组织化学的

Histochemical analysis showed that the specific expression of GUS in seeds was not restricted to the endosperm cells, but also occurred in the vascular bundle and epithelial cells of scutellum.

组织化学分析发现在水稻种子专一性表现的GUS活性并不局限於胚乳细胞，维管束组织及子叶盘的上皮细胞也有明显的表现。

Ojective To investigate the distribution of H type 1-4 chains of ABO histo-blood group in adult human kidney and their relationships with secretor status.

应用抗ABO抗体及3种糖链特异的单克隆抗H抗体的免疫组织化学方法，检查分泌型与非分泌型个体肾组织中相应抗原物质的分布。

Expression of MMP-2 and TIMP-2 was examined by immunohistochemical technique and scored semiquantitatively(score 0-3) in carcinoma cells, and the results were correlated with clinicopathological tumor parameters.

我们通过免疫组织化学染色观察人胰腺癌组织MMP-2和TIMP-2的表达情况及其与侵袭转移的关系，旨在探讨MMP-2和TIMP-2表达对胰腺癌患者预后的影响。

MethodsExpressions of SP and vasoactive intestinal peptide in tongue base tissues of 49 patients with OSAHS were analyzed by immunohistochemical staining.

方法通过免疫组织化学染色对49例OSAHS患者的舌根组织的P物质及血管活性肠肽的表达进行分析。

METHODS: expression of Pgp and MDR1 mRNA were detected by immunohistochemistry and reverse transcriptionpolymerase chain reaction in the specimens from 25 breast cancer patients with neoadjuvant chemotherapy, 15 breast cancer patients without neoadjuvant chemotherapy and 10 patients with breast adenosis.

采用免疫组织化学及逆转录多聚酶链反应技术，对25例接受术前新辅助化疗的乳腺癌患者、15例未接受术前化疗的乳腺癌患者、10例乳腺腺病患者组织标本内Pgp 和MDR1 mRNA的表达情况进行检测。

METHODS: The expression of Pgp and MDR1 mRNA were detected by immunohistochemistry and reverse transcriptionpolymerase chain reaction in the specimens from 25 breast cancer patients with neoadjuvant chemotherapy, 15 breast cancer patients without neoadjuvant chemotherapy and 10 patients with breast adenosis.

采用免疫组织化学及逆转录多聚酶链反应技术，对25例接受术前新辅助化疗的乳腺癌患者、15例未接受术前化疗的乳腺癌患者、10例乳腺腺病患者组织标本内Pgp 和MDR1 mRNA的表达情况进行检测。

Using Colophony-Paraffin embedding tissue section technique and immunohistochemical streptalidin-peroxidase method, we have investigated the distribution of 5-HT neurons in the brain of Camponotus japonicus, compared the immunoreactivity with GABA, and primarily discussed the character of 5-HT distribution.

本实验采用树脂石蜡组织包埋切片技术和链霉菌抗生物素蛋白-过氧化物酶免疫组织化学方法，研究了5-HT能神经元在日本弓背蚁大工蚁脑中的分布情况，并与GABA的分布进行了比较，初步探讨了5-HT在各个脑区的分布规律和特点。

Recombinatino polypeptide or chemically synthesized polypeptide antigen is used to immunize the mammal to obtain polyclonal antibody and the nonconservative amion acid sequence of the antiboby to N-terminal with recognition site qBrn-2 of development regulatory protein qBrn-2. The antibody does not recognize other POU structure domain protein, and recognizes specifically qunique protein in Western imprinting experiment. The qBrn-2 expression mode obtained immunohistochemistry process is identical with the resulted height of in-situ molecular hydridization.

以重组多肽或化学合成多肽为抗原免疫哺乳动物获得的多克隆抗体，这一抗体对发育调控蛋白qBrn－2的识别位点为qBrn－2的N－末端的非保守性的氨基酸序列，该抗体不识别其它POU结构域蛋白；在Western印记实验中，该抗体特异地识别单一的蛋白；利用免疫组织化学接示的qBrn－2表达模式与分子原位杂交的结果高度一致。

The expression status of 〓 andRB protein in the tissues of normal, chronic pancreatitisand pancreatogenic carcinoma were detected by immunohis-tochmical staining. Also, the relationship be-tween the feature of cancer and prognosis was analysed in combi-nation with clinical data.

采用免疫组织化学染色，检查胰腺癌、慢性胰腺炎、正常胰腺等组织的〓蛋白和RB蛋白表达状态，并结合临床资料，将其与胰腺癌的生物学特性及予后的关系加以分析。

Methods:Thirty six mice were inoculated with the sarcocarcinoma 180 according to the standard method,after 10 days,weighed the tumor and body.The expressions of bcl2 and p53 proteins in the tissue were studied by immunohistochemistry; and the activities of SOD, GSHPx and the content of MDA were detected by colorimetric method in serum.

按照标准方法给36只小鼠接种S180肉瘤后，给予珍珠梅提取物10 d，观察瘤重及体重，免疫组织化学方法检测肿瘤组织中的bcl2和p53蛋白，比色分析法检测血清SOD，GSHPx的活性及MDA含量的变化。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。