组织化学的

Methods SP immunohistochemical staining was used to detect the level of VEGF and EG-VEGF in 85 cases of the epithelial ovarian tumor tissue(25 cases of benign tumor,27 cases of ovarian carcinoma of stage FIGOⅠ、Ⅱ,33 cases of ovarian carcinoma of stage FIGOⅢ、Ⅳ)and 20 cases of normal ovarian tissue.The integral optical density was measured.

方法应用免疫组织化学SP法和形态定量学方法检测85例卵巢上皮性肿瘤(良性25例，FIGOⅠ、Ⅱ期卵巢癌27例，FIGOⅢ、Ⅳ期卵巢癌33例)和20例正常卵巢(除外卵巢疾病的绝经前患者)组织中VEGF、EG-VEGF表达的阳性率及积分光密度值。

Methods The expression of Pgp and MT in 121 breast cancer patients was determined by immunohistochemistry.

采用免疫组织化学SP方法检测121例手术切除的乳腺癌组织中Pgp、MT的表达。

Northern blot revealed that DAT is expressed weaklt but specificlly in the brain tissue, and in situ hybridization DAT is restrictedly expressed in the adult brain tissues including substantia nigra and striatum, suggesting an important function in the dopaminergic system.

利用cRNA探针的原位杂交组织化学研究，发现DRP在成年大鼠脑组织神经元和胶质细胞均有表达，以胶质细胞广泛表达是其重要特征；而DAT在成年脑组织仅限于少部分核团的有限表达，包括黑质和纹状体。

The central part was composed of hypertrophic chondrocytes, for which the number increased with time. The cartilaginous tissue at the time of 2 weeks' cultivation appeared strongly positive staining of collagen type Ⅱ immunohistochemistry and safranine "O", as well as strongly metachromatic to toluidine blue.

其中心为肥大软骨细胞，且随培养时间的延长而增多。2周时，软骨组织甲苯胺蓝染色呈强的红色异染反应，Ⅱ型胶原免疫组织化学染色呈强阳性，番红"O"染色呈强阳性。

MAIN OUTCOME MEASURES: At 15 days after model preparation, one rat was obtained from each group to underdone morphological observation. At 21 and 30 days after model preparation, 8 rats were killed in each group, hematoxylin-eosin staining and immunohistochemical method were used to detect the expression of the proliferating cell nuclear antigen, and to calculate fibroblast proliferation index.

主要观察指标：于造模后15 d各组取1只大鼠行组织形态学观察；造模后21，30 d，每组各处死8只大鼠，取材后，行苏木精-伊红染色，免疫组织化学检测增殖细胞核抗原的表达，并计算成纤维细胞的增殖指数。

Intimal areas were measured using morphometric analysis of perfusion-fixed vein graft specimens, and intimal thickness was calculated using circumferential measurements. The SMC proliferation was studied by the immunohistochemical detection of proliferating cell nuclear antigen. Expression of MMP-2、MMP-9 mRNA in vein grafts and unoperated control Vein grafts was detected by reverse transcription polymerase chain reaction. Substrate gel zymography was used to determine the proteolytic activity.

分别行HE染色、Verhoeff弹性纤维染色观察组织病理变化，计算机病理图象分析系统测量新生内膜厚度及面积，免疫组织化学方法检测静脉壁增殖细胞核抗原表达以观察细胞增殖情况，半定量逆转录-聚合酶链反应检测静脉壁基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶-9(MMP-9)mRNA的表达，明胶酶谱法检测MMP-2、MMP-9活性，比较各组之间的差别。

The antibodies to cathepsin D and glial fibrillary acid protein and immunohistochemical PAP technique were used to observe the changes in microglia and astrocytes in the cerebella from NPC and normal mice of different ages.

应用组织蛋白酶D和胶质原纤维酸性蛋白抗体以及免疫组织化学方法，观察生后不同年龄的NPC和正常小鼠小脑内小胶质细胞和星形胶质细胞的变化。

Methods Rat spinal cord injury models were established by aneurysm clip compression, and nestin expression and proliferation of ependymal cells at different times were shown with pathological and immuno-histochemical staining.

应用动脉瘤夹压迫建立大鼠脊髓损伤模型，通过组织病理学及免疫组织化学方法检测不同时段室管膜细胞的反应性增生和神经外胚层多潜能细胞特异性抗原nestin的表达。

MethodsThe tissue microarray and immunohistochemistry SP methods were used to detect the MMP-13 expression in 97 cases of breast cancer and 39 cases of breast fibroadenoma and its correlation with clinicopathologic features was analyzed.

应用组织芯片技术和免疫组织化学SP法检测MMP-13在97例乳腺癌和39例乳腺纤维腺瘤中的表达情况，并分析其与临床病理参数的关系。

Objective To evaluate the liver histopathological changes of the chronic hepatitis B patients treated with lamivudine.

目的-从病理组织-学和-免疫组织化学改变的角度分析拉米夫定的疗效。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。