组织化学的

Methods The expression of HIF-1α was assayed by means of immunohistochemical technique in 60 patients.

方法通过免疫组织化学方法，了解HIF-1α在60例原发性颊黏膜鳞癌中的表达，分析其阳性表达水平与颊癌临床分期、病理分级以及预后等临床病理因素之间的关系。

Methods The heat shock protein 70 (HSP70) of rabbit skin was measured by the immunohistochemcial method and the differences of the HSP70 expression in rabbit governor vessel and its bilateral sides skin were compared at 4h and 16h after depilating treatment.

免疫组织化学方法检测热休克蛋白70(HSP70)，比较经脱毛膏脱毛处理后恢复期4h、16h皮肤HSP70表达的差异以及督脉线及其两侧旁开对照点HSP70表达的差异。

The expression of△Np63 mRNA and protein in the endometria of mice were detected by RT-PCR and immunohistochemical technique respectively.

运用RT-PCR技术检测ΔNp63 mRNA的表达规律；免疫组织化学SP法检测小鼠子宫内膜中ΔNp63蛋白的表达情况。

At the end of 2 months,6 months,9 months,12 months,respectively,left eyeballs of the mice for each group were enucleated to make tissue sections for light study and immunohistochemistry analysis.

取2 月龄、6 月龄、9 月龄、12 月龄C57BL/KsJ db/db糖尿病小鼠的眼球，制成石蜡切片，行免疫组织化学染色，进行免疫定位及用计算机图像分析仪对视网膜神经节细胞层的AngⅡ进行灰度和面密度分析。

Results show that only by selecting proper surfactant to disperse the nano-Al_2O_3 powders completely before adding in the plating bath can a fine composite coating with tiny Al_2O_3 well-distributed be obtained.

将纳米Al2O3应用于化学复合镀中，研究了表面活性剂对纳米Al2O3粉的分散状态和-Al2O3纳米微粒复合镀层组织形貌的影响。

Bcl|2 and bax were studied by strept avidin|biontin complex immunohistochemistry.results the expression rate of telomerase in breast cancer was significantly higher than that in precancerous breast(p＜0.01) and in breast benign tumors.

用原位杂交方法检测端粒酶的表达及其活性，采用免疫组织化学技术sabc法检测bcl|2和bax。结果端粒酶在乳腺癌中的表达率明显高于癌前病变（p.01）和乳腺良性肿瘤。

Obtain of transgenic sub-generation seedlings: Altergether there were 892 seeds of 52 transgenic seedlings.Partial seeds bourgeoned and transgenic sub-generation seedlings were obtained.

4转基因株系子一代的获得：获得52个转基因株系的种子总共892粒，将部分种子萌发，培育成小苗，经组织化学染色和PCR鉴定，证实外源基因已遗传至子一代。

Methods Establishing a model of whole cerebral ischemia-reperfusion in rats; examining the expressions of TrkA and NGF on cochleas assuming the method of immunohistochemistry stain; and examining the time and distribution of the cochlear hair cell apoptosis through the terminal deoxynucleotidyl transferasemediated dUTP nick end labeling.

建立大鼠全脑缺血再灌注模型，采用免疫组织化学染色方法检测TrkA和NGF在耳蜗中的表达；用TUNEL法检测耳蜗毛细胞发生凋亡的时相和分布。

The percentage of ERa was approximately 24% in the anterior pituitary gland of adult chicken, and there was no significant difference between laying hens and adult cockerels.

免疫组织化学双标记的结果证明，ERα在成年鸡垂体中所表达的细胞类型主要是LH细胞，而产蛋母鸡中双标记细胞显著高于成年公鸡。

At the end of the culture, PBMCs cultured on coverslips were fixed and stained for tartrate resistant acid phosphatase, a marker of osteoclast.

对培养终末细胞作组织化学染色，检测破骨细胞特征性标志物抗酒石酸磷酸酶的表达；并以象牙磨片上虫蚀样骨吸收陷窝的形成为指标检测其生物学活性。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。