组织化学的

And Sirius Red in a supersaturated picric acid solution viewed by polarization microscopy. The changes of mRNA expression of collagen type I were analyzed by RT-PCR. The results were compared with those after intervention with Losartan.

染色及苦味酸天狼猩红胶原特异染色偏振光显像进行心脏间质胶原的组织化学观察，RT-PCR观察心脏I型胶原mRNA表达的变化，并与氯沙坦干预后比较。

At the end of 12 and 18 weeks, rats were killed, taken the muscle of posterior limb, after separating the gastroenemius、soleus、plantaris, to prepare the frozen section of the each kind of muscle respectively to be confirmed to form the myopathy model by the histochemical stain.(3) RT-PCR、Western blot and immunohisto-chemistry stain were respectively used to detect the expression of NF-κB、MMP-2 and MMP-9 during different period.

2实验组用酒精给大鼠灌胃，对照组用含与酒精体积相等的生理盐水灌胃，两组均喂饲改良的全营养高脂饲料，于12周及18周后，取后肢肌肉，在分离出腓肠肌、比目鱼肌、跖肌后，将各类肌肉均分别地制备成冰冻切片和石蜡切片，经组织化学染色确认成模。

The expression of SRp38 in mouse retina was investigated by Western blot and immunohistochemistry analyses. The result shows that the expression of SRp38 proteins in mouse retina is region-specific, with extensive distribution in the outer and inner plexiform layers, inner nuclear layer and ganglion cell layer, but no expression in outer nuclear layer.

利用Western blot 以及免疫组织化学方法研究了SRp38蛋白在小鼠视网膜中的表达以及分布情况，结果显示，SRp38蛋白在视网膜中的表达具有区域特异性，在外网层、内核层、内网层以及节细胞层中均有表达，而在外核层无表达。

HE's photomicrographs were analyzed for morphology. The proteinic expression of cyclophilin A was observed by immunochemistry. The mRNA's amount of ICAM-1 and P-selectin were quantitated by real-time quantitative reverse-transcript PCRs. All replicate data are expressed as mean±SEM.

每组随机取4例标本通过HE染色切片进行血管形态学分析，并使用免疫组织化学染色方法分析亲环素A在蛋白质水平的表达情况；另每组随机取7只小鼠通过实时定量反转录方法检测ICAM-1与P-选择素mRNA的表达。

The HPV DNA related sequences, the expression of HPV capside antigen and relationship between the ultrastructural feature of lesion and diverse types of HPV infection in 35 cases of the cervical pr61iferative lesions(cervical condyloma, cervical cancer)were analyzed on the levels of cell,subcell and molecule by using nucleic acid hybridization, immunohistochemical method and electron microscopic technique.

本研究分别用核酸杂交、免疫组织化学和电子显微镜术等方法从细胞、亚细胞和分子水平上比较分析了35例子宫颈增殖性病变中人乳头瘤病毒DNA相关序列，人乳头瘤病毒衣壳抗原的表达以及病变超微结构变化特征与不同型别人乳头瘤病毒感染之间的关系。

The gray value of SP and CGRP radioactive particles in bilateral spinal trigeminal caudal subnucleus in rats were detected by immunohisto- chemisrty while obvious damages in trigeminal neurocytes on the injected side were confirmed.

在确认实验侧三叉神经节细胞出现明显破坏的同时，采用免疫组织化学技术，检测大鼠双侧三叉神经脊束核尾侧亚核内P物质和降钙素基因相关肽免疫阳性颗粒的灰度值。

The dynamic distribution of three different glycoconjugates in oocytes and follicle cells during the oogenesis of Atractomorpha sinensis were detected using biotin-labeled Peanut Agglutinin, Soy Bean Agglutinin and Ulex Europaeus Agglutinin I lectins.

以生物素标记的凝集素(UEA-I、 SBA、 PNA)为探针，利用凝集素组织化学方法对短额负蝗卵子发生过程中滤泡细胞和卵母细胞内糖复合物的分布进行了定位研究。

Sections from frontal lobe, occipital lobe, striatum and hippocampus of normal subjects and sections from hippocampus of AD patients were used in hematoxylin eosin, Lox Fast blue, toluidine blue stains and ulex europaeus agglutinin immunostaining.

应用荆豆凝集素(ulex europaeus agglutinin， UEA)免疫组织化学及甲苯胺蓝等染色观察正常增龄病例额叶、枕叶、壳核、海马及AD患者海马的神经元、毛细血管形态改变，应用图像分析技术测算各部位神经元与毛细血管密度比值并分析其与年龄之间的相关性。

Before the two-hour interaction of male-male individuals , we found there were obviously fewer and lighter stained ERp immunoreactive neurons (ER|3-IRs) in both main olfactory bulb system project regions and vomeronasal system project regions in the mandarin voles than in the reed voles, but no significant difference in numbers of AR immunoreactive neurons between the two species through the examining of immunoreactive neurons, moreover, there were significantly fewer AR-IRs than ERp-IRs in two species of vole before the two-hour interactions.

免疫组织化学检测，没有社会交往时棕色田鼠主嗅球系统投射区和犁鼻系统投射区ERβ免疫阳性细胞明显少于沼泽田鼠，且显色淡，AR免疫阳性细胞在两种鼠间差异不大，且都明显少于各自的ERβ-IRs.2h交往后，棕色田鼠主嗅球投射区和犁鼻系统投射区的ERβ-IRs细胞数明显少于交往前，AR-IRs细胞数明显多于交往前；沼泽田鼠交往前与交往后ERβ-IRs和AR-IRs细胞数均无显著差异，且显著多于交往后棕色田鼠ERβ-IRs细胞数，显著少于交往后棕色田鼠AR-IRs细胞数。

METHODS: Fresh human amnions were clipped to pieces which were sutured in a shape like muff by utilizing epidural tubes as a stent. When 4 cm section of ureter in length was excised to replace the defected ureter. 36 rabbit models were divided medially into the 1-month therapy and 3-month therapy groups. At the end of study, all amnion-ureter were sliced and examined with pathologic method or immunohistochemistry to check expressions of α-actin in smooth muscle cells and CKAE1/AE3 in transitional epithelial cells.

新鲜人羊膜裁剪成片状，以硬膜外导管为支架，做成袖套状，替代4 cm的大白兔输尿管缺损。36只成模大白兔均分为1个月治疗组和3个月治疗组，实验结束时，取羊膜输尿管行病理学检查和免疫组织化学检查平滑肌细胞α-Actin和移行上皮细胞CKAE1/AE3的表达情况。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。