组织化学的

The content of soluble protein rapidly increased with the embryo development after pollination for 16 days, while BiP markedly increased from 22nd day after pollination. The BiP failed to respond to heat shock. Immunohistochemical localization showed that BiP localized in plumule, primary vascular tissue and aleurone layer during embryo development.

组织化学免疫定位表明，在玉米胚发育的不同时期，BiP主要定位在胚芽端、初生维管组织和糊粉层中，提示胚在构建器官的同时，也为其功能执行准备了条件；热激不影响其定位。

METHODS: Adult male Wistar rats, 9 weeks of age, were injected intraperitoneally with STZ to create diabetes. At 2 week after the models were created, BDNF protein was administered into the vitreous cavities of rats. At 4 week after the models were created, the rats were killed and the retina was removed for Western blotting and Whole-mount immunohistochemistry for TH to observe the changes of TH and dopaminergic amacrine cells in retina.

9周龄Wistar大鼠，链脲佐菌素（streptozotocin，STZ）腹腔注射，制成糖尿病模型，于模型建立2wk后，大鼠眼玻璃体内注射BDNF溶液，于模型建立4wk后，处死大鼠，取出眼球，取视网膜组织进行Western blotting及视网膜铺片免疫组织化学染色检测，观察视网膜中TH水平的变化，从而反映糖尿病大鼠视网膜中多巴胺能无长突细胞水平的变化。

Methods Tissue arrayer was used to create a 252-dot tissue microarray, consisting of 84 cases of malignant lymphomas arising in esophagus, stomach, intestines, thyroid, saliva gland , orbital cavity and lung, Seven cases of diffuse large B cell lymphomas,9 adenocarcinomas, 1 neuroendocrine tumor,1 squamous carcinoma, 6 T-cell lymphomas and 18 reactive lymphoid nodes were also included as control in the microarray.

建立包含84例MALT型淋巴瘤、7例弥漫性大B细胞淋巴瘤、9例腺癌、1例神经内分泌肿瘤、1例鳞癌、6例T细胞性非何杰金淋巴瘤和18例正常淋巴结共252点阵的组织芯片，应用免疫组织化学检测Ki-67、TopoⅡα、p53、Bcl-2、Bax、C-myc和Bcl-10在MALT型淋巴瘤中的表达情况。

METHODS: Autologous whole blood was injected into the vitreous cavity of albino rabbits after canalization, thus induced experimental PVR model. Concentration of PDGF.

采用眼穿通伤+注血法制备PVR模型，进行眼底及常规组织病理学观察，ELISA法检测玻璃体中血小板源性生长因子的浓度，用免疫组织化学方法观察增生膜与血管内皮细胞表面血管细胞粘附分子-1（VCAM-1）及细胞间粘附分子-1（ICAM-1）的表达。

The immunohistochemical ABC method was used to examine the expression of cathepsin D and nm23 protein in 43 cases of breast cancer.

采用免疫组织化学ABC法检测了43例乳腺癌中的组织蛋白酶D和nm23基因蛋白的表达。

Purpose In the present study, the protective effect of SOD on the paraquat -induced inflammatory injury in hamster cheek pouch mucosa was assessed. Furthermore. a series of experiments were performed to study the mechanisms of SOD anti-inflammatory action in this study.

（1）通过常规组织病理学观察、图像分析系统等技术，评价SOD防治金黄地鼠口腔粘膜炎性损害的效果；（2）应用电子自旋共振、免疫组织化学、放射免疫法等技术，探讨SOD可能的抗炎机理。

To observe the expression of Bcl-2 and Bax in rat normal and inflammatory dental pulp in situ, and to explore the impact of Bcl-2 and Bax on pulpitis.

利用 SD大鼠的下颌第一磨牙采用内毒素化学诱导法建立牙髓炎模型，用免疫组织化学染色的方法研究Bcl-2和Bax的表达及其意义。

Roentgenographic and histologic observation confirmed that bBMP had efficient bone morphogenetic activity.

论文第一部分参照杨连甲的方法从小牛骨基质中提取bBMP，经SDS-PAGE电泳分析，bBMP保留有BMP活性蛋白成分，植入小鼠股部肌肉后的X线及组织学观察发现，所提取的bBMP具有较高的骨诱导活性，诱导成骨的时间和过程与以往的报道一致。bBMP诱导骨形成过程呈现连续又具显著的阶段性，本研究结合组织学和组织化学观察的特点，将其全过程分为四个时期。

It was showed that pectenolone and pectenoxanthin were all found in Gonads, hepatopancreas, mantle and gill, among of them, ovary, hepatopancreas and gill possessed more carotenoid than other tissues, but no carotenoids were found in the white muscles of Yesso scallops. Hepatopancreas contains a variety other carotenoids some kind of other not identified beside pectenolone and pectenoxanthin. The result also showed that the Yesso scallop with orange muscle had more the carotenoids in a variety of tissues than the common, and the female more than the male.4 Impacts of carotenoid accumulation on morphological structure and chemical composition in orange muscle of Yesso scallopThere was no difference between the orange and white muscle obserced by conventional HE staining, while musclular fiber type and matrix density in the orange muscle was different from white ones by the TEM observation of ultra-thin slices, and there were three musclular fiber types, among of them, the loose muscular fiber was orange muscle specifically.

利用HPLC法对橘红/白色闭壳肌虾夷扇贝各组织中含有的类胡萝卜素种类和含量，结果表明，虾夷扇贝在性腺、肝胰腺、外套膜、鳃丝中均含有类胡萝卜素pectenolone和pectenoxanthin，白色闭壳肌中未检测到类胡萝卜素，其中肝胰腺除上述两种类胡萝卜素外，还含有其他多种未鉴定的类胡萝卜素；在各组织中，雌性性腺、肝胰腺、鳃丝中类胡萝卜素的含量较高；橘红色闭壳肌虾夷扇贝各组织中的类胡萝卜素含量高于白色闭壳肌虾夷扇贝；雌性虾夷扇贝中的类胡萝卜素的含量高于雄性。4累积类胡萝卜素对虾夷扇贝闭壳肌的形态结构和化学成分的影响本文对橘红色闭壳肌进行了常规HE染色切片观察，并未观察到异常现象；超薄切片透射电镜观察发现橘红色闭壳肌在肌纤维类型及基质密度等方面有别于白色闭壳肌，在橘红色闭壳肌中有三种肌纤维，其中疏松型肌纤维为橘红色闭壳肌特有。

This research used Hepato-pancreas of Ctenopharyngodon idellus as experimental material to study toxicological effect of olaquindox on liver cell and pancreas exocrine cell of Ctenopharyngodon idellus, using the method of histochemisiry, primary cell culture, and immunocytochemistry, and the cells were observed by TEM and LSCM.

本研究以草鱼肝胰脏(Hepato-pancreas)为试验材料，通过原代细胞培养，组织化学，免疫荧光细胞化学等实验方法，运用透射电子显微镜，激光共聚焦扫描显微镜等手段，观察三种不同浓度的喹乙醇对体外原代培养的草鱼肝细胞和胰腺外分泌部细胞的毒理影响。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。