组织化学的

The immunohistochemical location of AChE was often used to study the nervous system of Platyhelminthes, but distribution of AChE in Hydra pseudoligactis remains poorly understood.

乙酰胆碱脂酶组织化学定位方法常用于研究动物的神经系统，但迄今来见水螅AChE分布定位的相关报道。

In order to investigate the effect of IFN-γon the expression of TNF-αin the hypothala- mus-pituitary-ovary axisof RU486-induced aborted SD rats, RU486 was used to establish the abortion model, then the aborted rats were administrated with different doses of IFN-γintraperitoneal injection, the method of immunohistochemical ultrasensitive SP was us- ed to study the variation of positive TNF-αcells in hypothalamus, pituitary, ovary and uterus in normal pregnant group A, aborted model group B and three different doses of IFN-γgroup C,D,E. The main goal of this research was to study whether the low dose of IFN-γcould have a protective function to pregnancy and the mechanism of its function so as to provide morph- ologic basis for the scientific using of IFN-γin the process of animal abortion control.

为探讨IFN-γ对流产大鼠下丘脑-垂体-卵巢轴(hypothalamus-pituitary- ovary-axis，HPOA )TNF-α表达的影响，本试验用米非司酮(RU486)建立SD大鼠流产模型，通过腹腔注射不同剂量IFN-γ，采用超敏感免疫组织化学SP法(链霉素抗生物素—过氧化物酶法)，观察了孕13天(d13)SD大鼠正常妊娠A组、流产模型B组、不同剂量IFN-γC、D、E组下丘脑、垂体、卵巢、子宫TNF-α的表达变化，探讨低剂量IFN-γ对妊娠是否有保护作用及其作用机制，期望能为在妊娠过程中如何科学应用IFN-γ防止动物流产提供形态学依据。

The aim of this study was to investigate PGT, 15-PGDH and CBR1 expression in themouse uterus during early pregnancy and their regulation in related models of pseudopregnancy,delayed implantation, artificial decidualization, steroid hormone treatment by in situ hybridization,RT-PCR and immunohistochemistry.

本研究利用原位杂交、免疫组织化学和RT-PCR等方法，检测了PG转运载体PGT、代谢酶15-PGDH和CBR1在小鼠早期妊娠子宫中的表达，并利用假孕、延迟着床及激活、人工蜕膜化、性腺类固醇激素处理等模型，研究了它们在小鼠子宫中的表达与调节。

In addition,the expression of Galβ-1,4-GlcNAc group was studied by ricinus communis agglutinin-I lectin blot and lectin histochemistry with RCA-I respectively.

并利用特异识别-β1，4-半乳糖苷键的蓖麻凝集素-Ⅰ，经letin blot和组织化学方法分析-β1，4-半乳糖苷键在各型人脑星形胶质细胞瘤中的表达变化和表达定位。

Methods :The Alcianblue Safranin and immunohistochemical staining were employed to observe the density of MC, PCNA and oncogene bcl-2 expression in the specimens of 74 cases with gastric cancer.

采用阿尔辛蓝-沙红及免疫组织化学染色法，同步检测74例胃癌标本中的肥大细胞数及PCNA、bcl-2基因蛋白的表达。

The rabbit model of osteoarthritis was established according to Hulth's method. The rabbits were sacrificed at the 8th week after administration of Yanghe Decoction for 14 days, and then rabbit tibia articular cartilage was removed. Sections of the cartilage were stained with Safranin O for histological examination. The cartilage histological characteristics were observed according to the method of Mankin. Immunohistochemical staining was performed to investigate the expression of VEGF.

按照Hulth法建立膝骨关节炎模型，术后第5周起至第7周末，阳和汤组予混有阳和汤的颗粒饲料，正常组及模型组均给予普通饲料；术后第8周分别取各组胫骨平台关节软骨，采用番红O染色，进行Mankin评分；免疫组织化学染色测定关节软骨中VEGF表达的阳性指数。

Concentrations of DA and 5-HT in different regions were measured by high performance liquid chromatography; the expression of SERTmRNA was detected by in situ hybridization; the degeneration of neuron and terminal was demonstrated by Bielschowsky and Glee Marsland silver staining; and the protein expression of GFAP was detected by immunohistochemistry.

2采用高效液相色谱法测定不同脑区神经递质DA和5-HT的含量；采用原位杂交方法检测SERTmRNA；Bielschowsky及Glee Marsland氏改良银浸染法显示神经元胞体、树突和轴突；免疫组织化学方法检测GFAP的表达。

Cerebral hemorrhage rat models were established via injection of autologous arterial blood in caudate nucleus. Two days after modeling, 5 μL BrdU-labeled human embryonic NSCs suspension was transplanted at four points surrounding hematoma cavity in the rats. After 1 and 2 weeks, rats were sacrificed. Adjacent sections were doubly stained by BrdU/microtubule-associated protein 2 (MAP-2) and BrdU/glial fibrillary acidic protein.

通过注射自体动脉血到尾状核制作大鼠脑出血模型，出血后2 d将标有5'-溴脱氧尿嘧啶的人胚神经干细胞悬液移植到血肿腔周围的4点，1，2周后处死大鼠，相邻脑组织切片行5'-溴脱氧尿嘧啶/微管相关蛋白2和5'-溴脱氧尿嘧啶/胶质纤维酸性蛋白免疫组织化学双染。

METHODS: Cerebral cortex cells of 8-week aborted human fetus were harvested and cultured in vitro to obtain human embryonic NSCs. Cerebral hemorrhage rat models were established via injection of autologous arterial blood in caudate nucleus. Two days after modeling, 5 μL BrdU-labeled human embryonic NSCs suspension was transplanted at four points surrounding hematoma cavity in the rats.

通过注射自体动脉血到尾状核制作大鼠脑出血模型，出血后2 d将标有5'-溴脱氧尿嘧啶的人胚神经干细胞悬液移植到血肿腔周围的4 点，1，2周后处死大鼠，相邻脑组织切片行5'-溴脱氧尿嘧啶／微管相关蛋白2和5'-溴脱氧尿嘧啶／胶质纤维酸性蛋白免疫组织化学双染。

METHODS Using immunohistochemistry method, we observed the Fos expression in the brain of purified protein derivative of tuberculin immunized mice at different time points.

用免疫组织化学方法观察纯化的结核菌素衍生蛋白免疫刺激后不同时间点小鼠脑内的 Fos蛋白表达。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。