组织化学的

In Pteridaceae s. l., false veins can be found in Pteris, Adiantum, and vittarioids; in this thesis, Energy Dispersive X-ray element analysis and histochemical studies were used to confirm the false veins of these three taxa are composed of spicular cells – a kind of long, silica depositing epidermal idioblast.

於广义的凤尾蕨科中，假脉可见於凤尾蕨属、铁线蕨属以及书带蕨类群；本论文利用能量色散X光光谱仪元素分析，配合组织化学染色的结果，证实此三类群的假脉均是由具二氧化矽堆积的矽异形细胞(spicular cell， SC)所构成。

In the brain of adult rat, the positive immunohistochemical product of lSL-l (ISL-l-positive) was mainly located in the neuronal nucleus and found in discrete regions except to brain cortex, such as the Purkinje cell layer and the granular cell layer of cerebellum, the granular cell layer and the pyramidal cell layer of hippocampus, the mitral cell layer, the internal and external plexiform layer, the granular cell layer and the granular cell layer of olfactory bulb and so on, and several nuclei of the hypothalamus, midbrain and pons, such as claustrum, anterior olfactory nucleus, accumbens nucleus, caudate-ptamen, pallidum, substantia nigra, striatum, islands of Callaje, mammillary nucleus, anterior pretactal nucleus, habenular nucleus, amygdaloid nucleus, cuneate nucleus, rubral nucleus, gigantocellular reticular nucleus and so on.

在正常成年大鼠脑中，同源框基因islet-1表达产物（ISL-1）免疫组织化学阳性物质广泛分布于除大脑皮层外的神经细胞的细胞核内，ISL-1阳性神经元密集分布于小脑Purkinje细胞层和颗粒细胞层、海马的颗粒细胞层和锥体细胞层、嗅球的内丛层、外丛层、颗粒细胞层及僧帽细胞层等，另外在丘脑、中脑和桥脑的一些重要神经元核团均有分布，如，屏状核、前嗅核、伏核、尾壳核、苍白球、黑质、纹状体、Calleja岛、乳头体核、前顶盖前核、缰核、杏仁核、楔束核、红核网状巨细胞核等。

Counterirritant chemicals produce reversible, transient irritation or mild inflammation of the surface tissues following application.

抗刺激化学产品的产生是组织表面的短暂的刺激或轻微的炎症产生以下可逆的一系列程序。

EDD and NEDD of external iliac artery in vivo (measured with high-resolution ultrasound), serum lipids, NO, NOS, plasma ET, ADMA, symmetric dimethylarginine and L-arginine, the area of AS plaque in thoracic aorta (dyeing with Sudan Ⅳ), the degree of AS lesion in abdominal aorta and external iliac arteries, NOS activity on the wall of the abdominal aorta, the expression of iNOS mRNA and eNOS mRNA on the wall of the abdominal aorta and the contractive and dilatant functions of abdominal aorta and external iliac arteries in vitro; B. the activities of dimethylarginine dimethylaminohydrolase in kidney (including the establishment of the determining method). 3. In the third part of the study, normal aorta strips were used. The following were surveyed: their response to using ADMA alone, influence of treating with ADMA, intervention of polydatin, influence of using ADMA and PD together on Emax and Kd that were contracted by PE.

第5组为未给药组。2、观察指标：A、各组髂外动脉EDD和NEDD、血脂、血清NO和NOS活性、血浆ET、ADMA、SDMA和L-精氨酸水平、胸主动脉AS斑块面积、腹主动脉和髂外动脉AS病变积分、腹主动脉壁NOS组织化学染色和eNOS mRNA和iNOS mRNA表达及离体腹主动脉与髂外动脉舒缩功能。B、各组兔肾DDAH活性。3、离体做正常主动脉条对单用ADMA的反应、PD和ADMA对PE收缩效应的影响及PD预处理对受ADMA作用的正常主动脉条PE收缩反应的影响(包括对Emax和Kd值的影响)。

Although many efforts have been made to elucidate the role of Kail gene in many kinds of carcinoma,there are little reports concerning the role of Kail in human colorectal carcinoma,especially regarding the mechanism of its down-regulation in metastasis carcinoma. In present study,the expression of Kail was detected by in situ hybridization,immumohistochemistry and Western Blot in four cell lines of HCC with different metastatic potential.

为探讨Kai1基因与大肠癌转移之间的相关性，本研究利用四种不同转移潜能的大肠癌细胞系HCT116、HT29、LoVo与SW480，从mRNA与蛋白质水平，用原位核酸杂交、免疫组织化学与Western Blot三种方法检测Kai1／CD82的表达，得到了一致的结果：Kai1／CD82在HT29与SW480细胞系的表达较LoVo与HCT116为高。

Methods ①In situ hybridization was applied to observe the changes ofhypothalamic CRHmRNA positive cells after PX and melatonin supplementation;②Immunohistochemistry was used to observe the changes of CRH positive cellsin hypothalamus and CRH staining in median eminence of neurohypophysis after PXand melatonin supplementation;③ELISA was applied to detect the changes of CRHconcentration in serum after PX and melatonin supplementation.

①运用CRHmRNA 原位杂交的方法来观察松果体摘除及补充外源性褪黑素后下丘脑室旁核CRHmRNA 阳性细胞的变化；②应用免疫组织化学染色的方法观察松果体摘除及补充外源性褪黑素后下丘脑室旁核CRH 阳性细胞和神经垂体正中隆起CRH 阳性染色的变化；③运用ELISA 法检测松果体摘除及补充褪黑素后血清CRH 浓度的变化。

In the positive group of the micrometastasis, the difference was significant statistically ( p 0. 01). There was significant difference of the recurrence rate between the two groups (p .01).ConclusionHie method of conjointing the intermission - continuous - section and CK20 immunohistochemistry technique may be valuable to re-estimate the LN metastasis status and stage of colorectal cancer and predict the prognosis.

1、间断切片法结合CK20免疫组织化学方法有助于检出Dukes B期大肠癌淋巴结微转移，增加每个淋巴结的切片数量有助于提高微转移的检出率； 2、Dukes B期大肠癌低分化及癌肿侵犯肠周径＞3／4者易发生淋巴结微转移； 3、淋巴结微转移阳性组与阴性组的生存率与复发率有显著差异

Objective: To observe the distribution pattern and morphology of NOS positive neurons in the olfactory bulb of the wild hedgehog .

用依赖还原型辅酶Ⅱ的黄递酶组织化学方法显示NOS阳性神经元在刺猬嗅球的分布和形态，观察野生刺猬嗅球内一氧化氮合酶阳性神经元的分布和形态。

"This advance is exciting because the physical nature of this technique allows the production of very long amorphous nanofibres with tailored compositions," lead author Félix Quintero told nanotechweb.org.

研究人员指出，这项利用物理而非化学的技术可以制作出非常长且成份经设计的非晶型纳米纤维，可能的应用包括纳米复合材料、纳米模版(nano- templating)、组织工程、感测器及新型织物。

Results 1、 Generally, we can see the original blue and white, shiny, no cracks in the articular surface of the cartilage after the stress increases gradually yellow, surface roughness, cracks appear; when the pressure decreases, the yellowing, rough, the color of the fracture restore gradually and become shiny.2、the shiny smooth surface can be seen under a light microscope, formation, cell distribution, tidy, clear the level of cartilage at the articular surface stress increases, the surface roughness changes, defects, disordered cells, uneven dyeing ; when the articular surface of the pressure gradually decreased, the cartilage gradually repair and the surface of cells at the surface appear only disorder.3、immunohistochemical observation can be seen throughout the observation period, cartilage cells are type Ⅱ collagen expression and expression after 3 weeks gradually weakening, when the seventh week begin to strong gradually.4、 electron microscopy shows that when stress increases the articular surface, the cartilage cells became flat, the cytoplasm in the endoplasmic reticulum, Golgi apparatus decreased with collagen disorders; and when stress decreases the articular surface, cartilage cells gradually returned normal, cytoplasm in the endoplasmic reticulum, Golgi body gradually restore quantity; collagen fibers with a gradual rules.

结果：①大体观察可见到原本蓝白色、有光泽、无裂纹的软骨在关节面压力增大后，逐渐呈灰黄色，表面粗糙，出现裂隙；当压力逐渐减小后，变黄、粗糙、有裂隙的软骨颜色逐渐恢复，变得有光泽②光镜下可见表面光滑、平整，细胞分布均匀、整齐，层次清楚的软骨在关节面压力增大后，表面变粗糙、缺损，细胞排列紊乱、染色不均；当关节面压力逐渐减小后，软骨表面逐渐修复，细胞仅在表层排列紊乱③免疫组织化学观察可见整个观察期内软骨细胞胞浆内均有Ⅱ型胶原表达，术后3周内表达逐渐变弱，从第7周时开始逐渐变强。④电镜下可见当关节面压力增大后，软骨细胞逐渐变扁，胞质中内质网膜、高尔基体减少，胶原排列紊乱；当关节面压力减小，软骨细胞形态逐渐恢复正常，胞质中内质网膜、高尔基体数量逐渐恢复；胶原纤维排列逐渐有规则。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。