组织化学的

Immunocytochemistry method was used to demonstrate the expression of glial fibrillary acidic protein.

用塑料吸头划刮培养皿，制造机械损伤的模型，继续培养至72 h，分别取出不同时间点的细胞进行胶质细胞增生情况的观察和免疫组织化学染色，对胶质原纤维酸性蛋白表达产物光密度进行图像分析。

The Sprague-Dawley rats were intratracheally instilled with lipopolysaccharide(LPS,2 mg·kg-1 per day) for two days to induce acute lung injury.The rats were sacrificed at 72 hours after LPS instillation.Lung morphology was studied.

气管内注入LPS复制大鼠急性肺损伤模型，观察72 h后的病理变化、支气管肺泡灌洗液中细胞总数的变化、免疫组织化学特点以及蓝桉油的影响。

Methods: Thirty adult male Wistar rats were randomly divided into three groups: the operation group, the transplantation of MSCs group and the injection of saline group. Functional outcome measurements were performed based on the modified neurological severity score at day 1, 7, 14, 21 and 28 after MACO. The survival, migration, expression of Nestin, glial fibriliary acidic protein and neuronspecific enolase of 5bromo2deoxyuridinelabeled MSCs were detected by immunohistochemical double staining.

线栓法建立左侧大鼠MCAO模型，随机分为3组：手术组、MSCs 移植组、生理盐水组。5溴2脱氧尿核苷标记的MSCs移植后，采用改良神经功能损伤评分系统评价大鼠神经功能恢复情况；应用免疫组织化学双染技术检测MSCs的存活、迁移及其巢蛋白、胶质纤维酸性蛋白和神经元特异性烯醇化酶的表达。

At 7 and 14 days following transection of masseteric nerve through which Fluorogold was applied to identify the Me5 neurons innervating masseter, brain sections were immunohistochemically processed to detect the three Trk isoforms in FG-labeled Me5 neurons.

通过大鼠咬肌神经给予荧光金，标记支配咬肌的Me5神经元；分别于切断咬肌神经后7和14 d对脑切片进行免疫组织化学染色并观察荧光金标记的Me5神经元表达的三种Trk受体。

To observe the influence of tumor necrosis factora on differentiation of rat mesencephalic neural stem cells,the numbers of neurons,astrocytes and oligodendrocytes generated from NSCs were analyzed after differentiation for 3 days by using i m munocytoche mistry technique.

为观察肿瘤坏死因子对神经干细胞分化的影响，本研究应用体外扩增的新生大鼠中脑NSCs，使用免疫组织化学技术，观察了肿瘤坏死因子一。对NSC、分化及其后代细胞的影响。

Methods: Based on the theory of "kidney produces sui", we set up MCAO rat model. Five indexes including Nestin, PSA-NCAM, MAP-2, GAP-43 and SYN38 were measured in situ hybridization and immunohistochemical methods to evaluate the effects of Fujian Tablet on the proliferation, migration, differentiation of neural stem cells, axonal growth and synapse formation. Other two indexes including trk-B, NOGO-A were used to assess the effects on the neurotropic factor and inhibitory factor.

以&肾生髓&的理论为依据，以MCAO大鼠为实验对象，利用原位杂交技术和免疫组织化学方法，分别以nestin、PSA-NCAM、MAP-2、GAP-43、突触素p38为指标，观察复健片对神经干细胞增殖、迁移、分化、轴突生长、突触形成的影响；分别以trk-B、NOGO-A为指标，观察复健片对神经生长营养因子、抑制因子的影响。

Methods 20 cases of brain tissues of intractable epilepsy and 15 cases of nonepileptic brain tissues were collected.

按随机化原则，在我们建立的难治性癫痫患者术后脑组织库中抽取15例患者的颞叶脑组织，用免疫组织化学、免疫荧光法分别检测其NCAM-140kDa的表达，并与对照组进行比较。

After the type 1 diabetic encephalopathy rat models were set by streptozotocin for 5 days, insulin and saline were used respectively to treat the related model rats for 55 consecutive days. Morris water maze and bromodeoxyuridine immunohistochemistry were used to detect the changes in spatial memory and learning, and the neural stem cell proliferation in the subgranular zone of hippocampal dentate gyrus of each rat group.

在用链脲佐菌素建立1型糖尿病脑病模型后的第5天，分别用胰岛素和生理盐水替代治疗55天，再通过Morris水迷宫测试及5-溴脱氧尿嘧啶（BrdU，神经干细胞DNA合成的标记物）免疫组织化学方法，分别观察和比较各组大鼠SGZ神经干细胞增殖和空间学习记忆的动态变化。

The method combined fluorogold retrograde with fluorescent immunohisto chemistry was used to study c-fos expression of the neurons of brian stem proje cting to the subnucleus reticularis dorsalis under noxious stimulation.

用荧光金逆行追踪和FOS荧光免疫组织化学染色相结合的方法对外周伤害性刺激下大鼠脑干内向延髓网状背侧亚核传入投射的神经元中c-fos的表达进行了研究。

Methods Rats aging 4 weeks were immobilized by limb-tail fixation for 2 and 4 weeks respectively, and then the wet weight, gray weight, bone mineral density of femurs and tibiae were measured, the histopathological and histomorphological parameters also were detected, which were used to evaluate the effect of immobilization on bone content and bone structure of femurs and tibiae in growing rats. The VDR expression of femurs and tibiae in the growing rats was evaluated by immunohistochemistry and image analysis.

通过腿－尾固定法对4周龄幼鼠分别进行右后肢2周和4周制动，采用股骨湿重、灰重、骨密度测量，骨组织病理学和骨组织形态学参数测定等方法对比评价制动对幼鼠制动侧股骨和胫骨的骨量、骨结构的影响，采用免疫组织化学和图象分析方法检测制动对幼鼠骨VDR表达的影响。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。