 BMSCs, P3 CCs, and P3 ADAS were implanted into collagen/hyaluronic acid scaffold at different concentrations of 10%, 20%, 50%, 80%, and 100%, and the cells were then three-dimensionally cultured in serum-free culture media. Two weeks later, SO staining and immunohistochemical staining were employed to observe the synthesis between of chondroitin sulfate and type II collagen.

取P1骨髓间充质干细胞,P3软骨细胞和P3脂肪源性成熟基质细胞种植入不同比例(10%,20%,50%,80%,100%)的胶原/透明质酸支架中,在无血清培养液中三维培养。2周后,用SO染色和免疫组织化学染色分析,观察硫酸软骨素和Ⅱ型胶原合成情况。

Because people sclera is composed of collagen, while the body's iron is an important collagen synthesis cofactor.

因为,人的巩膜是由胶原组织组成,而体内的铁元素又是合成胶原时的重要辅助因子。

BACKGROUND : By now , safety of collagenase application is still controvertible and some scholars believed that collagenase might induce the peripheral tissue injury .

背景:目前对于胶原酶应用的安全性仍有不同观点,有学者认为胶原酶可能会对周围组织产生损伤。

The distribution of ABH substances in humantissue cells of nonsecretors differs from those insecretors.the contents of ABH in cells of nonsecretorswere much less than those of secretors.ABH substanceswere demonstred in cells of deep part of gastric gland,Brunner gland of duodenum,acini and duct of pancreas,acini and duct of sweat gland and in endothelial cells ofblood vessels and heart.

在国内外首次用免疫透射电镜对正常人体的胃、十二指肠、横结肠的粘膜上皮和腺上皮的ABH物质进行亚细胞定位研究后发现,ABH物质分布于粘膜上皮及粘液性细胞的Golgi器、粘原颗粒和胃腺主细胞的酶原颗粒中,证实人体组织细胞的ABH物质由自身产生,而不是从血液吸附。

Different types and levels of development of the countries generally have taken a different political systems, but may also in some ways have the same or similar system; the same type and level of development of the countries generally have the same or similar political systems, but in some areas there may be very different:(1) according to state management form, namely the form of state power points, there is absolute monarchy system of slave society, the nobility republican, democratic republic; State has the absolute monarchy of feudalism, class representative of the monarchy, republican (little city-state has adopted); capitalist countries have constitutional monarchy and democratic republic two types of constitutional monarchy is divided into the dual monarchy such as the Hashemite Kingdom of Jordan, and a parliamentary monarchy like the United Kingdom, democratic republic is divided into parliamentary cabinet system, the presidential system and the committee system in three forms; of the socialist countries of the Soviet system of the former Soviet Union, former Yugoslavia, the delegation system, China and Cuba and other countries of the people's congress system and so on.

不同类型和发展程度的国家一般都采取不同的政治制度,但也可能在某方面有相同或相似的体制;同一类型和发展程度的国家一般都有相同或类似的政治制度,但在某些方面也可能有很大差异:(1)按国家管理形式即政权组织形式分,奴隶社会有君主专制制、贵族共和制、民主共和制;封建主义国家有绝对君主制、等级代表君主制、共和制;资本主义国家有君主立宪制和民主共和制两大类型,君主立宪制又分为二元君主制如约旦哈希姆王国和议会制君主制如英国,民主共和制又分为议会内阁制、总统制和委员会制三种形式;社会主义国家有原苏联的苏维埃制,原南斯拉夫的代表团制,中国和古巴等国的人民代表大会制等。

Results Osteoclast and osteoblast around trabecula of bones showed proliferation, shi1e fatty tissues in bone marrow decreased and hematopoietic tissue well-regenerated alter the hyperbaric oxygen-treated. Also juvenal osteocyte and collagen fibril were observed in early stage by electron microscope.

结果 多数骨小梁周围可见骨母细胞增生、偶见破骨细胞增生,髓腔内脂肪细胞逐渐减少,造血组织增生;电镜发现骨小梁内早期即出现幼稚骨细胞和胶原原纤维合成。

OBJECTIVE: Using membrane guided tissue regeneration technique, to observe the healing velocity and quality of repairing rabbit radius segmental defect combined collagen membrane with allogeneic bone, further more, to contrast the result with pure collagen membrane.

目的:利用膜引导组织再生技术,观察胶原膜复合同种骨颗粒修复兔桡骨节段性骨缺损的成骨速度和质量,并与单纯胶原膜进行比较。

Cells of the procambium and ground meristem are more vacuolated than those of the protoderm.

原形成层和基本分生组织的细胞比原表皮层细胞更加液泡化。

Basing on the nectary structure and polysaccharied changes, it was supposed that storing of per-nectar and decomposing of polysaccharide were conducted in nectariferous tissue. The epidermis was the main place where processing of per-nectar and synthesizing and secreting of nectar were conducted.

根据花外蜜腺的特点及多糖的变化分析,原蜜汁的贮存及多糖的降解主要在产蜜组织细胞中进行,而分泌表皮细胞则是原蜜汁的加工及蜜汁的合成与分泌的主要场所,且分泌细胞内含有浓厚的细胞质,有显著的细胞核和大量的细胞器。

Nighty rats were randomly divided into the groups of control, myocardial infarction and doxycycline, which were given orally 48 hours before and 48 hours after MI with 30mg/Kg per day. Protein and mRNA extraction was done on left ventricular samples containing scar and myocardium together. Samples were assayed from 1 day to 4 weeks post-Mi. The activity of MMPs was measured by zymography and collagen amount by the method of chloramine T, the ratio of II III collagen was assessed by immunohistochemical stain. Protein and mRNA of MMP-2, 9, TIMP-1 were determined by Western-Blot and RT-PCR.

分为对照组、心梗组和治疗组(D组,术前两天至术后两天,口服Doxycycline,30mg/kg/天),分别于术后第一天、术后一周、术后两周、术后四周取心肌组织,采用免疫组化测定胶原含量和Ⅰ/Ⅲ胶原比例,酶谱法测定心梗后MMP-2,9活性蛋白的表达规律,Western Blotting进一步确定酶谱法中所消化条带蛋白的属性,逆转录-聚合链反应法测定心梗后MMP-2,9和TIMP-1的mRNA的变化规律,运用Acuson Sequoia 512超声心动图机分别测定心梗组、治疗组在两周和四周时左室前壁、后壁厚度以及左心室舒张末内径和左室射血分数。

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