组织化学的

METHODS Expression of Bcl-2,Bax and P53in endothelial cells of cremaster ischemia-reperfusion of the rat was investi-gated by means of immunohistochemical SP method.

以免疫组织化学SP法对大白鼠提睾肌缺血再灌注后血管内皮细胞Bcl-2，Bax及p53的表达进行观察。

The nucleus of the transfected cells were crenation or broken down into pieces, which proved that ERIP1 could induce apoptosis.

在免疫组织化学实验中，将带有GFP标签的ERIP1转染入HEK-293T和MCF-7细胞，发现细胞大面积死亡，细胞中转染入质粒后，细胞核皱缩、破碎，说明ERIP1可能诱导细胞凋亡。

Methods:ABC methods of immunohistochemistry and image analysis technique were used to study the changes of 5-HT in nucleus raphe dorsalis after cerebral ischemia and reperfusion in different time.

用免疫组织化学ABC法及图象分析对沙土鼠脑缺血不同时间以及缺血10分钟后再灌不同时间其中缝背核神经元5-HT的变化进行研究。

The results of Western blotting and immunohistochemical method showed that HO-1 protein expression increased in DOX group and DOX -IR group,and was negative in normal group.

Western blotting法和免疫组织化学S-P法检测显示DOX 组和DOX-IR组 HO-1蛋白表达增加，HO-1在正常肝脏内的表达呈阴性。

Results: The tHey and blood fat level in blood plasma and expression of MCP-1mRNAof aorta on model group is obviously higher than that of control group (P＜0.05);the result of immunohistochemistry shows that the expression of VCAM-1 and MMP-2enhanced, Bcl-2 is lower; aorta slice shows endarterium discontinuation, strippingpartly, smooth muscle cell arrange puffly and chaotic, infiltration of lipoid ofdifferent degree are observed in aorta wall, pathological changes suffusion, morexanthoma cell, plaque protrude lumens. The they and blood fat level in blood plasmaand expression of MCP-lmRNA, VCAM-1, MMP-2 of aorta on intervention group aremuch lower than that of model group (P＜0.05); Bcl-2 of intervention group isstrengthened, and the pathomorphology and immunohistochemistry of aorta showpathological changes are slighter than model group.

结果：模型组，血浆中同型半胱氨酸浓度、血脂水平、主动脉MCP-1mRNA表达显著高于正常对照组(P＜0.05)，免疫组化示主动脉内皮细胞VCAM-1、MMP-2表达增强，Bcl-2表达降低；主动脉切片发现动脉内膜不连续，部分剥脱，平滑肌细胞排列疏松、紊乱，主动脉壁均见到程度不同的脂质浸润，其病变弥漫，泡沫细胞较多，可见斑块突出管腔；干预组，血浆中同型半胱氨酸含量、血脂水平显著低于对照组和模型组(P＜0.05)，主动脉MCP-1mRNA、VCAM-1、MMP-2表达显著弱于对照组和模型组(P＜0.05)，Bcl-2表达增强(P＜0.05)，主动脉病理形态学和免疫组织化学检测示病变轻于模型组。

By action on HNF3β,HNF6 play some role in endoblast differentiation.Reverse transcription PCR can detect HNF6 gene expression in all tissues. Western blot can detect HNF6 protein expression in all tissues.

本研究结合免疫组织化学、逆转录PCR和Western杂交技术，分别在基因和蛋白质水平上初步探索了转录因子FOXF1、Notch2和HNF6在人类肝癌中的表达规律。

Using immunohistochemical and eosin stain, expression of ERK1/2 and Elk in the lung of asthma model was observed.

三、应用免疫组织化学和伊红复染方法，观察了在体哮喘模型肺内ERK1/2和Elk的表达。

In vivo: to examine oncogenicity by heterotransplantation into nude mice in situ and pathological characteristics with HE and immunohistochemistry staining.

2体内实验研究：裸鼠原位接种检测该细胞株的致瘤性，组织化学染色观察其病理学特性。

Results were investigated by macroscopical impression, weight, thickness, immunohistochemistry after explantation at 4 and 8 weeks.

有新生血管形成，免疫组织化学检测证实脂肪细胞的存在及良好分布。

Methods Immunostaining for expression vascular endothelial growth factor was carried out in 40 cases of transitional cell bladder carcinoma.

应用LSAB免疫组织化学技术检测40例膀胱移行细胞癌血管内皮生长因子的表达。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。