组织化学的

Our results demonstrate: The structures of the organs are normal, and the shapes of cells are clearly visible. There are lots of positive brown granules in Chief cells and Parietal cells in abomasum as well as the mucosa epithelial cells and gland cells of duodenum. Three bands with a molecular mass close to 120KDa、110KD and 98KDa were identified by Western Blot. The Ob-R levels of 120KDa in abomasum were significantly higher than that of in small intestine. The levels of 110KDa were similar in the two organs. The expression of 98KDa Ob-R was weak.

HE染色结果显示，各组织结构正常，细胞形态清晰可见；免疫组织化学SABC染色显示，在皱胃胃体部固有层胃底腺的主细胞和壁细胞及十二指肠黏膜上皮细胞和固有层肠腺的柱状细胞中均可见大小数量不等的棕黄色颗粒；western Blot 实验发现，在胃和小肠均检测到120KDa、110KDa和98KDa三条带。120KDa长型瘦素受体蛋白在胃中表达量显著高于小肠中的表达；110KDa的短型瘦素受体蛋白，在小肠和皱胃中表达量接近。98KDa短型受体蛋白在胃和小肠表达均较弱。

The changes of MDA in the plasma, CINC/IL-8 and NO in the serum, MPO in the skeletal muscle were studied at the end of ischemia and 1h, 2h, 4h, 8h, 12h during reperfusion.

分别于缺血4h末，再灌注1、2、4、8、12h，测定各组血浆中的MDA、骨骼肌组织中的MPO，血清嗜中性粒细胞化学趋化因子(CINC／IL-8)，血清一氧化氮，用流式细胞仪及免疫组化法观察各组白细胞上粘附分子CD11b／CD18和骨骼肌血管中ICAM-1的表达情况及各组的组织学改变。

This study was conducted to determine the expression of the basic fibroblast growth factor in degenerated intervertebral discs.

本实验的目的是应用免疫组织化学和原位杂交的方法检测bFGF在退变的椎间盘组织中的表达。

Methods Immunohistochemical method with two specific antibodies(MMP-9,CD68) was used to detect the their special antigens in lung tissues of six cases who died of SARS and five normal cases as the controls.Gomori aldehyde fuchsine staining method was used to explore the change of elastic fiber.Motic advanced 4.0 image analysis system was used to analyze those images.

方法通过免疫组织化学技术对6例SARS死亡患者肺和5例肺外伤患者手术切除的正常肺组织中MMP-9和CD68+细胞进行检测，Gomori醛品红法显示弹性纤维，应用Motic Advanced 4.0图像分析系统进行定量分析，测定MMP-9的面密度、CD68+细胞的数量、弹性纤维的含量，并与正常对照组进行比较。

Methods:Thirty six mice were inoculated with the sarcocarcinoma 180 according to the standard method,after 10 days,weighed the tumor and body.The expressions of bcl 2 and p53 proteins in the tissue were studied by immunohistochemistry; and the activities of SOD, GSH Px and the content of MDA were detected by colorimetric method in serum.

按照标准方法给36只小鼠接种S180肉瘤后，给予珍珠梅提取物10 d，观察瘤重及体重，免疫组织化学方法检测肿瘤组织中的bcl 2和p53蛋白，比色分析法检测血清SOD，GSH Px的活性及MDA含量的变化。

Methods TGFβ1 protein in 53 cases of RA synovia ［including 26 cases of RA,20 cases of control non-RA (14 cases of OA and 6 cases of traumatic arthritis) and 7 cases of normal synovia］ was detected by immunohistochemical LSAB method.

采用免疫组织化学LSAB法检测了53例(其中26例RA，20例其他关节炎和7例正常人)滑膜组织中TGFβ1蛋白的表达情况，并用目镜网格测微尺和图像分析系统分别进行TGFβ1阳性细胞的半定量计数和TGFβ1染色强度及面积的定量分析。

On the sixtieth postoperative day, the graft was harvested and the blood was taken from inferior cava venae. Histological changes were monitored by HE stain. The infiltration of CD4+ and CD8+ T lymphocytes and the expression ofα- smooth muscle actin were detected by immunohistochemistry.

术后60天取移植血管做病理组织学检查并利用计算机图象分析系统测量血管内膜厚度；免疫组织化学检测CD4+、CD8+T淋巴细胞的浸润及α-平滑肌肌动蛋白的表达；ELISA法检测血清中血管内皮生长因子的变化。

Immunohistochemistry were positive for CD34 in some region where the fibroblast actively proliferated, whereas fibrocytes in mature fibrous tissue were negatively stained.

CD34免疫组织化学染色显示一些增生活跃的纤维母细胞明显表达CD34，成熟的纤维组织内的纤维细胞CD34则为阴性。

Methods The MCs and TAMs count were determined immunohistochemically in 60 patients with colorectal cancer , and investigate the relationship of the number of MCs and TAMs with depth of invasion、lymph node metastasis rate、 distant metastasis rates and survival rates.

采用免疫组织化学方法对60例大肠癌根治术手术标本的MC及TAM进行检测，并探讨MC亚型和TAM与大肠癌的组织学类型、浸润深度、淋巴结转移及生存率之间的关系。

Methods After injecting different doses of cephalosporins(100, 200, 300, 400μg) into vitreous body of rabbits, we studied the active change of retinal enzymes at different time and observed the histological and ultrastuctural changes of retinae.

方法以不同浓度的先锋霉素Ⅵ（100，200，250，300，400μg）行兔眼玻璃体腔内注射，用酶组织化学染色法测定注射后不同时间（1，3，7d）视网膜琥珀酸脱氢酶和乳酸脱氢酶活性的变化，并观察对视网膜组织结构及超微结构的影响。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。