组织化学的

Methods Both in malformed embryos induced by excess retinoid acid and normal KM mice, expressions of lectin receptors of WGA, RCA and ECL in the epithelium of palatal shelves and the mesenchyma were determined by affinity histochemistry using biotin-marked corresponding lectins.

以生物素标记的凝集素受体WGA、RCA和ECL为探针，应用亲和细胞组织化学染色方法检测上述3种凝集素受体在RA致畸鼠胚和相应对照组鼠胚腭板上皮及其间充质的分布状况和变化规律。

Methods The expression of bcl-2 and bax were examined by immunohistochemical ABC staining with specific antibodies in 34 cases of uterine leiomyosarcomas (18 cases in stage Ⅰ, 1 in stage Ⅱ, 8 in stage Ⅲ, and 7 in stage Ⅳ), 34 uterine leiomyomas and 34 normal myometrium samples.

用免疫组织化学ABC法检测34份子宫平滑肌肉瘤标本（Ⅰ期18例，Ⅱ期1例，Ⅲ期8例，Ⅳ期7例）和34份子宫平滑肌瘤以及34份正常子宫肌层标本中的bcl-2及bax蛋白的表达。

With the HRP orthograde tracing technique and transmitted electron microscope,the effect of CNG on the optic nerve was observed by the changes of axonal transport and ultrastructure of optic nerve.

用辣根过氧化物酶(horserdish peroxidase，HRP)组织化学染色法和透射电镜技术观察CNG对急性高眼压视神经顺行轴浆运输和超微结构的影响，定量分析CNG的药物疗效。

The positve rates in serous carcinomas and borderline tumors were 39.3% and 55.6%, significantly lower than that in benign tumors and normal ovaries (P.05), but the statistical differences were not found between borderline tumors and serous carcinomas, benign tumors and normal ovaries.2 WWOX mRNA and protein expression in ovarin seruous tumors2.1 WWOX mRNA expression in ovarin seruous tumors The result of RT-PCR showed that WWOX mRNA expression were detected in 29 of 32 ovarian serous benign tumors, 12 of 16 ovarian serous borderline tumors, and 21 of 45 ovarian serous carcinomas respectively.

阳性条带密度扫描定量结果，卵巢浆液性癌中PTEN蛋白表达相对量为0.34±0.12比正常卵巢和浆液性囊腺瘤和交界性囊腺瘤明显降低(p.05)，其余各组间无统计学差异。3.2.2 PTEN蛋白FCM检测结果FCM结果，卵巢浆液性癌PTEN蛋白表达的FI值(0.908±0.023)明显低于正常卵巢、卵巢囊腺瘤和交界性囊腺瘤的FI值，差异有统计学意义(P.05)。3.2.3 PTEN蛋白免疫组织化学结果35例浆液性囊腺瘤、18例交界性囊腺瘤和56例浆液性癌中，分别有31例、15例和29例呈阳性表达。

Immunohistochemical staining with an anti-type II collagen antibody demonstrated rich production of the type II collagen in the pericellular matrix from the chondrocytes.

用抗II型胶原抗体免疫组织化学染色证明在软骨细胞周围的基质中有丰富的II型胶原产生。

Histological changes of periostea on the mandibular ramus after the periosteal distraction:2.1 20 adult New Zealand rabbits were operated and distracted with the same methods mentioned above.2.2 Every 5 animals were killed postoperatively at days 28,35,42 and 56 respectively.

2.2、术后第28、35、42和56天分别处死5只实验兔，每组随机选其中2只取实验侧和对照侧的下颌升支骨膜进行HE染色观察；每组3只实验兔取实验侧和对照侧的下颌升支骨膜进行增殖细胞核抗原免疫组织化学染色观察。

MSCs are a homogenous population of cells that have unique growth, phenotypical and cytochemical characteristics.

MSCs是一群均一的细胞，具有独特的增殖、表征及组织化学特征。

Methods The positive stained area was compared between 22 STC cases and 12 non obstructed colon cancer cases using immunohistochemistry technique and pathology micrograph analysis system with 100 multiple photics microscope.

方法应用免疫组织化学技术和病理显微镜分析图像系统，在中倍光镜下计算22例STC患者和12例非梗阻性结直肠癌患者的阳性染色区域的面积并进行对比。

Method; A rat model of acute cerebral ischemia was built by photochemically initiated thrombosis . Vegf - mrna was observed with RT - PCR. Vegf and cell proliferation were observed with immunohischemistry.

用光化学法制作局部脑皮质梗死模型，针刺治疗3天后，应用RT—PCR检测梗死边缘区 VEGF mRNA的表达，用免疫组织化学方法检测VEGF表达和细胞增殖，用激光多谱勒检测造模后 2 h和72 h的血流情况量。

Methods 181 cases of mammary gland pryma who had accept the examination of the nearinfrared TBOI dualwave length mammary gland phyma dedecfor were classified by nearinfrared parameters of blood oxygen,and were performed the pathologic exmination to ascertain whether tumor is benign or malignant.

用近红外光TBO－I型双波长乳腺肿瘤检测仪，对检测的181例乳腺肿块进行近红外光血氧参数分类和病理切片检查明确肿块性质，并对其中20例恶性肿块和20例良性肿块进行免疫组织化学SP法检测乳腺肿块微血管密度，运用医学统计学方法分析近红外光血氧参数与微血管密度的相关性。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。