组织化学的

Fig.1 SHEE cultured on coverslide, the living cells were growing in single layer with rich cytoplasm, the nuclei were uniform in size with a nucleolus ph ×400 Fig.2 SHEE had a nucleus with ellipse shape, large nucleolus and the cytoplasm contained mitochondria and tonofibrilEM ×10 000 Fig.3 SHEE was spherical in shape, with pseudopods attached on petri dish and abundant villi on cell surface SEM ×5 000 Fig.4 Same as in Fig.3, cell attached on petri dish, appeared stellate or polygonal in shape, with abundant pseudopods and cytoplasmic processes. Protrusive nuclear region in central part of the cell had more micro-villi SEM ×5 000 Fig.5 Chromosomes of SHEE cells belonged to diploidy type Giemsa ×1 000 Fig.6 The SHEE cells of stained in dark brown by Ki67 immunohistochemistry were the proliferative cells Immunohistochemistry ×400 Fig.7 In SHEE cell culture, the nucleus stained red or pink by PI was dead cell, the green nucleus was living cell Fluorescent ×400 Fig.8 The cell labeled by TdT was apoptotic cell in which the chromatin of nucleus condensed in block, a pyknotic nucleus in the upper right conner was seen TdT labeled ×400

图1 SHEE培养在盖坡片上，活细胞单层生长，胞浆较丰富，细胞核大小一致，有核仁×400 图2 SHEE培养细胞细胞核椭圆形，核仁较大，胞浆有较丰富的线粒体和张力原纤维EM ×10 000 图3 SHEE细胞呈球状，有伪足贴壁，表面有密集微绒毛SEM ×5 000 图4 同上细胞贴壁，呈星状或多角形，有丰富伪足和胞浆突，核区隆起有较多微绒毛SEM ×5 000 图5 SHEE细胞染色体仍属二倍体Giemsa染色×1 000 图6 SHEE细胞Ki67免疫组织化学染棕黄色为增殖细胞×400 图7 SHEE培养细胞出现死细胞，胞核和胞浆PI染色呈红色或淡红色，蓝色细胞核为活细胞荧光显微镜×400 图8 细胞TdT标记阳性为凋亡细胞，染色质凝集呈块状，右上角有一固缩细胞核TdT标记×400

Immunohistolochemical stain by monoclonal antibodies for growth factors and actin was used to detect whether there are abnormal expressions in saccular aneurysms.

利用血小板源性生长因子、转化生长因子（TGF-β1）、血管内皮细胞生长因子和肌动蛋白的单克隆抗体进行免疫组织化学染色。

This experiment which adopting technology of examination of muscle, utilizing modern organized chemical method, using scanning photometer, doing a eight-week staminal training towards six ordinary examinee, analyses the samples of the vastus lateralis of the right leg before and after the experiment.

采用肌肉活检技术，运用现代组织化学方法，结合扫描显微分光光度计定量的方法，对6名普通受试者进行8周耐力跑训练。

METHODS: The neonatal and adult rats facial nerve was ligated at stylomastoid foramen.

于新生及成年大鼠茎乳孔处结扎面神经，于3， 7， 14， 21， 28， 56， 84 d采用NADPHd组织化学方法观察NADPHd阳性细胞在面神经核中的变化。

To observation the change of thyrohyoid muscleafter recurrent laryngeal nervehas been cutting off.

通过建立RLN麻痹动物模型，应用组织化学染色及显微图像分析，动态地观察THM三型肌纤维直径和面积的变化。

Then glutamate, GABA and GABA_BR1 in cells on bone trabecula surface of distal fermur of rats in every group were checked with immunohistochemistry method.

运用免疫组织化学方法检测各组大鼠远端股骨骨小梁表面细胞的Glu、GABA和GABA_BR1。

Methods Expression of MMP-8, collagen Ⅰ and collagen Ⅲ in fascia transversalis from the patiens of indirect inguinal hernias and non hernias was examined by immunohistochemical method.

采用免疫组织化学方法检测30例腹股沟斜疝患者及25例非病患者腹横筋膜中MMP-8、胶原Ⅰ、胶原Ⅲ的表达。

Histoehemistry method was used to study the distribution of ulex europaeus agglutinin-l（UEA-1） receptors in rabbit uterus during the estrus cycle and early pregnancy.

采用组织化学方法对荆豆凝集素-1（ulexeuropaeusagglutinin-1，UEA-1）受体在兔发情周期及早期妊娠子宫内的分布以及激素调节进行了研究。

Histochemistry method was used to study the distribution of ulex europaeus agglutinin-1(UEA-1) receptors in canine uterus during the estrus cyctle and early pregnancy.

采用组织化学方法对荆豆凝集素-1(ulex europaeus agglutinin-1，UEA-1)受体在发情周期和早期妊娠犬子宫内的分布以及激素调节进行了研究。

The expression of AT1 and AT2 receptor was examined in ureteric bud cell of the embryonic mouse, including embryonic days 12, 14, 15, 16 by immunohistochemical technique.

采用免疫组织化学方法检测胚龄12，14，15，16 d小鼠肾脏发育早期输尿管芽分支中血管紧张素Ⅱ受体1和受体2的含量。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。