组织化学的

Methods: 30 adul male Sprague-Dawley rats were divided randomly into model group and control group. The rats were injected into lateral ventricle with coriaria lactone (50g/kg). The sections of the brains were processed by immunochemistry with antibodies against SYN and GFAP.

成年健康雄性SD大鼠30只，随机分为模型组及对照组，采用马桑内酯(50g/kg)侧脑室注射法建立急性癫痫动物模型，应用免疫组织化学方法观察癫痫持续状态下SYN和GFAP的表达。

The model mice were then treated by giving GDNF into the striatum. After a seven-day survival period, the model mice were sacrificed to get the segment of substantial nigra fixed, embedded and coronally sectioned continuously. The microsections were processed by immunohistochemistry using anti -TH and anti-CB antibodies to label DA neurons and CB-containing neurons respectively, which were counted under microscope and analyzed statistically.

利用1-甲基－4-苯基1，2，3，6-四氢吡啶(1-methyl-4-phenyl-1，2，3，6-tetrahydropyridine，MPTP)制备成年小鼠PD模型，通过单侧纹状体定位注射GDNF，取中脑黑质节段，做连续冠状石蜡切片，结合免疫组织化学染色方法，观察各组酪氨酸羟化酶(tyrosine hydroxylase，TH)、钙结合蛋白(calbindin D28k，CB)的表达，光镜观察并细胞计数，统计学分析。

The mechanism of Si Junzi Tang and panax sapogenol were studied on the effect of gastrointestinal cells protection by applying advanced technology of histochemistry, Holt′s cytodieresis mircocirculation, radio-immunity and subcellular ultrastructure.

进而应用酶组织化学、Holt细胞分裂测定、微循环测定、放射免疫测定、亚细胞超微结构观察等先进技术，研究四君子汤、人参皂甙抗胃肠细胞损伤、保护胃肠细胞的作用机理。

Mehtods Thirty male SpragueDawley rats were randomly divided into acute stress,chronic stress and control group(each n=10),acute and chronic stress models were made with forced swimming and empty bottle stimulation separately,and frequency of getting into central grille, detention time , outer grille numbers,modifying numbers and dejecture grain number were observed with openfield experimental box;expressions of neuropeptide Y and adenylcyclase in prefrontal lobe cortex, hippocampus CA3,striatum of the groups were observed using the immunohistochemistry method and correlation analyses conducted.

将30只雄性SD大鼠随机分为急性应激组、慢性应激组和正常对照组，每组10只，用强迫游泳和空瓶刺激分别制作急性和慢性应激模型，采用开野实验箱观察各组大鼠进入中央格次数、停留时间、外周格子数、修饰次数、粪便粒数；采用免疫组织化学方法检测各组大鼠神经肽Y、腺苷酸环化酶在前额叶皮质，海马CA3区，纹状体的表达水平，并进行相关分析。

Mehtods Thirty male Sprague??Dawley rats were randomly divided into acute stress,chronic stress and control group(each n=10),acute and chronic stress models were made with forced swimming and empty bottle stimulation separately,and frequency of getting into central grille, detention time , outer grille numbers,modifying numbers and dejecture grain number were observed with open??field experimental box;expressions of neuropeptide Y and adenylcyclase in prefrontal lobe cortex, hippocampus CA3,striatum of the groups were observed using the immunohistochemistry method and correlation analyses conducted.

将30只雄性SD大鼠随机分为急性应激组、慢性应激组和正常对照组，每组10只，用强迫游泳和空瓶刺激分别制作急性和慢性应激模型，采用开野实验箱观察各组大鼠进入中央格次数、停留时间、外周格子数、修饰次数、粪便粒数；采用免疫组织化学方法检测各组大鼠神经肽Y、腺苷酸环化酶在前额叶皮质，海马CA3区，纹状体的表达水平，并进行相关分析。

Methods The NGF, BDNF, and NT3 genes of rats were cloned, the eukaryote expression vectors were established, the three kind of recombinant vectors were used to transfect astrocytes, the positive cloned cells were cultured dilatedly after G418 sifting; using supernates of culture liquid of astrocytes modified by gene to culture PC12 or TrkB-PC12, the expression and its level of gene target cells aimed genes were measured by Western blotting or immunohistochemical method.

克隆大鼠NGF、BDNF和NT3基因，构建真核表达载体；3种重组载体分别转染星形胶质细胞，G418筛选后获得阳性克隆细胞进行扩大培养；取基因修饰星形胶质细胞培养液上清培养PC12细胞或TrkB-PC12细胞；用Western blotting杂交或免疫组织化学方法检测基因靶细胞目的基因的表达及其水平。

METHODS:The distribution changes of N CAM at flatfish muscle neuromuscular junction of rat in its embryon phase,14 day after birth,adulthood and 10,20 and 30 days after its L5 lumber nerve root injury were observed by immunohistochemistry and confocal laser scanning microscopy technologies.

采用免疫组织化学方法和激光共聚焦显微扫描技术，观察了大鼠胚胎期，出生后14d，成年期以及L5神经根受压后10，20，和30d，大鼠比目鱼肌神经肌肉接头部N-CAM的分布变化。

Expression of CD44s and fibronectin were evaluated by immunohistochemistry method in entopic from 22 patients and in ectopic from 45 patients with endometriosis and the endometrial tissues of 20 non-endometriosis .

方法采用光镜和SP 免疫组化法法检测子宫内膜异位症在位内膜(22例)、异位内膜(45 例)、对照组子宫内膜(20 例)进行病理组织化学观察和CD44s、FN 表达的检测。

Rats in the normal group did not receive any intervention.③Proliferating cell nuclear antigen,β-Ⅲ tubulin and glial fibrillary acidic protein expression in ependyma was determined by the immunohistochemical staining and image analysis.

实验评估：采用免疫组织化学染色法和图像分析技术检测增殖细胞核抗原、β－Ⅲ型微管蛋白（Tuj-1）、胶质原纤维酸性蛋白在室管膜下的表达。

The cells from hippocampus, stria-tum, ependyma, thalamus, olfactory bulb, cortex, brain stem and cerebellum of the brain were studied using HE staining and im-munocytochemistry.

应用苏木精-伊红染色和免疫组织化学方法观察人胎脑室管膜下区、海马、纹状体、丘脑、嗅球、皮质、脑干及小脑的Nestin阳性细胞。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。