组织化学的

Methods Expression of livin protein were detected by immunohistochemistry staining assay in 45 osteosarcoma cases and 30 osteochondroma cases.

采用免疫组织化学SABC法检测45例骨肉瘤、30例骨软骨瘤凋亡抑制蛋白Livin表达，并分析其与临床病理因素的关系。

Methods Pancreatic cancer cell panc-1 was treated with oxamate for 48 h,the mitochondrial membrane potential was measured by flow cytometry(Rodamin-123),and the activity of intracellular lactate dehydrogenase was studied by enzymohistochemistry.

方法体外培养人胰腺癌细胞panc-1，分别加入不同浓度的草氨酸盐培养48 h后，行酶组织化学染色及罗丹明123染色，观察细胞糖酵解关键酶乳酸脱氢酶活性及线粒体膜电位。

On the basis of light microscopic observation the immunohistochemical examination of CK,TG,NSE,vimentin and calcitonin was used to observe 9 cases of oxyphilic cell adenoma of the thyroid.

观察9例甲状腺嗜酸细胞腺瘤，在光镜观察的基础上，用免疫组织化学方法检测细胞角蛋白、甲状腺球蛋白、神经元特异性烯醇化酶、波形蛋白和降钙素。

Peribulbar injection of dexamethasone 0.5mg was applied in the experimental group every other day 48 hours after operation to the end of the experiment, and the growth state of conjunctiva in both groups were observed. 2 HA implants from experimental group and control group respectively were extracted for testing the positive expression of VEGF by immunohistochemistry 3,7,14,21,28 days after operation.

术后滴妥布霉素滴眼液4次/d，实验组术后48h起隔日球周注射地塞米松0.5mg和实验结束，并观察两组的结膜生长情况；实验组与对照组分别于术后 3，7，14，21，28d摘取2只HA植入物行免疫组织化学检测VEGF阳性表达情况。

To evaluate the role of collagen Ⅰ,Ⅲ and Ⅳ in the formation of the pericystic layer of hepatic hydatid cyst The expression of collagen Ⅰ,Ⅲ and Ⅳ in pericystic layers of hepatic hydatid cysts from 40 patients was observed using immunohistochemical methods.

探讨Ⅰ、Ⅲ和Ⅳ型胶原在形成肝包虫囊肿周围人体纤维囊壁中的作用及其临床意义。采用免疫组织化学方法检测Ⅰ、Ⅲ和Ⅳ型胶原在40例肝包虫囊肿周围纤维囊壁中表达。Ⅰ、Ⅲ和Ⅳ型胶原在肝包虫囊肿周围纤维囊壁中出现特异性分层表达。

Open-field test,step-down avoidance test and immunohistochemstry staining were employedto study the effects of the extract of the fruit of Hyperieum perforatum on praxiology and cerebral pathology in the rat models.

制作大鼠慢性应激抑郁模型，应用敞箱法、跳台法、免疫组织化学染色研究贯叶连翘提取物对抑郁大鼠行为学、脑病理学的影响。

He volume of cerebral infarction was calculated by TTC staining, the expressions of NF-κB、Bcl-2 and Bax proteins in procerebrum cortices and basal nuclei of rats were detected by immunohistochemistry.

TC染色法测量脑梗死体积，免疫组织化学方法检测前脑皮质、基底核NF-κB、Bcl-2和Bax蛋白的表达。

METHODS: Pterygial specimens harvested from clinical operations received morphology, immunohistochemistry and immunofluorescence under a confocal microscope.

对手术切除的翼状胬肉标本进行形态学、免疫组织化学和免疫荧光共聚焦显微镜观察。

Methods Immunohistochemistry method was used to stain the substance P in pulpal tissue which could directly reflect the activation of nerve by stimuli.

应用免疫组织化学染色的方法，对树脂充填深龋洞后不同时期P物质表达变化进行观察研究。

The immunohistochemical method was used to detect Fos expression in Central Nerve System induced by pulpitis pain.

应用免疫组织化学方法检测牙髓炎疼痛诱发中枢神经系统（central nerve system， CNS）c-fos的表达。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。