组织化学的

With the methods of molecular biology, in situ hybridization, immunohistochemistry, immunohistochemistry, radioimmunodetection, Electrophysiology, we have studied the depressant effect for hypermyotonia of experimental cerebral infarction rat and its the gamma-aminobutyric acid receptor mechanisms first time when we simulated the GB 34 with electric acupuncture.

本课题以大鼠实验性脑梗死动物模型为研究对象，采用分子生物学原位杂交技术、免疫组织化学染色法、放射免疫检测技术、神经电生理记录方法，首次较系统研究了电针阳陵泉对脑卒中动物肢体肌张力增高的抑制作用及其GABA受体机制。

The expression of Galectin-3 and S100A4 in 68 cases of thyroid follicular hyperplasic nodule was detected by immunohistochemistrical Envision method beside 17 cases of follicular adenoma, 6 cases of adenomatous nodular goiter, 14 cases of follicular variant of papillary carcinoma and 31 cases of follicular carcinoma.

采用免疫组织化学方法检测68例甲状腺滤泡性增生结节中Galectin-3和S100A4蛋白的表达，包括17例滤泡性腺瘤，6例腺瘤性甲状腺肿，14例滤泡亚型乳头状癌和31例滤泡癌。

Immunofluorescence staining and western blotting indicated that hyphal fragments also enhanced the expression of TLR2 and TLR4 in protein level.

荧光免疫组织化学和western blot结果显示灭活菌丝片断能促进TLR2和TLR4蛋白水平的表达，而且这种表达能进一步被氢化可的松上调。

The development and metastasis process of orthotopic tumor models was observed directly by fluorescence microscope,and the size of the hypodermal tumor was measured by vernier.

眼内肿瘤生长情况用荧光显微镜直接观察，皮下肿瘤大小用游标卡尺测量；采用免疫组织化学方法对肿瘤内13种基因的表达进行检测。

MAIN OUTCOME MEASURES: Lacunae changes of osteocytes in femoral heads were detected with hematoxylin-eosin staining method, and the expression of Bcl-2 and Bax proteins with immunohistochemistry staining methods, after 3, 6, 12, 24, 48 and 96 hours of ischemia respectively.

主要观察指标：应用苏木精-伊红染色，免疫组织化学来观测股骨头缺血后3，6，12，24，48，96h股骨头内骨细胞陷窝变化及Bcl-2、Bax蛋白表达的变化情况。

Methods Specific endothelial cell markers with immunohistochemistry and microvessel density with morphometry in 28 casas of laryngopharyngeal malignant melanoma.

方法应用免疫组织化学染色法检测28例咽喉部恶性黑色素瘤中血管内皮细胞特异性标记物Ⅷ因子相关抗原（factor FⅧ-related antigen， FⅧ-RAg）和CD31的表达，并通过形态学计量法计数肿瘤内微血管密度。

By using the immunohistochemistry method of Strept Avidin-Biotin-Complex, four kinds of antisera raised against rabbit hormones: somatostatin, pancreatic polypeptide, 5-hydroxytryptamine （ 5-HT ） and neuropeptide Y were used to indentify and locate the endocrine cells in the digestive tract of Sparus latus.

应用4种兔抗胃肠激素抗体和SABC免疫组织化学方法，对黄鳍鲷消化道中的内分泌细胞进行鉴别和定位。

Methods In both malformed embryos induced by excess retinoid acid and normal KM mice,expressions of Dishevelled2 and Vangl2 in the epithelium of the palatal shelves and the mesenchyma were determined by immunohistochemistry and image analysis.

方法采用免疫组织化学及图像分析方法，检测维甲酸致畸小鼠和相应对照组小鼠腭板中Dishevelled2和Vangl2蛋白的表达状况。

In the past, the origin of these tumors was thought to be mesothelial cells.

在过去认为这种肿瘤是由肋膜生长出，但藉由免疫组织化学染色技术的进步，我们了解事实上孤立性肋膜纤维瘤是由肋膜下肺间叶所生长出之肿瘤。

METHODS: Thirty male SD rats were randomly divided into control group, stress group and NG-nitro-L-arginine methyl ester group. The rat model of water immersion-restraint stress was established. The expression of nNOS in colonic submucous plexus and myenteric plexus in the rats was examined by immuno-histochemical staining and analyzed by computer image analysis system.

SD大鼠30只随机分为对照组，应激组和L-NAME组，采用水浸-束缚应激动物模型，用免疫组织化学ABC法检测nNOS在大鼠结肠黏膜下神经丛和肌间神经丛的表达，应用计算机图像分析系统对其表达进行定量分析。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。