组织化学的

The Immunohistochemical results indicated that the pI〓 is obviously located on the liminal surface membrane of the distal tubules and Henle's ascending limbs, and can also be found at Henle's loop of porcine kidneys, these segments expose in non-isotonic condition.

免疫组织化学染色显示，pI〓在猪肾脏明显定位于非等渗区域的远曲小管和髓袢升支粗段的管腔表面膜及髓袢处，而等渗区域的近曲小管细胞几乎无pI〓的存在，据此认为pI〓可能在耐受渗透压的变化上发挥作用。

C2C12 cells were induced to differentiation into muscle cells in vitro and the myogenesis model was detected by inverted microscope, RT-PCR, western-blot, cell immunofluorescence histochemistry, flow cytometry and confirmed to be successful. On the foundation of differentiation model, miR-206 was detected by northern hybridization and the result showed that the expression level increasing gradually during muscle differentiation with a character of phase specificity.

利用C2C12细胞在体外成功诱导其向成肌细胞分化，通过形态学观察，RT-PCR、western-blot、细胞免疫荧光组织化学及流式细胞仪等方法进行检测，证实我们所建立的细胞分化模型是成功的，并观察到随着诱导时间的延长，miR-206在成肌分化过程中表达量逐渐增高的，表达改变具有阶段特异性。

It is a very important question for understanding the integrative processes of RVLM neurone taking part in the cardiovascular activity under different situations.

这些不同形态和组织化学不同的神经元，可能是它们的在心血管活动中作用不同的原因，它们与诱发放电类型的关系有待进一步探讨。

Results There was significant negative correlation between the Nogo-A expression and the morphological change of tumor tissues according to immunohistochemistry. Western-blot analysis also indicated that the gray value of Nogo-A protein band in the oligodendroglioma group was significantly higher than that in the anaplastic oligodendroglioma group.

结果 免疫组织化学结果显示，Nogo-A免疫阳性染色的光密度值与肿瘤细胞核分裂、核异型比例呈明显的负相关，对蛋白印迹试验结果进行光密度分析也发现少突胶质细胞瘤中的Nogo-A蛋白条带灰度值明显高于间变型少突胶质细胞瘤中的灰度值。

The Research of the equine diseases and the Analysis of their reasons in Henan Province;2. Epidemiological investigation about equine tumors in Gansu province;3. The results showed that prepubertal orchidectomy of the equine resulted in a slight but not significant increase in the number and percentage of gonadotrophs,but significantly increased the average area of .

利用免疫组织化学和形态计测学的方法，观察了 7匹公马和 6匹在 1～ 2岁期间被摘除了睾丸的阉割马的脑垂体前叶促性腺激素细胞的数量和面积，同时利用放射免疫分析方法检测了血浆中垂体促卵泡激素和垂体促黄体激素的水平。

Methods Endogenous EB1 mRNA and protein levels in embryonic cells of trophonema from artificial abortion and spontaneous abortion were compared, using fluorescence quantitative PCR, immunohistochemistry and western blot, respectively.

采用荧光定量PCR技术检测EB1基因在人工流产绒毛核型分析正常的胚胎细胞和自然流产绒毛核型分析异常的胚胎细胞中的表达差异；免疫组织化学方法检测EB1蛋白在两者中的表达情况，并用Western blot加以验证。

The distribution of fibronectin in human oocyte and 2-cell to morulae embryos was also investigated, and the possible roles of fibronectin in fertilization, the development of early embryo and implantation were also discussed.

应用免疫组织化学方法和图像分析技术研究整合素β_3、FN和ER、PR在输卵管粘膜内的表达和变化；采用间接免疫荧光技术对FN在人卵母细胞和早期胚胎内的表达分布进行观察；光镜观察输卵管粘膜上皮细胞的形态学变化。结果：1。整合素β_3在人正常输卵管粘膜上皮纤毛细胞的胞浆内表达。

Methods: In order to discuss the JIANGU granule treatment GIO molecular action mechanism, this research takes the osteoporosis sickness animal experimentation model by the glucocorticoids-induced induction domestic rabbit, through HE dyeing research Chinese native medicine JIANGU granule to vacancy bone sunken number influence; Through the immunity histochemistry technology,observes the blood vessel bast growth factor in the bone tissue expression. Through home position hybrid technology,in mRNA member level research, collagen I mRNA distribution and expression.

本研究以糖皮质激素诱导的家兔作为骨质疏松症动物实验模型，通过HE染色研究中药健骨冲剂对骨组织的空缺骨陷窝数的影响；通过免疫组织化学技术，观察血管内皮生长因子在骨组织的表达；通过原位杂交技术，在mRNA分子水平研究，Ⅰ型胶原mRNA在骨组织中分布及表达。

Histochemical assays of GUS gene showed those resistant callus and roots from transgenic plants cultured under light were no blue-stained with X-gluc, but callus and roots after 24 h treatment of darknees and then incubated with X-gluc could present blue. Fluorimetric enzyme quantitative analyses indicated GUS expression in transgenic plants was organ specificity.

转基因抗性愈伤和转基因植株根的组织化学GUS活性检测表明，光照培养下的抗性愈伤和转基因植株根不能使X-gluc显色，而暗处理24 h后的抗性愈伤和定植后转基因植株的根能使X-gluc显色。

To answer these questions, in the present study, an attempt was made to examine:(1) the GABA responses in mechanically dissociated Vme neurons with attached synaptic boutons by using the whole cell patch clamp technique;(2) the change of expression of KCC2 and NKCC1 during the postnatal stage in the Vme by using the Western blotting technique;(3) the percentage of colocalization of PV with KCC2 or NKCC1 during the postnatal stage in the Vme by using the double immunofluorescence histochemical techniques.

基于此，本论文进行了如下研究：(1)运用机械分离带终扣的单细胞膜片钳技术对大鼠Vme神经元对GABA的反应进行观察；(2)运用Western blotting技术结合图像分析方法观察生后发育过程中Vme神经元内NKCC1和KCC2的表达变化；(3)运用免疫荧光组织化学双重标记技术对生后发育过程中Vme神经元内NKCC1和KCC2与PV之间的共存关系进行了系统观察。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。