组织化学的

Objective To explore a new method of cochlear sectioning to get high qualitative cochlear section catering for immunohistochemistry of cochlea.

目的 探索一种新的耳蜗组织切片方法，以获得高质量的切片，满足其免疫组织化学染色的需要。

RESULTSdiabetic rats showed typical symptoms involving elevated serum glucose and weight loss (2)The rats in DM+VaD group reacted slower and made more error numbers in Y-maze than the rats in VaD group did, with a prolonged total reacting time in a whole day.(3)More neurons with serious damages, such as edema, ischemia or pyknosis could be found in DM+VaD group than in VaD group. More disarrangement of cone neurons, obvious glia proliferation, and more neuron apoptosis were found in CA area of hippocampus. CONCLUSIONS (1)2-VO in STZ-induced diabetic rats succeeded in establishing VaD animal models.(2) The cognitive dysfunction induced by VaD rats had been deteriorated by diabetes mellitus.(3)Morphology evidences proved that diabetes mellitus aggravated brain damages induced by VaD.PART II Effect of diabetes mellitus on the cholinergic nervous systemin CA1 area of hippocampus of VaDOBJECTIVE To estimate the role of cholinergic nervous system in hippocampus during the process of cognitive dysfunction aggravated by diabetes mellitus in DM+VaD group. METHODS Observe the changes of ChAT protein by immunohistochemistry stain. Chose three rats randomly from each group at each pointat two week, four week, and eight week, then sepa

经腹腔注射链脲佐菌素诱导慢性实验性糖尿病，1周后永久性结扎双侧颈总动脉(2-VO)制作VaD模型记录术后2周、4周和8周各组大鼠的体重及血糖；应用Y-迷宫检测空间定向学第二军医大学博士论文中…丈摘…要1ia；一色恤~﹁染澎糕行HE染色观察组织病理学孪叱；胶质细胞原纤维酸性蛋白protellZ，Cf冰尸标记观察星形胶质细舱的激活情况、橄声软公点娜育票票众糯黑橄居端粗默孺篇筑价井袱第二部分糖尿病对血管性痴呆大鼠海马c心区胆碱能神经系统的影响探讨海马CAI区胆碱能神经系统在糖尿病加重确D一大鼠认知障碍中所实验动物及分组同第一部分，应用免疫组织化学染色方法现察海马CAI{介狱牛第晕军l袭拔常博粗{论义

Histochemical, immunochemical and in situ hybridizative methods and technique of computer image analysis were used in this study to investigate the variable trends of expression and correlation of some proteins and genes interrelated with abnormal proliferation of hepatocyte during periods of hepatocarcinogenesis induced by diethylnitrosamine, and the effect of Ganfujian Granule, which is composed of drug-food double-duty herbs, taking"jian-pi-shu-gan"(invagorating spleen and soothing liver) as therapeutic principle and manufactured by our department. The purpose of the study is to screen out an applicable and effective liver cancer preventive agent of traditional Chinese medicine, to offer academic foundation for further clinic study and to spread out its application.

本课题通过组织化学、免疫组化、原位杂交等方法及计算机图像分析等技术，检测了化学致癌物二乙基亚硝胺诱导大鼠肝癌形成过程中与肝细胞异常增殖相关的部分蛋白、基因表达的动态变化和相互关系及我科研制的以"健脾疏肝"立法的药食两用中药制剂肝复健冲剂对诱发肝癌各时期上述因素异常变化的影响，旨在筛选适用、有效的中药肝癌预防制剂，并为进一步的临床试验及推广应用提供一定的理论依据。

Methods Bioptic samples were taken from 8 cases of hypertrophic scar, 8 cases of superficial scar and 16 cases of controlled normal self-skin.MC-1R immunohistochemistry staining and its grey scale were examined in each sample.

利用兔抗人MC-1R抗体进行免疫组织化学染色，并测定8例增生性瘢痕，8例表浅性瘢痕和16例同体的正常皮肤标本切片染色后的平均灰度值，分别检测MC-1R在上述不同组织中表达的差异。

MethodsBioptic samples were taken from 8 cases of hypertrophic scar, 8 cases of superficial scar and 16 cases of controlled normal self-skin.MC-1R immunohistochemistry staining and its grey scale were examined in each sample.

利用兔抗人MC-1R抗体进行免疫组织化学染色，并测定8例增生性瘢痕，8例表浅性瘢痕和16例同体的正常皮肤标本切片染色后的平均灰度值，分别检测MC-1R在上述不同组织中表达的差异。

Immunohistochemical method: The CD34 monoclonal antibody was selected as the first antibody, with the immunohistochemical SP procedure, then the pathological slice was displayed by DAB and stained again by hematein. One slice was used as a negative control and reacted with PBS fluid(0.01mmol/L) which was the substitute of anti- CD34.(the kits coming from Boster Biotechnology CO.LTD). The number of the vessels and vascular endothelial cells were displayed by immunohistochemical method.

免疫组化方法：病理组织切片以CD34单克隆抗体作为第一抗体，按照免疫组织化学SP步骤进行，DAB显色，苏木素复染，每组用0.01mmol/l的PBS液替代CD34抗体作阴性对照(试剂购自于武汉博士德公司)，应用免疫组化显示血管，对不同时期的血管生成情况及早期的炎性反映情况进行观察。

Methods Paraffin imbedding tissues from 60 cases with BC were detected for expression of COX-2 and VEGF by immunohistochemistry,the relationship of its expression with clinical pathological parameters were analyzed.

S-P免疫组织化学方法检测60例乳腺癌石蜡包埋组织中COX-2和VEGF的表达，分析其表达之间的关系及其与临床病理参数的关系。

Methods:MIF、p53 expression and microvessel density from 63 cases of ESCC tissue were researched with immunochemistry technology.

方法应用免疫组织化学技术对63例食管鳞癌组织中的MIF、p53的表达，新生血管热区的CD34阳性微血管密度进行研究。

Methods The expressions of Survivin and VEGF were studied via immunohistochemical method in 60 cases of astrocytoma and 12 normal brain samples. The correlation between these two expressions and their relationship with the pathology were analyzed.

采用免疫组织化学SP法检测60例人脑星形细胞瘤组织和12例正常脑组织中Survivin、VEGF基因的表达，并分析其与临床病理特征的关系以及两者间的相互关系。

MethodsThe expressions of Survivin and VEGF were studied via immunohistochemical method in 60 cases of astrocytoma and 12 normal brain samples. The correlation between these two expressions and their relationship with the pathology were analyzed.

采用免疫组织化学SP法检测60例人脑星形细胞瘤组织和12例正常脑组织中Survivin、VEGF基因的表达，并分析其与临床病理特征的关系以及两者间的相互关系。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。