组织化学的

The lesion, the changes of activity of cholinesterase and acid phosphatase of motor neurons in lateral nucleus of spinal cord anterior horn, the function of axoplasm transportation and nerve conduction, the regeneration of axons and myelin sheath, and the recovery of sciatic nerve function were examined at 7, 14, 30 and 90 days intervals after operation, using Nissl and enzyme histochemistry staining, electrophysiological technique, hydrogen-peroxide oxidoreductase retrograde trace method, axon image analysis, and measurement of sciatic function index. The spinal cord anterior horn of injury side was compared to the correspondence region of spinal cord.

分别于术后7、14、30和90d应用酶组织化学方法、电生理方法、HRP （hydrogen-peroxide oxidoreductase）逆行示踪方法、轴突图象分析方法以及坐骨神经功能指数（sciatic nerve function index，SFI）测量等方法检测坐骨神经损伤后对应脊髓神经元的存活率、神经元胞体酶系变化、损伤神经在轴浆运输、电传导以及轴突、髓鞘再生等方面的恢复情况，探索外周神经损伤后OECs及几丁质对神经元的保护作用以及对新生神经功能恢复的作用，为外周神经损伤的治疗提供新的理论基础。

The effects of FPL andits three ingredients on growth of ovary,on micro-and ultra-structure andAlkaline phosphatase activity of endometrium,on structure andkeratinization rate of exfoliated cells in vaginal epithelium in mice,onmicrocirculation in rat and on uterine kinesis in rabbit were respectivelyobserved by the techniques of light and electron microscopes,histochemistry,microcirculation and organ cinetography.The results have confirmed themechanism of FPL for the sterility caused by ovary standstill and persistentcorpus luteum to be as follows:(1) a weak estrogen-like role which canprompt development of ovary,proliferation and secretion of endometrium andvaginal epithelium and cornification of exfoliated cells in vaginal epithelium;(2)enhancing AKP's activity of endometrium;(3)improving microcirculation;(4) exciting uterus.

分别采用光学显微镜和电子显微镜技术、组织化学技术、微循环技术和器官运动描记等现代医学实验技术，观察了促孕液对小白鼠的卵巢发育、子宫内膜的显微和超微结构和碱性磷酸酶活性、阴道上皮结构和脱落细胞的角化率、对大白鼠的微循环和对家兔的子宫运动的影响，结果证实了促孕液治疗卵巢静止、持久黄体的机理：(1)微弱的雌激素样作用，能刺激卵泡发育，促进子宫内膜和阴道上皮增生、分泌和阴道上皮脱落细胞角化；(2)提高子宫内膜碱性磷酸酶的活性；(3)改善微循环；(4)促进子宫运动。

This study that observed the expression and distribution of 4 apoptotic factors, relation apoptotic factor with apoptosis, changes of T and B subgroup, and changes of molecular pathology in 14 cows suffering from enzootic bovine leucosis from several resides was detected by histochemistry, TUNEL technology, immune-fluorescent flow cytometry and so on. The aim of this experiment supplies theoretic basis for preventive and therapy of EBL, and consult dates for T lymphoid cell leucosis of human being .

本研究检查并剖检了14例临床与实验室诊断为EBL的病牛，观察分析了肿瘤发生的情况和特征，采用免疫组织化学ABC法、原位末端标记TUNEL技术和免疫荧光流式细胞检测法等技术，从EBL肿瘤细胞凋亡特性入手，围绕4种凋亡因子和几种免疫相关因子与EBL肿瘤发生的关系及其在诊治方面的应用进行了首次有价值的探索，其目的在于为防治EBL提供科学的理论依据，同时，也将为人类T细胞白血病Ⅰ。

Rat AAA model was made by perfusion PPE elastase through the abdominal aorta, and the specimen was obtained on postoperativeday 3, 7, 14, 28, respectively. Specific elastic fiber staining was used to observe the disruption of elastic fiber in aortic wall. Immunohistochemistry staining of CD68 was applied to observe the microphage infiltration. The in tisu hybridization and western blot of MCP-1 and MMP-2 were used to study the expression of mRNA and protein. Thus we can analyze the facilitated function of MCP-1 gene to the microphage adherence and infiltration on the earlier AAA.

采用经腹主动脉腔内导管灌注的方法制成大鼠AAA模型，分别于术后3天、1周、2周、3周、4周获取各组大鼠腹主动脉标本，行弹力纤维特殊染色观察动脉壁弹力纤维的破坏情况；行CD68免疫组织化学染色观察动脉壁中巨噬细胞的浸润；行MCP-1及MMP-2的原位分子杂交、Western蛋白质印迹观察二者的mRNA表达和蛋白表达；分析MCP-1基因促进巨噬细胞的黏附、浸润在早期AAA发病中的作用。

Which may be useful in the study of insulin signaling transduction pathway in the brain.methods:use immunohistochemical techniques to investigate the neuronal distribution of insr、irs-1 and irs-2 proteins in rat brain with polyclonal insr、irs-1 and irs-2 antiserum.results:insr、irs-1 and irs-2 are widely distributed in the brain of normal adult rat ,especially in the cerebral cortex 、hippocampus and numerous hypothalamic nuclei including the arcuate nucleus、ventromedial nucleus and paraventricular nucleus which are crucial for the control of food intake.conclusion:insr、irs-1 and irs-2 are widely distributed in the brain of normal adult rat, suggesting that they may play an important role in the normal function of central nervous system.

应用免疫组织化学染色的方法观察正常成年大鼠脑中insr、irs-1、irs-2的分布。结果：insr、irs-1、irs-2在正常成年大鼠脑中广泛分布，在与学习记忆和能量代谢相关的脑区如大脑皮质、海马、丘脑和下丘脑的部分核团均有表达。结论：insr及irs-1、irs-2在正常成年大鼠脑中广泛表达，但表达部位不完全一致，提示它们在大鼠中枢神经系统的正常功能活动中可能发挥不同的作用。

The journal publishes reviews covers the whole field of the biology and biochemistry of cancer, emphasizing oncogenes and tumor suppressor genes, growth-related cell cycle control signalling, carcinogenesis mechanisms, cell transformation, immunologic control mechanisms, genetics of human cancer, control of cell proliferation, genetic and molecular control of organismic development, rational anti-tumor drug design.

该杂志主要发表有关肿瘤生物学和生物化学的综述。其内容包括：致癌基因和肿瘤抑制基因，与生长有关的细胞周期控制的信号转导，致癌机理，细胞转化，免疫控制机理，人类癌症遗传学，细胞增殖的控制，组织发育的遗传和分子控制，合理的抗肿瘤药物设计等。

The results indicate that the longitudinal nerve fibers in cerebral ganglia and a few of small cells in the surface layer of cerebral ganglia present NOS positive reaction. Abundant NOS positive small cells are in the surface layer of pedal ganglia, and abundant transverse positive nerve fibers in the center of pedal ganglia. A large number of transverse positive nerve fibers are in the center of visceral ganglia; abundant positive small cells and nerve fibers are in two anterior lobes; a few of positive small cells and many encircled positive nerve fibers are in the posterior lobe; a large number of radiate positive nerve fibers are in the lateral lobes.

组织化学显示，存在NOS的部位如下：脑神经节内纵行的神经纤维和表层的少量小细胞；足神经节表层的大量小细胞，中央大量水平分布的神经纤维；脏神经节中部大量水平分布的神经纤维，前叶内大量小细胞和神经纤维，后叶内少量小细胞和许多环行神经纤维，侧叶内大量似放射状分布的神经纤维；脑足和脑脏神经索内的神经纤维。

Methods: Neuroepithelial stem cells derived from the embryonic neural tube of GFP transgenic mice were cultured and depurated in free serum medium containing B27, bFGF and EGF, and then they were directionally transplanted into the striatum of rats.

将GFP转基因鼠的胚胎神经上皮干细胞置于无血清培养基内纯化扩增，用巢蛋白免疫组织化学染色鉴定神经干细胞，将扩增后的含GFP基因的神经干细胞在立体定位仪下，定向移植到大鼠的纹状体内。

Methods: The study was designed to observe SS and influence of serum CD_4~+、CD_8~+、IL-2、IL-6、TNF-α, splenic lymphocyte transformation rate, the expression of SS、SSTR-2 in spinal cord、PAG、NRM and hypothalamus the coexistence of SS and enkephalinergic neuron in back corner of spinal cord, the coexistence of SS and 5-HT neuron in NRM by using methods of behavioral nociceptive threshold test, measure of foot's cubage, flowcytometry, MTT, radioimmunity, HPLC-ECD, immunohistochemistry, laser confocal microscope on FAC-induced hyperalgesia.

以弗氏完全佐剂诱发的炎症痛模型大鼠为研究对象，应用行为学痛阈观察、足跖容积测定、流式细胞检测法、MTT法、放射免疫法、高效液相电化学法、免疫组织化学技术法、激光共聚焦技术等方法，观察SS和电针对痛阈、大鼠CD_4~+、CD_8~+、IL-2、IL-6、TNF-α水平和脾淋巴细胞转化率等指标的影响，以及SS、SSTR-2在脊髓和PAG、NRM及下丘脑部位的表达、在脊髓SS与脑啡肽能神经元的共存现象及在NRM中与5-HT能神经元的共存情况。

For the purpose to study its critical issues for fusion application, many kinds of V-W-Cr-Ti-Al alloys were developed, and were used for studies of recovery and recrystallization, aging behavior and precipitation hardening, high-temperature mechanical performance, effects of H on mechanical properties and hydrogen enbrittlement, H release behavior and oxidation behavior at elevated temperature. Mechanizms for the behaviors were analyzed and the relationship between chemical composition, microstructure and properties was established.

为研究其聚变应用关键问题，本项目研制了多种V-W-Cr-Ti-Al合金，利用它们完成了合金的回复和再结晶、时效行为和析出强化、高温力学性能、氢对合金力学性能的影响及氢脆、氢释放行为、高温氧化行为等研究，分析了合金的行为机理，建立起了合金性能与化学成份、微观组织结构的关系，是以后研制更高性能合金的基础。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。