组织化学的

Methods Five groups (n=24) of SD rats were randomly assigned to received intrapleural injection of dexamethasone, dipopolysaccharide, erythromycin, hypertonic saline and normal saline, respectively. The AQP1 protein in pleural was detected with immunohistochemistry. The mRNA expression of AQP1 under stimulations at different time points was measured by real time RT-PCR.

SD大鼠按实验设计分为5组(n=24)：地塞米松组、内毒素组、红霉素组、高渗盐水组及对照组，通过免疫组织化学染色测定AQP1在胸膜上的表达和定位；采用Real-time RT-PCR方法对不同刺激因子作用下不同时间胸膜组织AQP1的基因表达进行定量分析。

Methods The CXB combining BM was implanted into the sacrospinalis muscle of rabbits, and CXB implanted alone was used as control. Eighteen Japanese rablits with longear were used. The size of CXB was 5 mm×5 mm×5 mm, and the implanted materials were taken out at 2、 4、 8、 12、 16 and 24 weeks after implantation.

将CXB与BM复合及单纯CXB植入兔的骶棘肌内，术后2、4、8、12、16及24周取出植入材料作组织学及组织化学检查，观察植入材料的成骨作用。

The central part was composed of hypertrophic chondrocytes, for which the number increased with time. The cartilaginous tissue at the time of 2 weeks' cultivation appeared strongly positive staining of collagen type Ⅱ immunohistochemistry and safranine "O", as well as strongly metachromatic to toluidine blue.

其中心为肥大软骨细胞，且随培养时间的延长而增多。2周时，软骨组织甲苯胺蓝染色呈强的红色异染反应，Ⅱ型胶原免疫组织化学染色呈强阳性，番红&O&染色呈强阳性。

Methods Immunohistochemical and morphometrical analysis methods were performed on vital skin wounds. Results In normal and injury mouse skin IL-10 immunoreactivity was observed in epidermal cells.

方法应用免疫组织化学技术和形态计量法，对小鼠不同时程皮肤切创组织中IL-10不同表达部位（表皮细胞及表皮下组织的浸润细胞）的变化进行研究。

Objective To investigate clinicopathological characteristics of unicystic ameloblastoma and histological markers for differential diagnosis with odontogenic cyst.

目的 研究单囊型成釉细胞瘤的临床病理及凝集素免疫组织化学特点，探索有助于诊断和鉴别诊断的组织学标记物。

MTA was used to manage clinical problems. These included pulpless permanent teeth with incomplete root formation and perforation in roots or furcations. Total 10 cases (12 teeth), former 7 cases (7 teeth) and latter 4 cases (5 teeth), were present. The follow-up periods were various from 6m to 21m.

应用免疫组织化学手段，观察细胞外非胶原蛋白骨唾蛋白（bonesialoprotein，BSP）、骨桥素（osteopontin，OPN）和骨钙素（osteocalcin，OC）在根尖周组织愈合期间的表达，尤其是在与倒充填材料直接相邻区域的表达。

Methods: The fibroblasts companying human hepatoma were primarily cultured with explant culture method, first passaged after they spread to the bottom of the culture bottle, and pured by enzyme digestion and repeated strapping the cells were identificated through observing the morphologic change using invert microscope and detecting expression of vimentin, keratin by iMmunohistochemical method.

应用组织块培养法进行人肝癌伴生成纤维细胞的原代培养，细胞铺满培养瓶底后首次传代，通过胰酶消化法和反复贴壁法进行成纤维细胞的纯化；通过倒置显微镜观察细胞形态、免疫组织化学方法检测细胞膜波形蛋白、角蛋白表达情况，对细胞进行鉴定。

The results of GUS histochemical assays and PCR amplification demonstrated that the overall transformation rate of Roselle callus was 4%.

GUS活性组织化学检测和PCR扩增鉴定的结果表明，愈伤组织的转化率为4%。

GUS staining shows that GUS gene was expressed in stems, leaves, calyxes, stamens and siliques in transgenic Arabidopsis plants, with higher expression in green tissue such as stems, leaves, siliques, while invisible in roots, petals, and seeds. The data indicate that MPBQ MT gene promoter was likely to be expressed preferentially in green tissues such as stems, leaves, and young siliques.

GUS组织化学染色结果表明，在MPBQ MT启动子驱动下，报告基因GUS在拟南芥的茎、叶、花萼、雄蕊、种荚均有表达，且在茎、叶、种荚中表达量较高，而在根、花瓣和种子中则没有观察到GUS基因的表达，表明MPBQ MT基因可能仅在拟南芥幼嫩茎、叶、种荚等绿色组织中特异性高表达。

Methods: The expression of eIF4E in both 124 cases of endometrioid adenocarcinoma and 20 cases of normal endometria, which was detected by immunohistochemistry, was analyzed in combination with these patients' clinical pathological data.

应用免疫组织化学技术检测124例子宫内膜样腺癌及20例正常子宫内膜组织中eIF4E的表达情况，同时结合患者的临床病理资料进行分析。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。