组织化学的

Antibody of mouse against rat of glial fibrilla acidic protein used to labeling astrocytes of the brain and antibody of rabbit against rat of c-fos labeling c-fos protein in the brain. The second fluorescence antibodies were FITC and Cy3, respectively. Observations of the results and quantitative analysis were conducted with LSCM.

在本实验中，我们应用免疫组织化学的双标方法，用小鼠抗大鼠胶质原纤维酸性蛋白标记大鼠脑内的星形胶质细胞，用兔抗大鼠c-fos标记大鼠脑内的c-fos蛋白，分别应用FITC和Cy3标记的荧光二抗来显示，最后用激光扫描共聚焦显微镜进行观察并定量分析。

With the development of physiology,immunohistochemistry and application of electron microscope,researchers want to know the ultramicrostructure,3D ultramicrostructure and physiologic function of ICCs,to think highly of the effect of ICC,and try to interpret morphosis and physiologic function about it.

随着电镜的应用及生理学、免疫组织化学的发展，人们对Cajal间质细胞的超微结构、三维网络结构及生理功能有了进一步的认识，开始重视Cajal间质细胞的作用，试图阐明它们的形态结构和生理功能。

1.To study the expression of in the different glucose concentration with the time changing. 2.To study the effect of different concentration of Hepatocyte Growth Factor on the Integrin-linked kinase in protein and mRNA level by Mesangial Cells in high concentration (25 mmol/L) of glucose. 3.To study the effect of different concentration of HGF on the Fibronectin'secretary by Mesangial Cells. 4.To study latent therapeutic action and possible mechanism of HGF on the glomeruler sclerosis. The difference of ILK's experession on mRNA level in different concentration of glucose and HGF are detected by means of revers transcription-polymerase chain reaction,and by means of immunohistochemistry to detect their difference in protein level;the effection of HGF to the FN's secretary in high glucose are detected by means of enzyme linked innunosorbent assay.

1探讨在不同的血糖浓度下大鼠肾小球系膜细胞中ILK伴随时间变化在蛋白和mRNA水平表达的差异 2探讨不同浓度的HGF对高糖环境下(25mmol/L)大鼠肾小球系膜细胞中ILK在蛋白和mRNA水平表达的影响 3探讨不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响 4初步探讨HGF对肾小球硬化的潜在的治疗作用及其可能机制采用RT-PCR的方法分析在不同血糖浓度和不同HGF浓度下大鼠肾小球系膜细胞中ILK在mRNA水平表达的差异；采用免疫组织化学的方法直观的观察它们在蛋白水平表达的差异；采用ELISA法分析不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响。

In the present study changes of NOS positive cells after injection of LPS and apoptotic ependymal cells, the relationship between expression of immediate early gene c-fos and astrocytes, and comparison of changes between SFO and rest regions of the brain in different time-points were investigated with NADPH-d histochemistry, apoptosis in SITU detection of TUNEL, TEM and SEM, fluorescein double labeling of immunohistochemistry, and laser scanning confocal microscope .

为了证实穹窿下器在脑内免疫调节系统中的作用，在本研究当中，应用了NADPH-d的组化方法、TUNEL末端标记法、扫描及透射电镜方法、免疫组织化学的荧光双标法及激光扫描共聚焦显微镜技术，来研究在腹腔内给予大肠杆菌内毒素后，脑内的一氧化氮合酶细胞的变化、室管膜细胞的凋亡、早期即刻基因c-fos的表达与星形胶质细胞之间的关系，以及穹窿下器与其它脑区相比较时的特异性改变。

In the present study changes of NOS positive cells after injection of LPS and apoptotic ependymal cells, the relationship between expression of immediate early gene c-fos and astrocytes, and comparison of changes between SFO and rest regions of the brain in different time-points were investigated with NADPH-d histochemistry, apoptosis in SITU detection of TUNEL, TEM and SEM, fluorescein double labeling of immunohistochemistry, and laser scanning confocal microscope.

为了证实穹窿下器在脑内免疫调节系统中的作用，在本研究当中，应用了NADPH刁的组化方法、TUNEL末端标记法、扫描及透射电镜方法、免疫组织化学的荧光双标法及激光扫描共聚焦显微镜技术，来研究在腹腔内给予大肠杆菌内毒素后，脑内的一氧化氮合酶细胞的变化、室管膜细胞的凋亡、早期即刻基因（immediate early genes沁－fos的表达与星形胶质细胞之间的关系，以及穹窿下器与其它脑区相比较时的特异性改变。

Methods: The expression level of NOS and NGF was detected in pterygopalatine ganglion of the allergic rhinitis rats using histochemieal and immunofluorescence staining methods respectively.

建立AR大鼠模型，用HE染色方法观察鼻黏膜内腺体的变化；用组织化学的方法观察翼腭神经节内NOS表达的变化；用免疫荧光的方法观察翼腭神经节内NGF表达的变化。

In control mice, mild intensity of immunoreactive staining of phosphorylated ERK1/2 was seen in the neurons of the marginal zone and the substantia gelatinosa in the caudal subnucleus of the trigeminal spinal nucleus (Sp5C).

免疫组织化学的观测表明，正常小鼠延髓尾侧亚核Ⅰ和Ⅱ层的神经元存在微弱水平的ERK1/2活性，左右侧无明显区别；尾侧亚核神经元存在高水平的磷酸化CREB。

The FosB/△FosB positive staining was detected in nucleus accumbens septum, tuberculum olfactorium and dorsal caudate putamen, without little cFos positive staining. The results indicate the expression of cFos and FosB/△FosB display the differences in area and intensity, and the area intensively expressing FosB/△FosB is more nearly coincide with the area related to schizophrenia. So FosB/△FosB is a better mark to research the pathogenesis of schizophrenia.

同时大量研究证明，动物模型的行为及生理变化与中枢神经系统神经元的病理变化有关，因此，利用即早基因反应的灵敏性、功能多样性、检测方便性等，应用免疫组织化学的方法对模型小鼠脑内cFos和FosB/△FosB蛋白的表达进行检测，通过确定不同脑区cFos和FosB/△FosB蛋白的表达，来研究哪些脑区或神经元参与了反应，从而发现精神分裂症在脑区内所涉及的解剖结构，有助于对精神分裂症发病机制的认识。

This change in morphology is associated with important physical and cytochemical changes and with an increase in their capacity for motility and their fertilizing ability.

这种形态学的变化是与重要的物理学和组织化学的变化以及活力和受精能力的增强有关。

LDH in culture fluid, SOD, XOD, free calcium in L-6TG cell and mitochchondral respiration were evaluated in each group; apoptosis was detected by flow cytometer with PI staining method and agarose gel electrophoresis; the immuneohistochemical method was used to determine the expression of bcl-2 and Caspase-3 ; the morphoiogic changes were observed with microscope.

将培养的L-6TG大鼠肌母细胞随机分为3组：①正常对照组，②缺血再灌注组（1R组），③缺血预处理组，观测了培养上清中LDH、细胞内SOD、XOD、Ca(上标 2+)含量的变化；采用MTT法检测线粒体的功能；利用流式细胞仪和细胞DNA电泳结果检测细胞凋亡情况；采用免疫组织化学的方法检测bcl-2及Caspase-3的蛋白表达情况，结合自动图像分析系统对其结果进行定量分析；在光镜下观察细胞的形态学改变。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失