组织化学的

The acidophilic granules in the proximal convoluted tubule of the kidney in Tilapia nilotica were studied by electron microscopy, enzyme histochemistry and enzyme cytochemistry.

用电镜和酶组织化学技术探明了尼罗罗非鱼肾近曲小管细胞嗜酸性颗粒的结构实质、来源和存在意义。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Using CP (Colophony-Paraffin) embedding tissue section technique and immunocytochemical method (Streptavidin-Peroxidase, SP), we have investigated the distribution of GABA and 5-HT immunoreactive neurons in the brain of female and male ants, Camponotus Japonicus. Special attention has been paid to the comparative research on the differences between female and male ants. We also try to give possible interpretations leading to these differences in the behavioral aspects.Here we show that in the brain of female and male C.

本实验采用树脂石蜡组织包埋切片技术(国家发明专利ZL98125709.7)和链霉菌抗生物素蛋白-过氧化物酶(Streptavidin-Peroxidase，SP)免疫组织化学方法，对日本弓背蚁雄蚁和雌蚁成虫脑中的GABA和5-HT能神经元的分布进行了详细的研究，比较了雄蚁和雌蚁的异同，并对可能造成这些差异的行为学联系进行了初步的探讨。

The dynamic changes of the number of T or B lymphocytes and the content of immunoglobulin G, IgM, IgA in peripheral blood, the number of CD〓, CD〓 lymphocyte subpopulation, T〓 cells and IgG, IgM, IgA antibody-producing cells, the ratios of CD〓 and CD〓 T cells in immune organs (thymus, bursa of Fabricius and spleen) and immune tissues (cecal tonsils and Harderian glands), the content of IgA, IgM, IgG in local fluid (tear, tracheal fluid, intestinal fluid and bile) of one-day chicks infected with chicken infectious anemia virus and CIAV-infected chicks immunized with Newcastle disease vaccine were determined respectively by immunohistochemistry, immunogold-silves and immunoenzyme staining in order to reveal mechanism of immunopathogenesis of chicks infected with CIAV and influence of immune responses of CIAV-infected chicks to ND vaccine.

中文题名雏鸡感染鸡传染性贫血病毒后免疫变化和对新城疫疫苗的应答及其免疫机理副题名外文题名 Immune changes of chicks infected with chicken infectious anemia virus and immune response and mechanism to Newcastle disease vaccine immunity 论文作者郑世民导师刘忠贵教授学科专业基础兽医学研究领域\研究方向学位级别博士学位授予单位东北农业大学学位授予日期2002 论文页码总数105页关键词鸡传染性贫血病雏鸡新城疫疫苗馆藏号BSLW /2003 /S858 /9 本项研究采用免疫组织化学、免疫胶体金及免疫酶方法，首次对CIAV感染1日龄雏鸡和CIAV感染雏鸡ND疫苗免疫及vNDV攻击后，其外周血液的T细胞、B细胞数量和IgG、IgM、IgA含量；中枢免疫器官胸腺和法氏囊，外周免疫器官脾脏及呼吸道和消化道局部粘膜免疫组织哈德尔腺和盲肠扁桃体的ANAE〓、CD4〓、CD〓T细胞、IgG、IgM、IgA抗体生成细胞数量和CD〓与CD〓T细胞比值以及泪液、气管液、肠液、胆汁的IgA、IgM、IgG含量的动态变化规律和CIAV感染对ND疫苗的免疫保护率等进行了全面系统地研究。

The expression of Bcl-2 and Bax and Bak protein were examined with immunohistochemical method in the experimentation, the relationship between the protein expression and the clinical features of laryngeal carcinoma, as well as the relationship among them were discussed. We could probe into the safe incisal range of removing laryngeal cancer determined from the albumen balance of cells to conduct our clinical work.

本实验旨在通过免疫组织化学方法，检测Bcl-2、Bax和Bak蛋白在喉鳞状细胞癌组织中的表达情况，探讨它们与喉癌的临床指标的关系，探讨三者之间的关系，并从蛋白质水平探讨喉癌手术的安全切缘的范围为临床治疗提供参考。

The findings of the newly identified goose paramyxovirus strain GPMV/HBexperimental infection were as follows: The challenge caused high morbidity and highmortality.The clinical signs included severe depression, decreased appetite or anorexia,white,yellow and green diarrhea, with neural symptoms;pathological changes showed thathaemorrhage and ulceration of intestine mucosa, necrosis of spleen and pancreas,andedema of liver and kidney, uratic deposition in kidney.Eosinophilic inclusion bodies werefound in the cytoplasm of pancreas and a large quantities of fiber protein were found inintestine. Lots of hemosidefin was found in some area of liver and kidney. A number ofT、B lymphocytes reduced sharply in spleen,lymph nodes in dead geese. Transmissionalelectron microscope showed that some cells had the morphological characteristics ofmitochondria swelling, morphological changes were condensation of nuclear chromatininto dense masses,followed by its marginating against the nuclear envelope, andespecially concentration of cytoplasm association with lots of lipid droplet.

人工感染雏鹅实验中，雏鹅临床表现为高发病率和高死亡率，食欲和饮水减少，拉白色、黄绿色稀粪，伴有神经症状；常规组织学观察，脾脏和胰腺肿大，有白色坏死斑点，肠道出血和坏死，肝脏肿大变性，肾脏肿大变性有尿酸盐沉着等；用特殊染色和组织化学方法首次对人工感染鹅副粘病毒的雏鹅的重要脏器进行研究，结果表明：胰腺中发现病毒包涵体，肠道中大量纤维素以及胶原纤维增生，肾脏、肺脏大量含铁血黄素沉着，免疫器官如脾脏和淋巴结中T、B淋巴细胞大量减少；电镜下多种细胞呈现线粒体水肿，多种实质细胞的染色质固缩，染色质边集，特别是细胞内容物如脂肪滴增多的病理变化特征。

BE1 monoclonal antibody can be used to detect basophils in tissue.

BB1单克隆抗体可有效地用于支气管肺组织标本的嗜碱性粒细胞免疫组织化学检测，同时急性发作的致死性哮喘患者气道中嗜碱性粒细胞明显增加，可能与哮喘病情恶化、甚至死亡有关，而非致死性的慢性哮喘患者气道中嗜碱性粒细胞与对照组无异，说明嗜碱性粒细胞可能主要参与哮喘的急性病程。

Detecting each marker and cmparison the location of sequential tissuesection could determine the area loceation relationship of the markers, but it is difficulte to determine their correlation intra-cells exactly. No report of detecting virus genome and antigenes on a one section with triple staning techniques exists in the lit erature. in this report. by combining immunohistochemistry and in situ hybridization (with dioxigenin labelled HBV DNA probe), a triple staining technigce was established. With this technic, on one formalin-fixed and paraffin embeded section, HBV DNA, HBsAg and HBcAg were determinated one after the other.

各个细胞内病毒的复制和表达水平以及与肝细胞病变的关系，是乙型肝炎发病机理研究的一个重要方面：目前常用的在系列切片上对各标志物分别检测，比较观察的方法，一般只能大致了解各待检物在某一区域的定位关系，很难准确地了解它们在同一细胞内的关系：国内外尚未见有在同一张切片上三重标记染色检测病毒核酸及其多种抗原的报道：本文结合免疫组织化学ABC法及地高辛配基标记HBV DNA探针原位杂交，建立三重标记染色技术：在一张福尔马林固定石蜡包埋肝组织切片上先后检测，同时显示HBV DNA、HBcAg和HBsAg。

11 guinea pigs which were successfully induced myopias were extracted eyeballs, and the scleras near posterior poles were observed by immunohistochemistry methods. The positive rates of TGF-β〓 and bFGF in scleral fibroblast cytoplasma and the area percentageratio of positive cells were calculated and contrasted to controlled eyes.

将透镜诱导近视成功的11只豚鼠处死，摘除眼球，取后极部巩膜分别进行TGF-β〓和bFGF的免疫组织化学染色，观察它们在巩膜组织成纤维细胞胞浆中中表达的阳性率和阳性细胞所占面积的百分比，并与对照眼进行比较。

11 guinea pigs which were successfully induced myopias were extracted eyeballs, and the scleras near posterior poles were observed by immunohistochemistry methods. The positive rates of MMP-2 and TIMP-2 in scleral fibroblast cytoplasma and the area percentageratio of positive cells were calculated and contrasted to controlled eyes.

将透镜诱导近视成功的11只豚鼠处死，摘除眼球，取后极部巩膜分别进行MMP-2和TIMP-2的免疫组织化学染色，观察它们在巩膜组织成纤维细胞胞浆中表达的阳性率和阳性细胞所占面积的百分比，并与对照眼进行比较。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。