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It was indicated that the highlevel expressions of PCNA and VEGF might corporately accelerate the progression of gastric cancer.

本研究旨在利用免疫组织化学染色方法检测正常胃和胃癌组织中PCNA、VEGF的表达,探讨二者胃癌发生、发展中的作用。

Serial cryostat sections of five hearts and seven atria of rats were prepared.

利用荧光组织化学和组织学染色连续在同一切片上显示的方法,观察了5只大鼠整个心脏和7只大鼠心房的连续切片。

Methods: Expression levels of MMP10 gene and protein in human spontaneous abortion decidua were determined by RT-PCR, Western blotting and distribution by immunohistochemistry.

利用RT-PCR和Western blotting技术分别检测MMP10基因和蛋白在人正常蜕膜和自然流产蜕膜的表达水平、利用免疫组织化学技术检测MMP10在人正常蜕膜和自然流产蜕膜组织中的分布。

Objective To investigate the expression and significance of regulators and effector of Survivin and Caspase-3 in cervical squamous cell carcinomas.

目的:探讨宫颈鳞状细胞癌中凋亡凋控因子Survivin和Caspase-3蛋白的表达及其意义。方法:采用免疫组织化学技术检测40例宫颈鳞状细胞癌及10例正常宫颈组织中Survivin和Caspase-3的表达水平。

The method uses groovy histology and immunity to organize chemical technology, observation mars agent 6- hydroxide via celiac inject sympathetic much cling to small rat small intestine is epithelial after amine the amount of endolymph cell, cup shape cell, IgA+ oar cell, corpulent cell distributings change.

概要: 目的探究交感神经对肠道黏膜屏障的调节功能。方法采用常规组织学和免疫组织化学技术,观察经腹腔注射交感神经损毁剂6-羟多巴胺后小鼠小肠上皮内淋巴细胞、杯状细胞、IgA+浆细胞、肥大细胞的数量分布变化。

Methods: Atrial tissue was obtained from the right appendage during open surgery in 25 patients with RHD. The mRNA of ACE and ERK2 and AT1R was semi-qualified by reverse transcription-ploymerase chain reaction and normalized to the glyceraldehyde 3-phosphate dehydrogenase. Western blotting analysis was employed to examine the expression of ACE and ERK and AT1R. Atrial collagen volume fraction was detected by Masson's stain.

25例风心病二尖瓣狭窄接受外科手术者,手术时取右心耳处心房组织500mg,通过逆转录—聚合酶链反应技术,以GAPDH为内参照测量ACE、AT1R、ERK2的 mRNA变化,用Western Blotting了解ACE、AT1R、ERK在蛋白水平表达的差异,经免疫组织化学了解胶原纤维容积分数改变情况。

Objective: To study histological and histochemical characters of different parts of the ileum in mice and provide data for the study of ileum's function.

目的 研究大鼠不同节段回肠的组织学和组织化学特点,为其功能的研究提供资料。

Methods: 21 buttons of keratoconus, 7 buttons of normal cornea were detected.

材料与方法:取角膜移植术钻下的圆锥角膜组织21例,无眼病的尸体角膜7例,全部标本为石蜡包埋,免疫组织化学采用SABC法。

Methods The activity of PTK, PKCα,βI ,βII ,γ,ε,ξ and the active station of NF-κB in large bowel carcinoma tissues was detected by using membraneous conjugated method, laser scanning confocal microscopy and immuriohistochemistry respectively in the animal model of nude mouse transplantation tumors of large bowel carcinoma.

以大肠癌裸鼠移植瘤为动物模型,用膜结合法、激光共聚焦显微镜和免疫组织化学分别测定了大肠癌组织蛋白酪氨酸激酶、蛋白激酶Cα、βI、βⅡ、γ、ε和ξ的活性以及核因子-κB的活化状况。

By immunohistochemistry and semiquantitative RT-PCR analysis, we were the first to demonstrate that AdM mRNA and immunoreactive AdM exists in the rat brain distributed in cerebral cortex, paraventricular tissues, hypothalamus, mesencephalon, medulla oblongata and cerebellum, and AdM mRNA was also found in the human brain.

应用免疫组织化学和半定量RT-PCR方法,首次发现在大鼠的脑内,包括大脑皮层、室旁组织、下丘脑、中脑、延髓和小脑均有AdM mRNA及AdM的免疫活性物质存在;在人脑内也含有AdM mRNA。

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